Seawater carbonate chemistry and physiology and toxicity of the dinoflagellate Karenia mikimotoi
This work demonstrated a 10-day batch culture experiment to test the physiology and toxicity of harmful dinoflagellate Karenia mikimotoi in response to ocean acidification (OA) under two different phosphate concentrations. Cells were previously acclimated in OA (pH = 7.8 and CO2 = 1100 μatm) conditi...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.907178 2024-09-15T18:27:55+00:00 Seawater carbonate chemistry and physiology and toxicity of the dinoflagellate Karenia mikimotoi Wang, Hong Niu, Xiaoqin Feng, Xinqian Gonçalves, Rodrigo J Guan, WanChun 2019 text/tab-separated-values, 8248 data points https://doi.pangaea.de/10.1594/PANGAEA.907178 https://doi.org/10.1594/PANGAEA.907178 en eng PANGAEA Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.907178 https://doi.org/10.1594/PANGAEA.907178 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Wang, Hong; Niu, Xiaoqin; Feng, Xinqian; Gonçalves, Rodrigo J; Guan, WanChun (2019): Effects of ocean acidification and phosphate limitation on physiology and toxicity of the dinoflagellate Karenia mikimotoi. Harmful Algae, 87, 101621, https://doi.org/10.1016/j.hal.2019.101621 Alkalinity total Aragonite saturation state Bicarbonate ion standard deviation Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cell density Chromista Deformation rate Electron transport rate relative Experiment duration Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate Haemolytic activity Identification Immunology/Self-protection Irradiance Karenia mikimotoi Laboratory experiment Macro-nutrients Myzozoa Not applicable OA-ICC dataset 2019 ftpangaea https://doi.org/10.1594/PANGAEA.90717810.1016/j.hal.2019.101621 2024-07-24T02:31:34Z This work demonstrated a 10-day batch culture experiment to test the physiology and toxicity of harmful dinoflagellate Karenia mikimotoi in response to ocean acidification (OA) under two different phosphate concentrations. Cells were previously acclimated in OA (pH = 7.8 and CO2 = 1100 μatm) condition for about three months before testing the responses of K. mikimotoi cells to a two-factorial combinations experimentation. This work measured the variation in physiological parameters (growth, rETR) and toxicity (hemolytic activity and its toxicity to zebrafish embryos) in four treatments, representing two factorial combinations of CO2 (450 and 1100 μatm) and phosphate concentration (37.75 and 4.67 umol l−1). Results: OA stimulated the faster growth, and the highest rETRmax in high phosphate (HP) treatment, low phosphate (LP) and a combination of high CO2 and low phosphate (HC*LP) inhibited the growth and Ek in comparison to low CO2*high phosphate (LCHP) treatment. The embryotoxicity of K. mikimotoi cells enhanced in all high CO2 (HC) conditions irrespective of phosphate concentration, but the EC50 of hemolytic activity increased in all high CO2 (HC) and low phosphate (LP) treatments in comparison of LCHP. Ocean acidification (high CO2 and lower pH) was probably the main factor that affected the rETRmax, hemolytic activity and embryotoxicity, but low phosphate was the main factor that affected the growth, α, and Ek. There were significant interactive effects of OA and low phosphate (LP) on growth, rETRmax, and hemolytic activity, but there were no significant effects on α, Ek, and embryotoxicity. If these results are extrapolated to the aquatic environment, it can be hypothesized that the K. mikimotoi cells were impacted significantly by future changing ocean (e.g., ocean acidification and nutrient stoichiometry). Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total Aragonite saturation state Bicarbonate ion standard deviation Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cell density Chromista Deformation rate Electron transport rate relative Experiment duration Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate Haemolytic activity Identification Immunology/Self-protection Irradiance Karenia mikimotoi Laboratory experiment Macro-nutrients Myzozoa Not applicable OA-ICC |
spellingShingle |
Alkalinity total Aragonite saturation state Bicarbonate ion standard deviation Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cell density Chromista Deformation rate Electron transport rate relative Experiment duration Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate Haemolytic activity Identification Immunology/Self-protection Irradiance Karenia mikimotoi Laboratory experiment Macro-nutrients Myzozoa Not applicable OA-ICC Wang, Hong Niu, Xiaoqin Feng, Xinqian Gonçalves, Rodrigo J Guan, WanChun Seawater carbonate chemistry and physiology and toxicity of the dinoflagellate Karenia mikimotoi |
topic_facet |
Alkalinity total Aragonite saturation state Bicarbonate ion standard deviation Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cell density Chromista Deformation rate Electron transport rate relative Experiment duration Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate Haemolytic activity Identification Immunology/Self-protection Irradiance Karenia mikimotoi Laboratory experiment Macro-nutrients Myzozoa Not applicable OA-ICC |
description |
This work demonstrated a 10-day batch culture experiment to test the physiology and toxicity of harmful dinoflagellate Karenia mikimotoi in response to ocean acidification (OA) under two different phosphate concentrations. Cells were previously acclimated in OA (pH = 7.8 and CO2 = 1100 μatm) condition for about three months before testing the responses of K. mikimotoi cells to a two-factorial combinations experimentation. This work measured the variation in physiological parameters (growth, rETR) and toxicity (hemolytic activity and its toxicity to zebrafish embryos) in four treatments, representing two factorial combinations of CO2 (450 and 1100 μatm) and phosphate concentration (37.75 and 4.67 umol l−1). Results: OA stimulated the faster growth, and the highest rETRmax in high phosphate (HP) treatment, low phosphate (LP) and a combination of high CO2 and low phosphate (HC*LP) inhibited the growth and Ek in comparison to low CO2*high phosphate (LCHP) treatment. The embryotoxicity of K. mikimotoi cells enhanced in all high CO2 (HC) conditions irrespective of phosphate concentration, but the EC50 of hemolytic activity increased in all high CO2 (HC) and low phosphate (LP) treatments in comparison of LCHP. Ocean acidification (high CO2 and lower pH) was probably the main factor that affected the rETRmax, hemolytic activity and embryotoxicity, but low phosphate was the main factor that affected the growth, α, and Ek. There were significant interactive effects of OA and low phosphate (LP) on growth, rETRmax, and hemolytic activity, but there were no significant effects on α, Ek, and embryotoxicity. If these results are extrapolated to the aquatic environment, it can be hypothesized that the K. mikimotoi cells were impacted significantly by future changing ocean (e.g., ocean acidification and nutrient stoichiometry). |
format |
Dataset |
author |
Wang, Hong Niu, Xiaoqin Feng, Xinqian Gonçalves, Rodrigo J Guan, WanChun |
author_facet |
Wang, Hong Niu, Xiaoqin Feng, Xinqian Gonçalves, Rodrigo J Guan, WanChun |
author_sort |
Wang, Hong |
title |
Seawater carbonate chemistry and physiology and toxicity of the dinoflagellate Karenia mikimotoi |
title_short |
Seawater carbonate chemistry and physiology and toxicity of the dinoflagellate Karenia mikimotoi |
title_full |
Seawater carbonate chemistry and physiology and toxicity of the dinoflagellate Karenia mikimotoi |
title_fullStr |
Seawater carbonate chemistry and physiology and toxicity of the dinoflagellate Karenia mikimotoi |
title_full_unstemmed |
Seawater carbonate chemistry and physiology and toxicity of the dinoflagellate Karenia mikimotoi |
title_sort |
seawater carbonate chemistry and physiology and toxicity of the dinoflagellate karenia mikimotoi |
publisher |
PANGAEA |
publishDate |
2019 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.907178 https://doi.org/10.1594/PANGAEA.907178 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Supplement to: Wang, Hong; Niu, Xiaoqin; Feng, Xinqian; Gonçalves, Rodrigo J; Guan, WanChun (2019): Effects of ocean acidification and phosphate limitation on physiology and toxicity of the dinoflagellate Karenia mikimotoi. Harmful Algae, 87, 101621, https://doi.org/10.1016/j.hal.2019.101621 |
op_relation |
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.907178 https://doi.org/10.1594/PANGAEA.907178 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.90717810.1016/j.hal.2019.101621 |
_version_ |
1810469199762423808 |