Seawater carbonate chemistry and gene expression in the gills of juvenile turbot (Psetta maxima)
The data sets stem from a study which aimed to investigate the impact of chronic severe hypercapnia on the cellular stress response in the gills of juvenile turbot (Psetta maxima). The experiment was conducted in the so called respirometer, a recirculating aquaculture respirometer system (RARS) whic...
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Language: | English |
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PANGAEA
2019
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Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.920153 https://doi.org/10.1594/PANGAEA.920153 |
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.920153 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total standard deviation Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chordata CNRQs Quantitative reverse transcription PCR Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Experiment duration Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Gill general hyperplasia hyperplasia on basis of primary filament hyperplasia on distal part of secondary lamellae hypertrophied chloride cells hypertrophy of secondary lamellae lamellar clubbing not affected Laboratory experiment Microscopy Nekton North Atlantic OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH |
spellingShingle |
Alkalinity total standard deviation Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chordata CNRQs Quantitative reverse transcription PCR Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Experiment duration Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Gill general hyperplasia hyperplasia on basis of primary filament hyperplasia on distal part of secondary lamellae hypertrophied chloride cells hypertrophy of secondary lamellae lamellar clubbing not affected Laboratory experiment Microscopy Nekton North Atlantic OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Hermann, Bernd Timo Würtz, Sven Vanselow, Klaus Heinrich Schulz, Carsten Stiller, Kevin Torben Seawater carbonate chemistry and gene expression in the gills of juvenile turbot (Psetta maxima) |
topic_facet |
Alkalinity total standard deviation Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chordata CNRQs Quantitative reverse transcription PCR Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Experiment duration Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Gill general hyperplasia hyperplasia on basis of primary filament hyperplasia on distal part of secondary lamellae hypertrophied chloride cells hypertrophy of secondary lamellae lamellar clubbing not affected Laboratory experiment Microscopy Nekton North Atlantic OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH |
description |
The data sets stem from a study which aimed to investigate the impact of chronic severe hypercapnia on the cellular stress response in the gills of juvenile turbot (Psetta maxima). The experiment was conducted in the so called respirometer, a recirculating aquaculture respirometer system (RARS) which allowed the persistent, stable manipulation of carbon dioxide concentrations. Juvenile turbot (Psetta maxima) where subjected to different levels of environmental hypercapnia (low: ~3000 μatm, medium: ~15000 μatm, high: ~25000 μatm). After eight weeks, fish where sacrificed and gill samples were taken for further histological and transcriptional analysis. For histological investigation of the gills, samples where fixed in 4% phosphate-buffered formalin and examined via a microscope equipped with a digital camera. In order to investigate changes in the transcriptome, a fraction of the gill samples was stored in RNAlater. Relative gene expression analysis was conducted via RT-qPCR, using a Fluidigm chip system. Calibrated normalized relative quantities (CNRQs) where assessed with the QBASE software. This included the evaluation of the most suitable combination of reference genes with the help of the GeNorm algorithm. |
format |
Dataset |
author |
Hermann, Bernd Timo Würtz, Sven Vanselow, Klaus Heinrich Schulz, Carsten Stiller, Kevin Torben |
author_facet |
Hermann, Bernd Timo Würtz, Sven Vanselow, Klaus Heinrich Schulz, Carsten Stiller, Kevin Torben |
author_sort |
Hermann, Bernd Timo |
title |
Seawater carbonate chemistry and gene expression in the gills of juvenile turbot (Psetta maxima) |
title_short |
Seawater carbonate chemistry and gene expression in the gills of juvenile turbot (Psetta maxima) |
title_full |
Seawater carbonate chemistry and gene expression in the gills of juvenile turbot (Psetta maxima) |
title_fullStr |
Seawater carbonate chemistry and gene expression in the gills of juvenile turbot (Psetta maxima) |
title_full_unstemmed |
Seawater carbonate chemistry and gene expression in the gills of juvenile turbot (Psetta maxima) |
title_sort |
seawater carbonate chemistry and gene expression in the gills of juvenile turbot (psetta maxima) |
publisher |
PANGAEA |
publishDate |
2019 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.920153 https://doi.org/10.1594/PANGAEA.920153 |
genre |
North Atlantic Ocean acidification Turbot |
genre_facet |
North Atlantic Ocean acidification Turbot |
op_relation |
Hermann, Bernd Timo; Würtz, Sven; Vanselow, Klaus Heinrich; Schulz, Carsten; Stiller, Kevin Torben (2019): Divergent gene expression in the gills of juvenile turbot (Psetta maxima) exposed to chronic severe hypercapnia indicates dose-dependent increase in intracellular oxidative stress and hypoxia. Aquatic Toxicology, 206, 72-80, https://doi.org/10.1016/j.aquatox.2018.10.023 Hermann, Bernd Timo; Würtz, Sven; Vanselow, Klaus Heinrich; Schulz, Carsten; Stiller, Kevin Torben (2018): Histological and relative gene expression data obtained from the gills of juvenile turbot (Psetta maxima) after the exposure to varying degrees of severe environmental hypercapnia for 8 weeks. PANGAEA, https://doi.org/10.1594/PANGAEA.893884 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.920153 https://doi.org/10.1594/PANGAEA.920153 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.920153 https://doi.org/10.1016/j.aquatox.2018.10.023 https://doi.org/10.1594/PANGAEA.893884 |
_version_ |
1766135958424518656 |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.920153 2023-05-15T17:36:28+02:00 Seawater carbonate chemistry and gene expression in the gills of juvenile turbot (Psetta maxima) Hermann, Bernd Timo Würtz, Sven Vanselow, Klaus Heinrich Schulz, Carsten Stiller, Kevin Torben 2019-07-15 text/tab-separated-values, 4447 data points https://doi.pangaea.de/10.1594/PANGAEA.920153 https://doi.org/10.1594/PANGAEA.920153 en eng PANGAEA Hermann, Bernd Timo; Würtz, Sven; Vanselow, Klaus Heinrich; Schulz, Carsten; Stiller, Kevin Torben (2019): Divergent gene expression in the gills of juvenile turbot (Psetta maxima) exposed to chronic severe hypercapnia indicates dose-dependent increase in intracellular oxidative stress and hypoxia. Aquatic Toxicology, 206, 72-80, https://doi.org/10.1016/j.aquatox.2018.10.023 Hermann, Bernd Timo; Würtz, Sven; Vanselow, Klaus Heinrich; Schulz, Carsten; Stiller, Kevin Torben (2018): Histological and relative gene expression data obtained from the gills of juvenile turbot (Psetta maxima) after the exposure to varying degrees of severe environmental hypercapnia for 8 weeks. PANGAEA, https://doi.org/10.1594/PANGAEA.893884 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.920153 https://doi.org/10.1594/PANGAEA.920153 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Alkalinity total standard deviation Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chordata CNRQs Quantitative reverse transcription PCR Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Experiment duration Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Gill general hyperplasia hyperplasia on basis of primary filament hyperplasia on distal part of secondary lamellae hypertrophied chloride cells hypertrophy of secondary lamellae lamellar clubbing not affected Laboratory experiment Microscopy Nekton North Atlantic OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Dataset 2019 ftpangaea https://doi.org/10.1594/PANGAEA.920153 https://doi.org/10.1016/j.aquatox.2018.10.023 https://doi.org/10.1594/PANGAEA.893884 2023-01-20T09:13:47Z The data sets stem from a study which aimed to investigate the impact of chronic severe hypercapnia on the cellular stress response in the gills of juvenile turbot (Psetta maxima). The experiment was conducted in the so called respirometer, a recirculating aquaculture respirometer system (RARS) which allowed the persistent, stable manipulation of carbon dioxide concentrations. Juvenile turbot (Psetta maxima) where subjected to different levels of environmental hypercapnia (low: ~3000 μatm, medium: ~15000 μatm, high: ~25000 μatm). After eight weeks, fish where sacrificed and gill samples were taken for further histological and transcriptional analysis. For histological investigation of the gills, samples where fixed in 4% phosphate-buffered formalin and examined via a microscope equipped with a digital camera. In order to investigate changes in the transcriptome, a fraction of the gill samples was stored in RNAlater. Relative gene expression analysis was conducted via RT-qPCR, using a Fluidigm chip system. Calibrated normalized relative quantities (CNRQs) where assessed with the QBASE software. This included the evaluation of the most suitable combination of reference genes with the help of the GeNorm algorithm. Dataset North Atlantic Ocean acidification Turbot PANGAEA - Data Publisher for Earth & Environmental Science |