Organic matter and bacteria measurements of POLARSTERN cruise PS114 and Maria S. Merian cruise MSM77

• Main Authors: von Jackowski, Anabel, Grosse, Julia, Nöthig, Eva-Maria, Engel, Anja
• Format: Dataset
• Language: English
• Published: PANGAEA 2020
• Subjects: 3H-leucine microcentrifuge; Acetone extraction (Turner Designs); Acidic molybdate solution; Alanine; Amino acid; total hydrolysable dissolved; Arabinose; Arctic; Arginine; Aspartic acid; Bacteria; heterotrophic with relatively high DNA content; heterotrophic with relatively low DNA content; Bacterial production; Carbohydrates; dissolved combined; Carbon; organic; dissolved; Chlorophyll a; Coomassie stainable particles; CSP; CTD/Rosette; CTD-RO; Date/Time of event; DEPTH; water; Event label; FACSCalibur flow-cytometer (Becton Dickinson); FRAM; FRontiers in Arctic marine Monitoring; Fucose; Galactosamine; Galactose; Galacturonic acid; Glucosamine; Glucose; Glucuronic acid; Glutamic acid; Glycine; Greenland Sea; Hausgarten; High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD); Dionex 3000; High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization; Agilent 1260; High temperature catalytic oxidation (TOC-VCSH); Isoleucine; Greenland
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.915751; https://doi.org/10.1594/PANGAEA.915751

The discrete biogeochemical measurements from RV Polarstern PS114 and RV Maria S. Merian MSM77 sampled for dissolved organic matter, heterotrophic bacteria and gel particles. The parameters include chlorophyll, DOC/TDN/TDP/DOP, dissolved hydrolysable amino acids, dissolved combined carbohydrates, heterotrophic bacterial abundance and production, gel particles like TEP and CSP. The samples were aquired within 0-100 m of the Greenland Sea between 78°N to 80°N and 2°W to 14°E. The data was collected daily on board PS114 from 16th July 2018-23rd July 2018 and on board MSM77 from 16th September 2018-4th October 2018. The water collection occured using a CTD and laboratory methods varied by parameter. Chlorophyll was extracted using acetone, DOC/TDN was analysed using High-Temperature Catalytic Oxidation (TOC-VCSH), TDP/DOP was analysed colorimetrically using Acidic Molybdate Solution, dissolved hydrolysable amino acids were analysed using High Performal Liquid Chromatography (HPLC) Ortho-phthaldialdehyde Derivatization, dissolved combined carbohydrates were analysed using High Performance Anion Exchange Chromatography (HPAEC) coupled with Pulsed Amperometric Detection (PAD), cell abundance was analysed using flow cytometery, bacterial production was analysed using radioactively labelled 3H-Leucine and apllication of the microcentrifuge method, gel particles were analysed microscopically followed by image analysis. The purpose of data collection was to understand seasonal cycling of organic mater and heterotrophic bacteria dynamics within microbial loop.