Seawater carbonate chemistry and growth and chlorophyll, photochemical parameters, carbon fixation of diatom Phaeodactylum tricornutum
Elevated CO2 is leading to a decrease in pH in marine environments (ocean acidification [OA]), altering marine carbonate chemistry. OA can influence the metabolism of many marine organisms; however, no consensus has been reached on its effects on algal photosynthetic carbon fixation and primary prod...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.900658 2024-09-15T18:28:11+00:00 Seawater carbonate chemistry and growth and chlorophyll, photochemical parameters, carbon fixation of diatom Phaeodactylum tricornutum Liu, Nana Beardall, John Gao, Kunshan 2017 text/tab-separated-values, 6177 data points https://doi.pangaea.de/10.1594/PANGAEA.900658 https://doi.org/10.1594/PANGAEA.900658 en eng PANGAEA Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.900658 https://doi.org/10.1594/PANGAEA.900658 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Liu, Nana; Beardall, John; Gao, Kunshan (2017): Elevated CO2 and associated seawater chemistry do not benefit a model diatom grown with increased availability of light. Aquatic Microbial Ecology, 79(2), 137-147, https://doi.org/10.3354/ame01820 Alkalinity total Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved intracellular pool Carbonate ion Carbonate system computation flag Carbon dioxide Chromista Cumulative carbon fixation per cell Effective quantum yield Factor Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate Identification Initial slope of photosynthesis/dissolved inorganic carbon Laboratory experiment Laboratory strains Light Light capturing capacity Light saturated maximum photosynthetic rate per cell Light saturation point Maximal electron transport rate relative Maximum photochemical quantum yield of photosystem II Not applicable OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Phaeodactylum tricornutum Phytoplankton Primary production/Photosynthesis Registration number of species Salinity Single species Species Temperature dataset 2017 ftpangaea https://doi.org/10.1594/PANGAEA.90065810.3354/ame01820 2024-07-24T02:31:34Z Elevated CO2 is leading to a decrease in pH in marine environments (ocean acidification [OA]), altering marine carbonate chemistry. OA can influence the metabolism of many marine organisms; however, no consensus has been reached on its effects on algal photosynthetic carbon fixation and primary production. Here, we found that when the diatom Phaeodactylum tricornutum was grown under different pCO2 levels, it showed different responses to elevated pCO2 levels under growth-limiting (20 µmol photons/m**2/s, LL) compared with growth-saturating (200 µmol photons/m**2/s, HL) light levels. With pCO2 increased up to 950 µatm, growth rates and primary productivity increased, but in the HL cells, these parameters decreased significantly at higher concentrations up to 5000 µatm, while no difference in growth was observed with pCO2 for the LL cells. Elevated CO2 concentrations reduced the size of the intracellular dissolved inorganic carbon (DIC) pool by 81% and 60% under the LL and HL levels, respectively, with the corresponding photosynthetic affinity for DIC decreasing by 48% and 55%. Little photoinhibition was observed across all treatments. These results suggest that the decreased growth rates under higher CO2 levels in the HL cells were most likely due to acid stress. Low energy demand of growth and energy saving from the down-regulation of the CO2 concentrating mechanisms (CCM) minimized the effects of acid stress on the growth of the LL cells. These findings imply that OA treatment, except for down-regulating CCM, caused stress on the diatom, reflected in diminished C assimilation and growth rates. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved intracellular pool Carbonate ion Carbonate system computation flag Carbon dioxide Chromista Cumulative carbon fixation per cell Effective quantum yield Factor Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate Identification Initial slope of photosynthesis/dissolved inorganic carbon Laboratory experiment Laboratory strains Light Light capturing capacity Light saturated maximum photosynthetic rate per cell Light saturation point Maximal electron transport rate relative Maximum photochemical quantum yield of photosystem II Not applicable OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Phaeodactylum tricornutum Phytoplankton Primary production/Photosynthesis Registration number of species Salinity Single species Species Temperature |
spellingShingle |
Alkalinity total Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved intracellular pool Carbonate ion Carbonate system computation flag Carbon dioxide Chromista Cumulative carbon fixation per cell Effective quantum yield Factor Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate Identification Initial slope of photosynthesis/dissolved inorganic carbon Laboratory experiment Laboratory strains Light Light capturing capacity Light saturated maximum photosynthetic rate per cell Light saturation point Maximal electron transport rate relative Maximum photochemical quantum yield of photosystem II Not applicable OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Phaeodactylum tricornutum Phytoplankton Primary production/Photosynthesis Registration number of species Salinity Single species Species Temperature Liu, Nana Beardall, John Gao, Kunshan Seawater carbonate chemistry and growth and chlorophyll, photochemical parameters, carbon fixation of diatom Phaeodactylum tricornutum |
topic_facet |
Alkalinity total Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved intracellular pool Carbonate ion Carbonate system computation flag Carbon dioxide Chromista Cumulative carbon fixation per cell Effective quantum yield Factor Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate Identification Initial slope of photosynthesis/dissolved inorganic carbon Laboratory experiment Laboratory strains Light Light capturing capacity Light saturated maximum photosynthetic rate per cell Light saturation point Maximal electron transport rate relative Maximum photochemical quantum yield of photosystem II Not applicable OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Phaeodactylum tricornutum Phytoplankton Primary production/Photosynthesis Registration number of species Salinity Single species Species Temperature |
description |
Elevated CO2 is leading to a decrease in pH in marine environments (ocean acidification [OA]), altering marine carbonate chemistry. OA can influence the metabolism of many marine organisms; however, no consensus has been reached on its effects on algal photosynthetic carbon fixation and primary production. Here, we found that when the diatom Phaeodactylum tricornutum was grown under different pCO2 levels, it showed different responses to elevated pCO2 levels under growth-limiting (20 µmol photons/m**2/s, LL) compared with growth-saturating (200 µmol photons/m**2/s, HL) light levels. With pCO2 increased up to 950 µatm, growth rates and primary productivity increased, but in the HL cells, these parameters decreased significantly at higher concentrations up to 5000 µatm, while no difference in growth was observed with pCO2 for the LL cells. Elevated CO2 concentrations reduced the size of the intracellular dissolved inorganic carbon (DIC) pool by 81% and 60% under the LL and HL levels, respectively, with the corresponding photosynthetic affinity for DIC decreasing by 48% and 55%. Little photoinhibition was observed across all treatments. These results suggest that the decreased growth rates under higher CO2 levels in the HL cells were most likely due to acid stress. Low energy demand of growth and energy saving from the down-regulation of the CO2 concentrating mechanisms (CCM) minimized the effects of acid stress on the growth of the LL cells. These findings imply that OA treatment, except for down-regulating CCM, caused stress on the diatom, reflected in diminished C assimilation and growth rates. |
format |
Dataset |
author |
Liu, Nana Beardall, John Gao, Kunshan |
author_facet |
Liu, Nana Beardall, John Gao, Kunshan |
author_sort |
Liu, Nana |
title |
Seawater carbonate chemistry and growth and chlorophyll, photochemical parameters, carbon fixation of diatom Phaeodactylum tricornutum |
title_short |
Seawater carbonate chemistry and growth and chlorophyll, photochemical parameters, carbon fixation of diatom Phaeodactylum tricornutum |
title_full |
Seawater carbonate chemistry and growth and chlorophyll, photochemical parameters, carbon fixation of diatom Phaeodactylum tricornutum |
title_fullStr |
Seawater carbonate chemistry and growth and chlorophyll, photochemical parameters, carbon fixation of diatom Phaeodactylum tricornutum |
title_full_unstemmed |
Seawater carbonate chemistry and growth and chlorophyll, photochemical parameters, carbon fixation of diatom Phaeodactylum tricornutum |
title_sort |
seawater carbonate chemistry and growth and chlorophyll, photochemical parameters, carbon fixation of diatom phaeodactylum tricornutum |
publisher |
PANGAEA |
publishDate |
2017 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.900658 https://doi.org/10.1594/PANGAEA.900658 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Supplement to: Liu, Nana; Beardall, John; Gao, Kunshan (2017): Elevated CO2 and associated seawater chemistry do not benefit a model diatom grown with increased availability of light. Aquatic Microbial Ecology, 79(2), 137-147, https://doi.org/10.3354/ame01820 |
op_relation |
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.900658 https://doi.org/10.1594/PANGAEA.900658 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.90065810.3354/ame01820 |
_version_ |
1810469514538647552 |