Seawater carbonate chemistry and expression of c-Fos protein in the brain of marine medaka
Altered behaviors have been reported in many marine fish following exposure to high CO2 concentrations. However, the mechanistic link between elevated CO2 and activation of brain regions in fish is unknown. Herein, we examined the relative quantification and location of c-Fos expression in marine me...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.920009 2023-05-15T17:51:12+02:00 Seawater carbonate chemistry and expression of c-Fos protein in the brain of marine medaka Wang, Xiaojie Lv, Yutao Xie, Jinling Li, Baolin Zhou, Tangjian Chen, Yaqi Chen, Yi Song, Jiakun 2020-07-10 text/tab-separated-values, 1337 data points https://doi.pangaea.de/10.1594/PANGAEA.920009 https://doi.org/10.1594/PANGAEA.920009 en eng PANGAEA Wang, Xiaojie; Lv, Yutao; Xie, Jinling; Li, Baolin; Zhou, Tangjian; Chen, Yaqi; Chen, Yi; Song, Jiakun (2020): Brain regions of marine medaka activated by acute and short-term ocean acidification. Science of the Total Environment, 720, 137279, https://doi.org/10.1016/j.scitotenv.2020.137279 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.920009 https://doi.org/10.1594/PANGAEA.920009 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Alkalinity total standard deviation Animalia Aragonite saturation state Behaviour Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chordata Coast and continental shelf Experiment duration Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression Gene expression (incl. proteomics) Laboratory experiment Nekton North Pacific OA-ICC Ocean Acidification International Coordination Centre Oryzias melastigma Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Potentiometric Potentiometric titration Proportion Proportion of time Registration number of species Salinity Single species Species Speed Dataset 2020 ftpangaea https://doi.org/10.1594/PANGAEA.920009 https://doi.org/10.1016/j.scitotenv.2020.137279 2023-01-20T09:13:47Z Altered behaviors have been reported in many marine fish following exposure to high CO2 concentrations. However, the mechanistic link between elevated CO2 and activation of brain regions in fish is unknown. Herein, we examined the relative quantification and location of c-Fos expression in marine medaka following acute (360 min) and short-term (7 d) exposure to CO2-enriched water (1000 ppm and 1800 ppm CO2). In the control and two treatment groups, pH was stable at 8.21, 7.92 and 7.64, respectively. After acute exposure to seawater acidified by enrichment with CO2, there was a clear upregulation of c-Fos protein in the medaka brain (P < 0.05). c-Fos protein expression peaked after 120 min exposure in the two treatment groups and thereafter began to decline. There were marked increases in c-Fos-labeling in the ventricular and periventricular zones of the cerebral hemispheres and the medulla oblongata. After 1800 ppm CO2 exposure for 7 d, medaka showed significant preference for dark zones during the initial 2 min period. c-Fos protein expression in the ventricular and periventricular zones of the diencephalon in medaka exposed to 1000 ppm and 1800 ppm CO2 were 0.51 ± 0.10 and 1.34 ± 0.30, respectively, which were significantly higher than controls (P < 0.05). Highest doublecortin protein expression occurred in theventricular zones of the diencephalon and mesencephalon. These findings suggest that the ventricular and periventricular zones of the cerebral hemispheres and the medulla oblongata of marine medaka are involved in rapid acid-base regulation. Prolonged ocean acidification may induce cell mitosis and differentiation in the adult medaka brain. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science Pacific |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total standard deviation Animalia Aragonite saturation state Behaviour Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chordata Coast and continental shelf Experiment duration Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression Gene expression (incl. proteomics) Laboratory experiment Nekton North Pacific OA-ICC Ocean Acidification International Coordination Centre Oryzias melastigma Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Potentiometric Potentiometric titration Proportion Proportion of time Registration number of species Salinity Single species Species Speed |
spellingShingle |
Alkalinity total standard deviation Animalia Aragonite saturation state Behaviour Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chordata Coast and continental shelf Experiment duration Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression Gene expression (incl. proteomics) Laboratory experiment Nekton North Pacific OA-ICC Ocean Acidification International Coordination Centre Oryzias melastigma Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Potentiometric Potentiometric titration Proportion Proportion of time Registration number of species Salinity Single species Species Speed Wang, Xiaojie Lv, Yutao Xie, Jinling Li, Baolin Zhou, Tangjian Chen, Yaqi Chen, Yi Song, Jiakun Seawater carbonate chemistry and expression of c-Fos protein in the brain of marine medaka |
topic_facet |
Alkalinity total standard deviation Animalia Aragonite saturation state Behaviour Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chordata Coast and continental shelf Experiment duration Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression Gene expression (incl. proteomics) Laboratory experiment Nekton North Pacific OA-ICC Ocean Acidification International Coordination Centre Oryzias melastigma Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Potentiometric Potentiometric titration Proportion Proportion of time Registration number of species Salinity Single species Species Speed |
description |
Altered behaviors have been reported in many marine fish following exposure to high CO2 concentrations. However, the mechanistic link between elevated CO2 and activation of brain regions in fish is unknown. Herein, we examined the relative quantification and location of c-Fos expression in marine medaka following acute (360 min) and short-term (7 d) exposure to CO2-enriched water (1000 ppm and 1800 ppm CO2). In the control and two treatment groups, pH was stable at 8.21, 7.92 and 7.64, respectively. After acute exposure to seawater acidified by enrichment with CO2, there was a clear upregulation of c-Fos protein in the medaka brain (P < 0.05). c-Fos protein expression peaked after 120 min exposure in the two treatment groups and thereafter began to decline. There were marked increases in c-Fos-labeling in the ventricular and periventricular zones of the cerebral hemispheres and the medulla oblongata. After 1800 ppm CO2 exposure for 7 d, medaka showed significant preference for dark zones during the initial 2 min period. c-Fos protein expression in the ventricular and periventricular zones of the diencephalon in medaka exposed to 1000 ppm and 1800 ppm CO2 were 0.51 ± 0.10 and 1.34 ± 0.30, respectively, which were significantly higher than controls (P < 0.05). Highest doublecortin protein expression occurred in theventricular zones of the diencephalon and mesencephalon. These findings suggest that the ventricular and periventricular zones of the cerebral hemispheres and the medulla oblongata of marine medaka are involved in rapid acid-base regulation. Prolonged ocean acidification may induce cell mitosis and differentiation in the adult medaka brain. |
format |
Dataset |
author |
Wang, Xiaojie Lv, Yutao Xie, Jinling Li, Baolin Zhou, Tangjian Chen, Yaqi Chen, Yi Song, Jiakun |
author_facet |
Wang, Xiaojie Lv, Yutao Xie, Jinling Li, Baolin Zhou, Tangjian Chen, Yaqi Chen, Yi Song, Jiakun |
author_sort |
Wang, Xiaojie |
title |
Seawater carbonate chemistry and expression of c-Fos protein in the brain of marine medaka |
title_short |
Seawater carbonate chemistry and expression of c-Fos protein in the brain of marine medaka |
title_full |
Seawater carbonate chemistry and expression of c-Fos protein in the brain of marine medaka |
title_fullStr |
Seawater carbonate chemistry and expression of c-Fos protein in the brain of marine medaka |
title_full_unstemmed |
Seawater carbonate chemistry and expression of c-Fos protein in the brain of marine medaka |
title_sort |
seawater carbonate chemistry and expression of c-fos protein in the brain of marine medaka |
publisher |
PANGAEA |
publishDate |
2020 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.920009 https://doi.org/10.1594/PANGAEA.920009 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
Wang, Xiaojie; Lv, Yutao; Xie, Jinling; Li, Baolin; Zhou, Tangjian; Chen, Yaqi; Chen, Yi; Song, Jiakun (2020): Brain regions of marine medaka activated by acute and short-term ocean acidification. Science of the Total Environment, 720, 137279, https://doi.org/10.1016/j.scitotenv.2020.137279 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.920009 https://doi.org/10.1594/PANGAEA.920009 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.920009 https://doi.org/10.1016/j.scitotenv.2020.137279 |
_version_ |
1766158278269599744 |