Prokaryotic cell abundance and biomass measured during Maria S. Merian cruise MSM29 and Polarstern cruise PS85 to the Fram Strait

• Main Author: Bienhold, Christina
• Format: Dataset
• Language: English
• Published: PANGAEA 2019
• Subjects: ABYSS; Acridine Orange Direct Counting (AODC); AODC; ARK-XXVIII/2; Assessment of bacterial life and matter cycling in deep-sea surface sediments; Bacteria; biomass as carbon; cell biovolume; bacterial abundance; bacterial biomass; Carbon biomass estimation (unspec.); cell counts; Comment; Date/Time of event; deep sea sediment; DEPTH; sediment/rock; EGI; Elevation of event; Event label; Fram Strait; HAUSGARTEN 2013; HG_I; HG_II; HGI; HGIV; HGIX; HGVI; KH; Latitude of event; Longitude of event; Maria S. Merian; MSM29; MSM29_424-3; MSM29_425-3; MSM29_427-2; MSM29_432-2; MSM29_439-3; MSM29_443-2; MSM29_450-2; MUC; MultiCorer; Multicorer with television; N_4; N4; North Greenland Sea; Polarstern; Prokaryotes; abundance as single cells; Arctic; Greenland; Greenland Sea; North Greenland
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.908130; https://doi.org/10.1594/PANGAEA.908130

Prokaryotic cell abundances and biomass were determined in deep-sea sediment samples collected at the deep-sea LTER HAUSGARTEN, located in the Fram Strait. Sampling was performed during two cruises, i.e. RV Maria S. Merian MSM 29 in summer 2013 (23 June-12 July) and RV Polarstern PS85 in summer 2014 (6 June-3 July). Undisturbed sediment cores were collected with a TV-guided multicorer. Prokaryotic cell abundances were determined using acridine orange direct counts, following the procedure described in (Hoffmann et al., 2017; doi:10.3389/fmicb.2017.00266). For each sample two replicate filters were counted. Cell volumes were determined using a stage micrometer and converted to biomass using a conversion factor of 3 x 10^-13 g C /µm³ (Børsheim, K. Y., Bratbak, G., and Heldal, M. (1990). Enumeration and biomass estimation of planktonic bacteria and viruses by transmission electron microscopy. Applied and Environmental Microbiology 56, 352-356).