Extracellular enzymatic activity from the Arctic Ocean during POLARSTERN cruise ARK-XXVII/3 (IceArc) from August-September 2012
Sampling was conducted with a TV-guided multiple corer (MUC), in order to retrieve undisturbed sediment cores. Upon retrieval of the MUC, the overlying water was carefully removed from each core and the core was cut into the following sediment horizons using a steel plate and a custom-made plastic r...
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Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.892284 https://doi.org/10.1594/PANGAEA.892284 |
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.892284 2023-05-15T14:27:47+02:00 Extracellular enzymatic activity from the Arctic Ocean during POLARSTERN cruise ARK-XXVII/3 (IceArc) from August-September 2012 Bienhold, Christina Boetius, Antje MEDIAN LATITUDE: 78.147256 * MEDIAN LONGITUDE: 124.209741 * SOUTH-BOUND LATITUDE: 77.169170 * WEST-BOUND LONGITUDE: 114.916000 * NORTH-BOUND LATITUDE: 79.649820 * EAST-BOUND LONGITUDE: 136.967680 * DATE/TIME START: 2012-08-28T21:22:00 * DATE/TIME END: 2012-09-02T20:39:00 * MINIMUM DEPTH, sediment/rock: 0.005 m * MAXIMUM DEPTH, sediment/rock: 0.075 m 2018-07-09 text/tab-separated-values, 147 data points https://doi.pangaea.de/10.1594/PANGAEA.892284 https://doi.org/10.1594/PANGAEA.892284 en eng PANGAEA Bienhold, Christina (2021): Benthic bacterial diversity along the Laptev Sea continental slope in the Arctic Ocean between two contrasting years (1993 and 2012). insdc:PRJEB43433 Bienhold, Christina; Schourup-Kristensen, Vibe; Krumpen, Thomas; Nöthig, Eva-Maria; Wenzhöfer, Frank; Korhonen, Meri; Vredenborg, Myriel; Hehemann, Laura; Boetius, Antje (2022): Effects of sea ice retreat and ocean warming on the Laptev Sea continental slope ecosystem (1993 vs 2012). Frontiers in Marine Science, 9, 1004959, https://doi.org/10.3389/fmars.2022.1004959 https://doi.pangaea.de/10.1594/PANGAEA.892284 https://doi.org/10.1594/PANGAEA.892284 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven ABYSS Aminopeptidase activity Arctic Ocean ARK-XXVII/3 Assessment of bacterial life and matter cycling in deep-sea surface sediments beta-glucosidase activity Chitobiase activity DEPTH sediment/rock Esterase activity per sediment volume Event label Fluorescence spectrometer (Hitachi F2000) Laptev Sea Multicorer with television Polarstern PS80/292-1 PS80/296-1 PS80/299-1 PS80/302-1 PS80/309-1 PS80/310-1 PS80/312-1 PS80/313-1 PS80/318-1 PS80/320-1 PS80 IceArc Sample comment TVMUC Dataset 2018 ftpangaea https://doi.org/10.1594/PANGAEA.892284 https://doi.org/10.3389/fmars.2022.1004959 2023-01-20T09:11:18Z Sampling was conducted with a TV-guided multiple corer (MUC), in order to retrieve undisturbed sediment cores. Upon retrieval of the MUC, the overlying water was carefully removed from each core and the core was cut into the following sediment horizons using a steel plate and a custom-made plastic ring: 0-1 cm, 1-5 cm, 5-10 cm. At each station, sediment samples from three replicate cores were pooled and transferred to a cooling container (0°C), where subsamples for extracellular enzymatic activity analyses were taken. The potential extracellular enzymatic activities of the hydrolases beta-glucosidase, chitobiase, aminopeptidase and extracellular enzymes acting as esterases were determined in alignment with Boetius and Lochte, 1994 (https://doi.org/10.3354/MEPS104299) and Boetius & Damm 1998 (https://doi.org/10.1016/S0967-0637(97)00052-6). The method uses small substrate proxies linked to a fluorophore. When the fluorophore is cleaved from the substrate, enzymatic hydrolysis is measured as an increase in fluorescence with time. Determinations of extracellular enzymatic activities were performed in triplicate for all samples with a Hitachi F-2000 spectrofluorometer. Dataset Arctic Arctic Arctic Ocean laptev Laptev Sea PANGAEA - Data Publisher for Earth & Environmental Science Arctic Arctic Ocean Laptev Sea Damm ENVELOPE(162.617,162.617,-82.600,-82.600) ENVELOPE(114.916000,136.967680,79.649820,77.169170) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
ABYSS Aminopeptidase activity Arctic Ocean ARK-XXVII/3 Assessment of bacterial life and matter cycling in deep-sea surface sediments beta-glucosidase activity Chitobiase activity DEPTH sediment/rock Esterase activity per sediment volume Event label Fluorescence spectrometer (Hitachi F2000) Laptev Sea Multicorer with television Polarstern PS80/292-1 PS80/296-1 PS80/299-1 PS80/302-1 PS80/309-1 PS80/310-1 PS80/312-1 PS80/313-1 PS80/318-1 PS80/320-1 PS80 IceArc Sample comment TVMUC |
spellingShingle |
ABYSS Aminopeptidase activity Arctic Ocean ARK-XXVII/3 Assessment of bacterial life and matter cycling in deep-sea surface sediments beta-glucosidase activity Chitobiase activity DEPTH sediment/rock Esterase activity per sediment volume Event label Fluorescence spectrometer (Hitachi F2000) Laptev Sea Multicorer with television Polarstern PS80/292-1 PS80/296-1 PS80/299-1 PS80/302-1 PS80/309-1 PS80/310-1 PS80/312-1 PS80/313-1 PS80/318-1 PS80/320-1 PS80 IceArc Sample comment TVMUC Bienhold, Christina Boetius, Antje Extracellular enzymatic activity from the Arctic Ocean during POLARSTERN cruise ARK-XXVII/3 (IceArc) from August-September 2012 |
topic_facet |
ABYSS Aminopeptidase activity Arctic Ocean ARK-XXVII/3 Assessment of bacterial life and matter cycling in deep-sea surface sediments beta-glucosidase activity Chitobiase activity DEPTH sediment/rock Esterase activity per sediment volume Event label Fluorescence spectrometer (Hitachi F2000) Laptev Sea Multicorer with television Polarstern PS80/292-1 PS80/296-1 PS80/299-1 PS80/302-1 PS80/309-1 PS80/310-1 PS80/312-1 PS80/313-1 PS80/318-1 PS80/320-1 PS80 IceArc Sample comment TVMUC |
description |
Sampling was conducted with a TV-guided multiple corer (MUC), in order to retrieve undisturbed sediment cores. Upon retrieval of the MUC, the overlying water was carefully removed from each core and the core was cut into the following sediment horizons using a steel plate and a custom-made plastic ring: 0-1 cm, 1-5 cm, 5-10 cm. At each station, sediment samples from three replicate cores were pooled and transferred to a cooling container (0°C), where subsamples for extracellular enzymatic activity analyses were taken. The potential extracellular enzymatic activities of the hydrolases beta-glucosidase, chitobiase, aminopeptidase and extracellular enzymes acting as esterases were determined in alignment with Boetius and Lochte, 1994 (https://doi.org/10.3354/MEPS104299) and Boetius & Damm 1998 (https://doi.org/10.1016/S0967-0637(97)00052-6). The method uses small substrate proxies linked to a fluorophore. When the fluorophore is cleaved from the substrate, enzymatic hydrolysis is measured as an increase in fluorescence with time. Determinations of extracellular enzymatic activities were performed in triplicate for all samples with a Hitachi F-2000 spectrofluorometer. |
format |
Dataset |
author |
Bienhold, Christina Boetius, Antje |
author_facet |
Bienhold, Christina Boetius, Antje |
author_sort |
Bienhold, Christina |
title |
Extracellular enzymatic activity from the Arctic Ocean during POLARSTERN cruise ARK-XXVII/3 (IceArc) from August-September 2012 |
title_short |
Extracellular enzymatic activity from the Arctic Ocean during POLARSTERN cruise ARK-XXVII/3 (IceArc) from August-September 2012 |
title_full |
Extracellular enzymatic activity from the Arctic Ocean during POLARSTERN cruise ARK-XXVII/3 (IceArc) from August-September 2012 |
title_fullStr |
Extracellular enzymatic activity from the Arctic Ocean during POLARSTERN cruise ARK-XXVII/3 (IceArc) from August-September 2012 |
title_full_unstemmed |
Extracellular enzymatic activity from the Arctic Ocean during POLARSTERN cruise ARK-XXVII/3 (IceArc) from August-September 2012 |
title_sort |
extracellular enzymatic activity from the arctic ocean during polarstern cruise ark-xxvii/3 (icearc) from august-september 2012 |
publisher |
PANGAEA |
publishDate |
2018 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.892284 https://doi.org/10.1594/PANGAEA.892284 |
op_coverage |
MEDIAN LATITUDE: 78.147256 * MEDIAN LONGITUDE: 124.209741 * SOUTH-BOUND LATITUDE: 77.169170 * WEST-BOUND LONGITUDE: 114.916000 * NORTH-BOUND LATITUDE: 79.649820 * EAST-BOUND LONGITUDE: 136.967680 * DATE/TIME START: 2012-08-28T21:22:00 * DATE/TIME END: 2012-09-02T20:39:00 * MINIMUM DEPTH, sediment/rock: 0.005 m * MAXIMUM DEPTH, sediment/rock: 0.075 m |
long_lat |
ENVELOPE(162.617,162.617,-82.600,-82.600) ENVELOPE(114.916000,136.967680,79.649820,77.169170) |
geographic |
Arctic Arctic Ocean Laptev Sea Damm |
geographic_facet |
Arctic Arctic Ocean Laptev Sea Damm |
genre |
Arctic Arctic Arctic Ocean laptev Laptev Sea |
genre_facet |
Arctic Arctic Arctic Ocean laptev Laptev Sea |
op_source |
Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven |
op_relation |
Bienhold, Christina (2021): Benthic bacterial diversity along the Laptev Sea continental slope in the Arctic Ocean between two contrasting years (1993 and 2012). insdc:PRJEB43433 Bienhold, Christina; Schourup-Kristensen, Vibe; Krumpen, Thomas; Nöthig, Eva-Maria; Wenzhöfer, Frank; Korhonen, Meri; Vredenborg, Myriel; Hehemann, Laura; Boetius, Antje (2022): Effects of sea ice retreat and ocean warming on the Laptev Sea continental slope ecosystem (1993 vs 2012). Frontiers in Marine Science, 9, 1004959, https://doi.org/10.3389/fmars.2022.1004959 https://doi.pangaea.de/10.1594/PANGAEA.892284 https://doi.org/10.1594/PANGAEA.892284 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.892284 https://doi.org/10.3389/fmars.2022.1004959 |
_version_ |
1766301703984906240 |