Seawater carbonate chemistry and gene expression and heart rates of intertidal limpet
Understanding physiological responses of organisms to warming and ocean acidification is the first step towards predicting the potential population- and community-level ecological impacts of these stressors. Increasingly, physiological plasticity is being recognized as important for organisms to ada...
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PANGAEA
2018
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Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.924364 https://doi.org/10.1594/PANGAEA.924364 |
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.924364 |
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openpolar |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2calc Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cellana toreuma Coast and continental shelf Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression standard error Gene expression (incl. proteomics) Gene name Heart rate Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH qPCR counts |
spellingShingle |
Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2calc Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cellana toreuma Coast and continental shelf Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression standard error Gene expression (incl. proteomics) Gene name Heart rate Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH qPCR counts Wang, Jie Russell, Bayden D Ding, Mengwen Dong, Yunwei Seawater carbonate chemistry and gene expression and heart rates of intertidal limpet |
topic_facet |
Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2calc Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cellana toreuma Coast and continental shelf Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression standard error Gene expression (incl. proteomics) Gene name Heart rate Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH qPCR counts |
description |
Understanding physiological responses of organisms to warming and ocean acidification is the first step towards predicting the potential population- and community-level ecological impacts of these stressors. Increasingly, physiological plasticity is being recognized as important for organisms to adapt to the changing microclimates. Here, we evaluate the importance of physiological plasticity for coping with ocean acidification and elevated temperature, and its variability among individuals, of the intertidal limpet Cellana toreuma from the same population in Xiamen. Limpets were collected from shaded mid-intertidal rock surfaces. They were acclimated under combinations of different pCO2 concentrations (400 and 1000 ppm, corresponding to a pH of 8.1 and 7.8) and temperatures (20 and 24 °C) in a short-term period (7 days), with the control conditions (20 °C and 400 ppm) representing the average annual temperature and present-day pCO2 level at the collection site. Heart rates (as a proxy for metabolic performance) and expression of genes encoding inducible and constitutive heat-shock proteins (hsp70 and hsc70) at different heat-shock temperatures (26, 30, 34, and 38 °C) were measured. Hsp70 and Hsc70 play important roles in protecting cells from heat stresses, but have different expression patterns, with Hsp70 significantly increased in expression during stress and Hsc70 constitutively expressed and only mildly induced during stress. Analysis of heart rate showed significantly higher temperature coefficients (Q10 rates) for limpets at 20 °C than at 24 °C and post-acclimation thermal sensitivity of limpets at 400 ppm was lower than at 1000 ppm. Expression of hsp70 linearly increased with the increasing heat-shock temperatures, with the largest slope occurring in limpets acclimated under a future scenario (24 °C and 1000 ppm pCO2). These results suggested that limpets showed increased sensitivity and stress response under future conditions. Furthermore, the increased variation in physiological response under the ... |
format |
Dataset |
author |
Wang, Jie Russell, Bayden D Ding, Mengwen Dong, Yunwei |
author_facet |
Wang, Jie Russell, Bayden D Ding, Mengwen Dong, Yunwei |
author_sort |
Wang, Jie |
title |
Seawater carbonate chemistry and gene expression and heart rates of intertidal limpet |
title_short |
Seawater carbonate chemistry and gene expression and heart rates of intertidal limpet |
title_full |
Seawater carbonate chemistry and gene expression and heart rates of intertidal limpet |
title_fullStr |
Seawater carbonate chemistry and gene expression and heart rates of intertidal limpet |
title_full_unstemmed |
Seawater carbonate chemistry and gene expression and heart rates of intertidal limpet |
title_sort |
seawater carbonate chemistry and gene expression and heart rates of intertidal limpet |
publisher |
PANGAEA |
publishDate |
2018 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.924364 https://doi.org/10.1594/PANGAEA.924364 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
Wang, Jie; Russell, Bayden D; Ding, Mengwen; Dong, Yunwei (2018): Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress. Biogeosciences, 15, 2803–2817, https://doi.org/10.5194/bg-15-2803-2018 Wang, Jie; Russell, Bayden D; Ding, Mengwen; Dong, Yunwei (2019): Data from: Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress [dataset]. Dryad, https://doi.org/10.5061/dryad.7s3m38n Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.924364 https://doi.org/10.1594/PANGAEA.924364 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.92436410.5194/bg-15-2803-201810.5061/dryad.7s3m38n |
_version_ |
1810469265351901184 |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.924364 2024-09-15T18:27:58+00:00 Seawater carbonate chemistry and gene expression and heart rates of intertidal limpet Wang, Jie Russell, Bayden D Ding, Mengwen Dong, Yunwei 2018 text/tab-separated-values, 296591 data points https://doi.pangaea.de/10.1594/PANGAEA.924364 https://doi.org/10.1594/PANGAEA.924364 en eng PANGAEA Wang, Jie; Russell, Bayden D; Ding, Mengwen; Dong, Yunwei (2018): Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress. Biogeosciences, 15, 2803–2817, https://doi.org/10.5194/bg-15-2803-2018 Wang, Jie; Russell, Bayden D; Ding, Mengwen; Dong, Yunwei (2019): Data from: Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress [dataset]. Dryad, https://doi.org/10.5061/dryad.7s3m38n Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.924364 https://doi.org/10.1594/PANGAEA.924364 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2calc Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cellana toreuma Coast and continental shelf Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression standard error Gene expression (incl. proteomics) Gene name Heart rate Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH qPCR counts dataset 2018 ftpangaea https://doi.org/10.1594/PANGAEA.92436410.5194/bg-15-2803-201810.5061/dryad.7s3m38n 2024-07-24T02:31:34Z Understanding physiological responses of organisms to warming and ocean acidification is the first step towards predicting the potential population- and community-level ecological impacts of these stressors. Increasingly, physiological plasticity is being recognized as important for organisms to adapt to the changing microclimates. Here, we evaluate the importance of physiological plasticity for coping with ocean acidification and elevated temperature, and its variability among individuals, of the intertidal limpet Cellana toreuma from the same population in Xiamen. Limpets were collected from shaded mid-intertidal rock surfaces. They were acclimated under combinations of different pCO2 concentrations (400 and 1000 ppm, corresponding to a pH of 8.1 and 7.8) and temperatures (20 and 24 °C) in a short-term period (7 days), with the control conditions (20 °C and 400 ppm) representing the average annual temperature and present-day pCO2 level at the collection site. Heart rates (as a proxy for metabolic performance) and expression of genes encoding inducible and constitutive heat-shock proteins (hsp70 and hsc70) at different heat-shock temperatures (26, 30, 34, and 38 °C) were measured. Hsp70 and Hsc70 play important roles in protecting cells from heat stresses, but have different expression patterns, with Hsp70 significantly increased in expression during stress and Hsc70 constitutively expressed and only mildly induced during stress. Analysis of heart rate showed significantly higher temperature coefficients (Q10 rates) for limpets at 20 °C than at 24 °C and post-acclimation thermal sensitivity of limpets at 400 ppm was lower than at 1000 ppm. Expression of hsp70 linearly increased with the increasing heat-shock temperatures, with the largest slope occurring in limpets acclimated under a future scenario (24 °C and 1000 ppm pCO2). These results suggested that limpets showed increased sensitivity and stress response under future conditions. Furthermore, the increased variation in physiological response under the ... Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |