Microbial cell numbers measured during POLARSTERN cruise PS94 (TransArcII) to the Barents Sea and central Arctic

For the determination of prokaryotic cell numbers, 1 cm³ sediment was fixed with sterile filtered formalin/seawater at a final concentration of 2% and stored at 4°C until further analyses in the home laboratory. Samples were filtered on 0.2 µm polycarbonate filters, stained with acridine orange (Mey...

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Bibliographic Details
Main Author: Bienhold, Christina
Format: Dataset
Language:English
Published: PANGAEA 2019
Subjects:
ABYSS
Acridine Orange Direct Counting (AODC)
Arctic Ocean
ARK-XXIX/3
Assessment of bacterial life and matter cycling in deep-sea surface sediments
Barents Sea
Central Arctic Ocean
Climate change
Comment
Date/Time of event
Depth
bottom/max
sediment/rock
top/min
Elevation of event
Event label
GBG
Giant box corer
Giant box grab
GKG
Latitude of event
Longitude of event
microbial abundance
MUC
MultiCorer
organic-matter
Polarstern
Prokaryotes
abundance as single cells
PS94
PS94/001-2
PS94/002-2
PS94/021-2
PS94/032-10
PS94/066-2
PS94/087-2
PS94/101-10
PS94/105-2
PS94/123-2
PS94/130-4
PS94/149-5/6
PS94/161-6
Sediment oxygen uptake
System Laptev-Sea: Transdrift
TRANSDRIFT
Arctic
laptev
Laptev Sea
Online Access:https://doi.pangaea.de/10.1594/PANGAEA.907884
https://doi.org/10.1594/PANGAEA.907884
Description
Summary:For the determination of prokaryotic cell numbers, 1 cm³ sediment was fixed with sterile filtered formalin/seawater at a final concentration of 2% and stored at 4°C until further analyses in the home laboratory. Samples were filtered on 0.2 µm polycarbonate filters, stained with acridine orange (Meyer-Reil, 1983, doi:10.1007/bf00395813), and counted using an epifluorescence microscope (Axiophot II Imaging, Zeiss, Jena, Germany). For each sample, 30 random grids from two replicate filters were counted and averaged.

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