Metadata and NCBI-Accession numbers of 16S data for seawater and Craniella sponges from the North Atlantic Ocean and an aquarium experiment in 2017 and 2018
Deep-sea sponge grounds are underexplored ecosystems that provide numerous goods and services to the functioning of the deep-sea. This study assessed the microbial diversity (by 16S rRNA gene amplicon sequencing) in embryos, juveniles and adults of Craniella zetlandica and Craniella infrequens, comm...
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Format: | Dataset |
Language: | English |
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PANGAEA
2020
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Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.913890 https://doi.org/10.1594/PANGAEA.913890 |
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.913890 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Accession number genetics Agassiz Trawl AGT amplicon sequencing AQUARIUM_C.zetlandica Area/locality Campaign Chloroflexi Craniella CTD/Rosette CTD-RO DATE/TIME Deep-sea Sponge Grounds Ecosystems of the North Atlantic DEPTH water Device type early life stages Event label EXP Experiment fluorescence in situ hybridisation G. O. Sars (2003) GS2017110 GS2017110-15-CTD-05 GS2017110-19-ROV10 GS2018108 GS2018108-17-AGT-01 GS2018108-22-CTD-07 GS2018108-64-ROV-48 GS2018108-66-CTD-16 GS2018108-70-ROV-50 GS2018108-77-CTD-24 GS2018108-78-ROV-52 Identification LATITUDE LONGITUDE Remote operated vehicle ROV Sample code/label Sample position Sample type Schultz Bank SponGES Stjernsund symbiosis vulnerable marine ecosystems Well-known text |
spellingShingle |
Accession number genetics Agassiz Trawl AGT amplicon sequencing AQUARIUM_C.zetlandica Area/locality Campaign Chloroflexi Craniella CTD/Rosette CTD-RO DATE/TIME Deep-sea Sponge Grounds Ecosystems of the North Atlantic DEPTH water Device type early life stages Event label EXP Experiment fluorescence in situ hybridisation G. O. Sars (2003) GS2017110 GS2017110-15-CTD-05 GS2017110-19-ROV10 GS2018108 GS2018108-17-AGT-01 GS2018108-22-CTD-07 GS2018108-64-ROV-48 GS2018108-66-CTD-16 GS2018108-70-ROV-50 GS2018108-77-CTD-24 GS2018108-78-ROV-52 Identification LATITUDE LONGITUDE Remote operated vehicle ROV Sample code/label Sample position Sample type Schultz Bank SponGES Stjernsund symbiosis vulnerable marine ecosystems Well-known text Busch, Kathrin Wurz, Erik Rapp, Hans Tore Bayer, Kristina Franke, Andre Hentschel, Ute Metadata and NCBI-Accession numbers of 16S data for seawater and Craniella sponges from the North Atlantic Ocean and an aquarium experiment in 2017 and 2018 |
topic_facet |
Accession number genetics Agassiz Trawl AGT amplicon sequencing AQUARIUM_C.zetlandica Area/locality Campaign Chloroflexi Craniella CTD/Rosette CTD-RO DATE/TIME Deep-sea Sponge Grounds Ecosystems of the North Atlantic DEPTH water Device type early life stages Event label EXP Experiment fluorescence in situ hybridisation G. O. Sars (2003) GS2017110 GS2017110-15-CTD-05 GS2017110-19-ROV10 GS2018108 GS2018108-17-AGT-01 GS2018108-22-CTD-07 GS2018108-64-ROV-48 GS2018108-66-CTD-16 GS2018108-70-ROV-50 GS2018108-77-CTD-24 GS2018108-78-ROV-52 Identification LATITUDE LONGITUDE Remote operated vehicle ROV Sample code/label Sample position Sample type Schultz Bank SponGES Stjernsund symbiosis vulnerable marine ecosystems Well-known text |
description |
Deep-sea sponge grounds are underexplored ecosystems that provide numerous goods and services to the functioning of the deep-sea. This study assessed the microbial diversity (by 16S rRNA gene amplicon sequencing) in embryos, juveniles and adults of Craniella zetlandica and Craniella infrequens, common and abundant representatives of deep-sea sponge grounds in the North Atlantic. For this study, in total 39 sponge individuals of the two sponge species were collected and analysed for their associated microbial community composition: C. zetlandica (8 adults, of which one was brooding, and 9 juveniles) and C. infrequens (8 adults, of which four were brooding, and 9 juveniles). We use the term 'juvenile' for small (i.e. mean diameter = 1 cm for C. infrequens; and maximal diameter = 0.3 cm for C. zetlandica), young individuals. For C. zetlandica, juveniles were sampled in September 2018 from an aquarium system (Bergen, Norway). C. zetlandica juveniles were smaller (and most likely younger) than the in situ sampled C. infrequens juveniles and therefore flash-frozen as a whole. Embryos of both sponge species were carefully picked out of the parent sponges with sterile spring steel forceps onboard the research vessel using a stereomicroscope. Ten whole embryos were pooled per adult sponge to account for the small biomass. Data such as presented here provide information on the recruitment of deep-sea sponge holobionts which is needed to develop integrated management tools of such vulnerable marine ecosystems. |
format |
Dataset |
author |
Busch, Kathrin Wurz, Erik Rapp, Hans Tore Bayer, Kristina Franke, Andre Hentschel, Ute |
author_facet |
Busch, Kathrin Wurz, Erik Rapp, Hans Tore Bayer, Kristina Franke, Andre Hentschel, Ute |
author_sort |
Busch, Kathrin |
title |
Metadata and NCBI-Accession numbers of 16S data for seawater and Craniella sponges from the North Atlantic Ocean and an aquarium experiment in 2017 and 2018 |
title_short |
Metadata and NCBI-Accession numbers of 16S data for seawater and Craniella sponges from the North Atlantic Ocean and an aquarium experiment in 2017 and 2018 |
title_full |
Metadata and NCBI-Accession numbers of 16S data for seawater and Craniella sponges from the North Atlantic Ocean and an aquarium experiment in 2017 and 2018 |
title_fullStr |
Metadata and NCBI-Accession numbers of 16S data for seawater and Craniella sponges from the North Atlantic Ocean and an aquarium experiment in 2017 and 2018 |
title_full_unstemmed |
Metadata and NCBI-Accession numbers of 16S data for seawater and Craniella sponges from the North Atlantic Ocean and an aquarium experiment in 2017 and 2018 |
title_sort |
metadata and ncbi-accession numbers of 16s data for seawater and craniella sponges from the north atlantic ocean and an aquarium experiment in 2017 and 2018 |
publisher |
PANGAEA |
publishDate |
2020 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.913890 https://doi.org/10.1594/PANGAEA.913890 |
op_coverage |
MEDIAN LATITUDE: 72.719640 * MEDIAN LONGITUDE: 12.170438 * SOUTH-BOUND LATITUDE: 70.262200 * WEST-BOUND LONGITUDE: 7.398600 * NORTH-BOUND LATITUDE: 73.836900 * EAST-BOUND LONGITUDE: 22.481400 * DATE/TIME START: 2017-07-27T00:47:52 * DATE/TIME END: 2018-09-19T00:00:00 * MINIMUM DEPTH, water: 217 m * MAXIMUM DEPTH, water: 1070 m |
long_lat |
ENVELOPE(7.398600,22.481400,73.836900,70.262200) |
geographic |
Bergen Norway |
geographic_facet |
Bergen Norway |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
Busch, Kathrin; Wurz, Erik; Rapp, Hans Tore; Bayer, Kristina; Franke, Andre; Hentschel, Ute (2020): Chloroflexi Dominate the Deep-Sea Golf Ball Sponges Craniella zetlandica and Craniella infrequens Throughout Different Life Stages. Frontiers in Marine Science, 7, https://doi.org/10.3389/fmars.2020.00674 Busch, Kathrin; Wurz, Erik; Rapp, Hans Tore; Bayer, Kristina; Franke, Andre; Hentschel, Ute (2020): In situ footage of a spawning deep-sea golf ball sponge Craniella zetlandica. PANGAEA, https://doi.org/10.1594/PANGAEA.918218 https://doi.pangaea.de/10.1594/PANGAEA.913890 https://doi.org/10.1594/PANGAEA.913890 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.913890 https://doi.org/10.3389/fmars.2020.00674 https://doi.org/10.1594/PANGAEA.918218 |
_version_ |
1766128766116954112 |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.913890 2023-05-15T17:31:16+02:00 Metadata and NCBI-Accession numbers of 16S data for seawater and Craniella sponges from the North Atlantic Ocean and an aquarium experiment in 2017 and 2018 Busch, Kathrin Wurz, Erik Rapp, Hans Tore Bayer, Kristina Franke, Andre Hentschel, Ute MEDIAN LATITUDE: 72.719640 * MEDIAN LONGITUDE: 12.170438 * SOUTH-BOUND LATITUDE: 70.262200 * WEST-BOUND LONGITUDE: 7.398600 * NORTH-BOUND LATITUDE: 73.836900 * EAST-BOUND LONGITUDE: 22.481400 * DATE/TIME START: 2017-07-27T00:47:52 * DATE/TIME END: 2018-09-19T00:00:00 * MINIMUM DEPTH, water: 217 m * MAXIMUM DEPTH, water: 1070 m 2020-03-24 text/tab-separated-values, 441 data points https://doi.pangaea.de/10.1594/PANGAEA.913890 https://doi.org/10.1594/PANGAEA.913890 en eng PANGAEA Busch, Kathrin; Wurz, Erik; Rapp, Hans Tore; Bayer, Kristina; Franke, Andre; Hentschel, Ute (2020): Chloroflexi Dominate the Deep-Sea Golf Ball Sponges Craniella zetlandica and Craniella infrequens Throughout Different Life Stages. Frontiers in Marine Science, 7, https://doi.org/10.3389/fmars.2020.00674 Busch, Kathrin; Wurz, Erik; Rapp, Hans Tore; Bayer, Kristina; Franke, Andre; Hentschel, Ute (2020): In situ footage of a spawning deep-sea golf ball sponge Craniella zetlandica. PANGAEA, https://doi.org/10.1594/PANGAEA.918218 https://doi.pangaea.de/10.1594/PANGAEA.913890 https://doi.org/10.1594/PANGAEA.913890 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Accession number genetics Agassiz Trawl AGT amplicon sequencing AQUARIUM_C.zetlandica Area/locality Campaign Chloroflexi Craniella CTD/Rosette CTD-RO DATE/TIME Deep-sea Sponge Grounds Ecosystems of the North Atlantic DEPTH water Device type early life stages Event label EXP Experiment fluorescence in situ hybridisation G. O. Sars (2003) GS2017110 GS2017110-15-CTD-05 GS2017110-19-ROV10 GS2018108 GS2018108-17-AGT-01 GS2018108-22-CTD-07 GS2018108-64-ROV-48 GS2018108-66-CTD-16 GS2018108-70-ROV-50 GS2018108-77-CTD-24 GS2018108-78-ROV-52 Identification LATITUDE LONGITUDE Remote operated vehicle ROV Sample code/label Sample position Sample type Schultz Bank SponGES Stjernsund symbiosis vulnerable marine ecosystems Well-known text Dataset 2020 ftpangaea https://doi.org/10.1594/PANGAEA.913890 https://doi.org/10.3389/fmars.2020.00674 https://doi.org/10.1594/PANGAEA.918218 2023-01-20T09:13:20Z Deep-sea sponge grounds are underexplored ecosystems that provide numerous goods and services to the functioning of the deep-sea. This study assessed the microbial diversity (by 16S rRNA gene amplicon sequencing) in embryos, juveniles and adults of Craniella zetlandica and Craniella infrequens, common and abundant representatives of deep-sea sponge grounds in the North Atlantic. For this study, in total 39 sponge individuals of the two sponge species were collected and analysed for their associated microbial community composition: C. zetlandica (8 adults, of which one was brooding, and 9 juveniles) and C. infrequens (8 adults, of which four were brooding, and 9 juveniles). We use the term 'juvenile' for small (i.e. mean diameter = 1 cm for C. infrequens; and maximal diameter = 0.3 cm for C. zetlandica), young individuals. For C. zetlandica, juveniles were sampled in September 2018 from an aquarium system (Bergen, Norway). C. zetlandica juveniles were smaller (and most likely younger) than the in situ sampled C. infrequens juveniles and therefore flash-frozen as a whole. Embryos of both sponge species were carefully picked out of the parent sponges with sterile spring steel forceps onboard the research vessel using a stereomicroscope. Ten whole embryos were pooled per adult sponge to account for the small biomass. Data such as presented here provide information on the recruitment of deep-sea sponge holobionts which is needed to develop integrated management tools of such vulnerable marine ecosystems. Dataset North Atlantic PANGAEA - Data Publisher for Earth & Environmental Science Bergen Norway ENVELOPE(7.398600,22.481400,73.836900,70.262200) |