Growth response of the barnacle Balanus improvisus and the bryozoan Electra pilosa from Kiel Fjord to biogenic pH fluctuations and global ocean acidification scenarios
We investigated the effect of pH fluctuations, generated by the brown alga Fucus serratus' biological activity, on the calcifying epibionts Balanus improvisus and Electra pilosa in two temperatures (10 °C and 15 °C) and under ocean acidification (OA). For this, epibionts were grown on inactive...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.917864 2024-09-15T18:27:55+00:00 Growth response of the barnacle Balanus improvisus and the bryozoan Electra pilosa from Kiel Fjord to biogenic pH fluctuations and global ocean acidification scenarios Johnson, Mildred Jessica Hennigs, Laura Margarethe Pansch, Christian Wall, Marlene 2020 application/zip, 2 datasets https://doi.pangaea.de/10.1594/PANGAEA.917864 https://doi.org/10.1594/PANGAEA.917864 en eng PANGAEA Johnson, Mildred Jessica; Hennigs, Laura Margarethe; Sawall, Yvonne; Pansch, Christian; Wall, Marlene (2020): Growth response of calcifying marine epibionts to biogenic pH fluctuations and global ocean acidification scenarios. Limnology and Oceanography, 9999, 1-14, https://doi.org/10.1002/lno.11669 https://doi.pangaea.de/10.1594/PANGAEA.917864 https://doi.org/10.1594/PANGAEA.917864 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess fluctuations growth Ocean acidification pH dataset publication series 2020 ftpangaea https://doi.org/10.1594/PANGAEA.91786410.1002/lno.11669 2024-07-24T02:31:21Z We investigated the effect of pH fluctuations, generated by the brown alga Fucus serratus' biological activity, on the calcifying epibionts Balanus improvisus and Electra pilosa in two temperatures (10 °C and 15 °C) and under ocean acidification (OA). For this, epibionts were grown on inactive (Plexiglas) and biologically active (F. serratus) surfaces and exposed to (i) constant pH scenarios under ambient (pH 8.1) or OA conditions (pH 7.7), or (ii) oscillating pH scenarios mimicking benthic boundary layer conditions at ambient (pH 7.7-8.6) or OA conditions (pH 7.4-8.2). The initial size of barnacles (diameter of basal plate) was determined for ~50 randomly chosen individuals on Plexiglas and algal surfaces by analysing pictures taken under the microscope one day prior to the start of the experiment, using ImageJ. The resulting start sizes were 0.72 ± 0.09 mm on Plexiglass and 0.70 ± 0.09 mm on algal surfaces, respectively. Final sizes of B. improvisus were determined from pictures taken at the end of the experiment following the same procedure as for initial size measurements. Note that data of single individuals within one cylinder (independent replicate) has to be combined to a mean before analyses. For the bryozoan E. pilosa, colony growth determinations differed slightly between the two surface types. For colonies on inactive surfaces, growth (addition of new zooids) was determined by comparing the number of zooids per colony in the beginning and at the end of the experiment using photographs. For colonies on active surfaces, the number of new zooids were counted directly by counting the zooids established after calcein staining on pictures taken at the end of the experiment, under a fluorescence microscope. The different procedures were employed to minimize handling of the active surface samples. The growth efficiency (GE) of colonies on both surfaces were calculated based on the number of zooids before the experiment (Ns) and the number of new zooids (Nn) following the equation GE = log10 (Nn / Ns). Other/Unknown Material Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
fluctuations growth Ocean acidification pH |
spellingShingle |
fluctuations growth Ocean acidification pH Johnson, Mildred Jessica Hennigs, Laura Margarethe Pansch, Christian Wall, Marlene Growth response of the barnacle Balanus improvisus and the bryozoan Electra pilosa from Kiel Fjord to biogenic pH fluctuations and global ocean acidification scenarios |
topic_facet |
fluctuations growth Ocean acidification pH |
description |
We investigated the effect of pH fluctuations, generated by the brown alga Fucus serratus' biological activity, on the calcifying epibionts Balanus improvisus and Electra pilosa in two temperatures (10 °C and 15 °C) and under ocean acidification (OA). For this, epibionts were grown on inactive (Plexiglas) and biologically active (F. serratus) surfaces and exposed to (i) constant pH scenarios under ambient (pH 8.1) or OA conditions (pH 7.7), or (ii) oscillating pH scenarios mimicking benthic boundary layer conditions at ambient (pH 7.7-8.6) or OA conditions (pH 7.4-8.2). The initial size of barnacles (diameter of basal plate) was determined for ~50 randomly chosen individuals on Plexiglas and algal surfaces by analysing pictures taken under the microscope one day prior to the start of the experiment, using ImageJ. The resulting start sizes were 0.72 ± 0.09 mm on Plexiglass and 0.70 ± 0.09 mm on algal surfaces, respectively. Final sizes of B. improvisus were determined from pictures taken at the end of the experiment following the same procedure as for initial size measurements. Note that data of single individuals within one cylinder (independent replicate) has to be combined to a mean before analyses. For the bryozoan E. pilosa, colony growth determinations differed slightly between the two surface types. For colonies on inactive surfaces, growth (addition of new zooids) was determined by comparing the number of zooids per colony in the beginning and at the end of the experiment using photographs. For colonies on active surfaces, the number of new zooids were counted directly by counting the zooids established after calcein staining on pictures taken at the end of the experiment, under a fluorescence microscope. The different procedures were employed to minimize handling of the active surface samples. The growth efficiency (GE) of colonies on both surfaces were calculated based on the number of zooids before the experiment (Ns) and the number of new zooids (Nn) following the equation GE = log10 (Nn / Ns). |
format |
Other/Unknown Material |
author |
Johnson, Mildred Jessica Hennigs, Laura Margarethe Pansch, Christian Wall, Marlene |
author_facet |
Johnson, Mildred Jessica Hennigs, Laura Margarethe Pansch, Christian Wall, Marlene |
author_sort |
Johnson, Mildred Jessica |
title |
Growth response of the barnacle Balanus improvisus and the bryozoan Electra pilosa from Kiel Fjord to biogenic pH fluctuations and global ocean acidification scenarios |
title_short |
Growth response of the barnacle Balanus improvisus and the bryozoan Electra pilosa from Kiel Fjord to biogenic pH fluctuations and global ocean acidification scenarios |
title_full |
Growth response of the barnacle Balanus improvisus and the bryozoan Electra pilosa from Kiel Fjord to biogenic pH fluctuations and global ocean acidification scenarios |
title_fullStr |
Growth response of the barnacle Balanus improvisus and the bryozoan Electra pilosa from Kiel Fjord to biogenic pH fluctuations and global ocean acidification scenarios |
title_full_unstemmed |
Growth response of the barnacle Balanus improvisus and the bryozoan Electra pilosa from Kiel Fjord to biogenic pH fluctuations and global ocean acidification scenarios |
title_sort |
growth response of the barnacle balanus improvisus and the bryozoan electra pilosa from kiel fjord to biogenic ph fluctuations and global ocean acidification scenarios |
publisher |
PANGAEA |
publishDate |
2020 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.917864 https://doi.org/10.1594/PANGAEA.917864 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
Johnson, Mildred Jessica; Hennigs, Laura Margarethe; Sawall, Yvonne; Pansch, Christian; Wall, Marlene (2020): Growth response of calcifying marine epibionts to biogenic pH fluctuations and global ocean acidification scenarios. Limnology and Oceanography, 9999, 1-14, https://doi.org/10.1002/lno.11669 https://doi.pangaea.de/10.1594/PANGAEA.917864 https://doi.org/10.1594/PANGAEA.917864 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.91786410.1002/lno.11669 |
_version_ |
1810469197993476096 |