The effects of hypoxia/reoxygenation stress on activities of mitochondrial complexes I and IV and activities of protein kinases and phosphatases in marine bivalves
Hypoxia commonly occurs in aquatic habitats and puts strong pressure on the benthic sessile organisms unable to escape the oxygen fluctuations. Here we focused on the effects of hypoxia/reoxygenation (H/R) on activities of the mitochondrial Complexes I and IV in hypoxia-tolerant marine bivalves, nam...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.919121 2023-05-15T15:11:30+02:00 The effects of hypoxia/reoxygenation stress on activities of mitochondrial complexes I and IV and activities of protein kinases and phosphatases in marine bivalves Sokolova, Inna Falfushynska, Halina Sokolov, Eugene Piontkivska, Helen 2020-06-18 application/zip, 4 datasets https://doi.pangaea.de/10.1594/PANGAEA.919121 https://doi.org/10.1594/PANGAEA.919121 en eng PANGAEA Sokolova, Inna M; et al. (accepted): THE ROLE OF REVERSIBLE PROTEIN PHOSPHORYLATION IN REGULATION OF THE MITOCHONDRIAL ELECTRON TRANSPORT SYSTEM DURING HYPOXIA AND REOXYGENATION STRESS IN MARINE BIVALVES. Frontiers in Marine Science https://doi.pangaea.de/10.1594/PANGAEA.919121 https://doi.org/10.1594/PANGAEA.919121 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY hypoxia metabolic regulation mitochondrial electron transport system OXPHOS posttranslational modification reversible protein phosphorylation Dataset 2020 ftpangaea https://doi.org/10.1594/PANGAEA.919121 2023-01-20T07:34:35Z Hypoxia commonly occurs in aquatic habitats and puts strong pressure on the benthic sessile organisms unable to escape the oxygen fluctuations. Here we focused on the effects of hypoxia/reoxygenation (H/R) on activities of the mitochondrial Complexes I and IV in hypoxia-tolerant marine bivalves, namely, the blue mussel Mytilus edulis, the Arctic quahog Arctica islandica and the Pacific oyster Crassostrea gigas, exposing them for one or six days to extreme hypoxia (<0.1% O2) followed by one hour of reoxygenation. Furthermore, we examined the potential role of the posttranslational modifications (PTM) by reversible protein phosphorylation in regulation of the Complex I and IV activities and in the mitochondrial responses to H/R stress. The overall level of protein phosphorylation by PKA (serine/threonine protein kinase A) and PKC (protein kinase C) was measured to test for possible association between the H/R-induced changes in the PKA and PKC activities and the respective protein products of these kinases. Our results showed a strong species-specific modulation of the PKA and PKC activities by H/R stress. In the mussels and quahogs, phosphorylation by PKA and PKC led to an increase in activity of Complexes I and IV, whereas in oysters these enzymes were insensitive to PKA and PKC activation. Non-site-specific dephosphoryaltion strongly suppressed the activity of Complex I and IV in all three studied species. Dataset Arctic Arctica islandica Crassostrea gigas Pacific oyster PANGAEA - Data Publisher for Earth & Environmental Science Arctic Pacific |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
hypoxia metabolic regulation mitochondrial electron transport system OXPHOS posttranslational modification reversible protein phosphorylation |
spellingShingle |
hypoxia metabolic regulation mitochondrial electron transport system OXPHOS posttranslational modification reversible protein phosphorylation Sokolova, Inna Falfushynska, Halina Sokolov, Eugene Piontkivska, Helen The effects of hypoxia/reoxygenation stress on activities of mitochondrial complexes I and IV and activities of protein kinases and phosphatases in marine bivalves |
topic_facet |
hypoxia metabolic regulation mitochondrial electron transport system OXPHOS posttranslational modification reversible protein phosphorylation |
description |
Hypoxia commonly occurs in aquatic habitats and puts strong pressure on the benthic sessile organisms unable to escape the oxygen fluctuations. Here we focused on the effects of hypoxia/reoxygenation (H/R) on activities of the mitochondrial Complexes I and IV in hypoxia-tolerant marine bivalves, namely, the blue mussel Mytilus edulis, the Arctic quahog Arctica islandica and the Pacific oyster Crassostrea gigas, exposing them for one or six days to extreme hypoxia (<0.1% O2) followed by one hour of reoxygenation. Furthermore, we examined the potential role of the posttranslational modifications (PTM) by reversible protein phosphorylation in regulation of the Complex I and IV activities and in the mitochondrial responses to H/R stress. The overall level of protein phosphorylation by PKA (serine/threonine protein kinase A) and PKC (protein kinase C) was measured to test for possible association between the H/R-induced changes in the PKA and PKC activities and the respective protein products of these kinases. Our results showed a strong species-specific modulation of the PKA and PKC activities by H/R stress. In the mussels and quahogs, phosphorylation by PKA and PKC led to an increase in activity of Complexes I and IV, whereas in oysters these enzymes were insensitive to PKA and PKC activation. Non-site-specific dephosphoryaltion strongly suppressed the activity of Complex I and IV in all three studied species. |
format |
Dataset |
author |
Sokolova, Inna Falfushynska, Halina Sokolov, Eugene Piontkivska, Helen |
author_facet |
Sokolova, Inna Falfushynska, Halina Sokolov, Eugene Piontkivska, Helen |
author_sort |
Sokolova, Inna |
title |
The effects of hypoxia/reoxygenation stress on activities of mitochondrial complexes I and IV and activities of protein kinases and phosphatases in marine bivalves |
title_short |
The effects of hypoxia/reoxygenation stress on activities of mitochondrial complexes I and IV and activities of protein kinases and phosphatases in marine bivalves |
title_full |
The effects of hypoxia/reoxygenation stress on activities of mitochondrial complexes I and IV and activities of protein kinases and phosphatases in marine bivalves |
title_fullStr |
The effects of hypoxia/reoxygenation stress on activities of mitochondrial complexes I and IV and activities of protein kinases and phosphatases in marine bivalves |
title_full_unstemmed |
The effects of hypoxia/reoxygenation stress on activities of mitochondrial complexes I and IV and activities of protein kinases and phosphatases in marine bivalves |
title_sort |
effects of hypoxia/reoxygenation stress on activities of mitochondrial complexes i and iv and activities of protein kinases and phosphatases in marine bivalves |
publisher |
PANGAEA |
publishDate |
2020 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.919121 https://doi.org/10.1594/PANGAEA.919121 |
geographic |
Arctic Pacific |
geographic_facet |
Arctic Pacific |
genre |
Arctic Arctica islandica Crassostrea gigas Pacific oyster |
genre_facet |
Arctic Arctica islandica Crassostrea gigas Pacific oyster |
op_relation |
Sokolova, Inna M; et al. (accepted): THE ROLE OF REVERSIBLE PROTEIN PHOSPHORYLATION IN REGULATION OF THE MITOCHONDRIAL ELECTRON TRANSPORT SYSTEM DURING HYPOXIA AND REOXYGENATION STRESS IN MARINE BIVALVES. Frontiers in Marine Science https://doi.pangaea.de/10.1594/PANGAEA.919121 https://doi.org/10.1594/PANGAEA.919121 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.919121 |
_version_ |
1766342348872089600 |