Seawater carbonate chemistry and gene abundance and community composition in two contrasting coastal marine sediments
Marine ecosystems are exposed to a range of human-induced climate stressors, in particular changing carbonate chemistry and elevated sea surface temperatures as a consequence of climate change. More research effort is needed to reduce uncertainties about the effects of global-scale warming and acidi...
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Language: | English |
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PANGAEA
2017
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Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.890872 https://doi.org/10.1594/PANGAEA.890872 |
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.890872 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total standard deviation Ammonium Aragonite saturation state Benthos Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Campaign Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Community composition and diversity Containers and aquaria (20-1000 L or < 1 m**2) Day of experiment Depth description Eden_Estuary Entire community Evenness of species Event label EXP Experiment Experiment duration Experiment week Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene abundance Identification Laboratory experiment Margelf index Nitrate and Nitrite North Atlantic OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) |
spellingShingle |
Alkalinity total standard deviation Ammonium Aragonite saturation state Benthos Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Campaign Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Community composition and diversity Containers and aquaria (20-1000 L or < 1 m**2) Day of experiment Depth description Eden_Estuary Entire community Evenness of species Event label EXP Experiment Experiment duration Experiment week Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene abundance Identification Laboratory experiment Margelf index Nitrate and Nitrite North Atlantic OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Currie, Ashleigh R Tait, Karen Parry, Helen E de Francisco-Mora, Beatriz Hicks, Natalie Osborn, A M Widdicombe, Steve Stahl, Henrik Seawater carbonate chemistry and gene abundance and community composition in two contrasting coastal marine sediments |
topic_facet |
Alkalinity total standard deviation Ammonium Aragonite saturation state Benthos Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Campaign Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Community composition and diversity Containers and aquaria (20-1000 L or < 1 m**2) Day of experiment Depth description Eden_Estuary Entire community Evenness of species Event label EXP Experiment Experiment duration Experiment week Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene abundance Identification Laboratory experiment Margelf index Nitrate and Nitrite North Atlantic OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) |
description |
Marine ecosystems are exposed to a range of human-induced climate stressors, in particular changing carbonate chemistry and elevated sea surface temperatures as a consequence of climate change. More research effort is needed to reduce uncertainties about the effects of global-scale warming and acidification for benthic microbial communities, which drive sedimentary biogeochemical cycles. In this research, mesocosm experiments were set up using muddy and sandy coastal sediments to investigate the independent and interactive effects of elevated carbon dioxide concentrations (750 ppm CO2) and elevated temperature (ambient + 4 °C) on the abundance of taxonomic and functional microbial genes. Specific q-PCR primers were used to target archaeal, bacterial and cyanobacterial/chloroplast 16S rRNA in both sediment types. Nitrogen cycling genes archaeal and bacterial ammonia monooxygenase (amoA) and bacterial nitrite reductase (nirS) were specifically targeted to identify changes in microbial gene abundance and potential impacts on nitrogen cycling. In muddy sediment, microbial gene abundance, including amoA and nirS genes, increased under elevated temperature and reduced under elevated CO2 after 28 days, accompanied by shifts in community composition. In contrast, the combined stressor treatment showed a non-additive effect with lower microbial gene abundance throughout the experiment. The response of microbial communities in the sandy sediment was less pronounced, with the most noticeable response seen in the archaeal gene abundances in response to environmental stressors over time. 16S rRNA genes (amoA and nirS) were lower in abundance in the combined stressor treatments in sandy sediments. Our results indicated that marine benthic microorganisms, especially in muddy sediments, are susceptible to changes in ocean carbonate chemistry and seawater temperature, which ultimately may have an impact upon key benthic biogeochemical cycles. |
format |
Dataset |
author |
Currie, Ashleigh R Tait, Karen Parry, Helen E de Francisco-Mora, Beatriz Hicks, Natalie Osborn, A M Widdicombe, Steve Stahl, Henrik |
author_facet |
Currie, Ashleigh R Tait, Karen Parry, Helen E de Francisco-Mora, Beatriz Hicks, Natalie Osborn, A M Widdicombe, Steve Stahl, Henrik |
author_sort |
Currie, Ashleigh R |
title |
Seawater carbonate chemistry and gene abundance and community composition in two contrasting coastal marine sediments |
title_short |
Seawater carbonate chemistry and gene abundance and community composition in two contrasting coastal marine sediments |
title_full |
Seawater carbonate chemistry and gene abundance and community composition in two contrasting coastal marine sediments |
title_fullStr |
Seawater carbonate chemistry and gene abundance and community composition in two contrasting coastal marine sediments |
title_full_unstemmed |
Seawater carbonate chemistry and gene abundance and community composition in two contrasting coastal marine sediments |
title_sort |
seawater carbonate chemistry and gene abundance and community composition in two contrasting coastal marine sediments |
publisher |
PANGAEA |
publishDate |
2017 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.890872 https://doi.org/10.1594/PANGAEA.890872 |
op_coverage |
MEDIAN LATITUDE: 56.365835 * MEDIAN LONGITUDE: -2.832360 * SOUTH-BOUND LATITUDE: 56.365000 * WEST-BOUND LONGITUDE: -2.848050 * NORTH-BOUND LATITUDE: 56.366670 * EAST-BOUND LONGITUDE: -2.816670 * DATE/TIME START: 2012-03-12T00:00:00 * DATE/TIME END: 2012-07-23T00:00:00 |
long_lat |
ENVELOPE(-2.848050,-2.816670,56.366670,56.365000) |
genre |
North Atlantic Ocean acidification |
genre_facet |
North Atlantic Ocean acidification |
op_source |
Supplement to: Currie, Ashleigh R; Tait, Karen; Parry, Helen E; de Francisco-Mora, Beatriz; Hicks, Natalie; Osborn, A M; Widdicombe, Steve; Stahl, Henrik (2017): Marine Microbial Gene Abundance and Community Composition in Response to Ocean Acidification and Elevated Temperature in Two Contrasting Coastal Marine Sediments. Frontiers in Microbiology, 8, https://doi.org/10.3389/fmicb.2017.01599 |
op_relation |
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.890872 https://doi.org/10.1594/PANGAEA.890872 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.89087210.3389/fmicb.2017.01599 |
_version_ |
1810464888433147904 |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.890872 2024-09-15T18:24:31+00:00 Seawater carbonate chemistry and gene abundance and community composition in two contrasting coastal marine sediments Currie, Ashleigh R Tait, Karen Parry, Helen E de Francisco-Mora, Beatriz Hicks, Natalie Osborn, A M Widdicombe, Steve Stahl, Henrik MEDIAN LATITUDE: 56.365835 * MEDIAN LONGITUDE: -2.832360 * SOUTH-BOUND LATITUDE: 56.365000 * WEST-BOUND LONGITUDE: -2.848050 * NORTH-BOUND LATITUDE: 56.366670 * EAST-BOUND LONGITUDE: -2.816670 * DATE/TIME START: 2012-03-12T00:00:00 * DATE/TIME END: 2012-07-23T00:00:00 2017 text/tab-separated-values, 16753 data points https://doi.pangaea.de/10.1594/PANGAEA.890872 https://doi.org/10.1594/PANGAEA.890872 en eng PANGAEA Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.890872 https://doi.org/10.1594/PANGAEA.890872 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Currie, Ashleigh R; Tait, Karen; Parry, Helen E; de Francisco-Mora, Beatriz; Hicks, Natalie; Osborn, A M; Widdicombe, Steve; Stahl, Henrik (2017): Marine Microbial Gene Abundance and Community Composition in Response to Ocean Acidification and Elevated Temperature in Two Contrasting Coastal Marine Sediments. Frontiers in Microbiology, 8, https://doi.org/10.3389/fmicb.2017.01599 Alkalinity total standard deviation Ammonium Aragonite saturation state Benthos Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Campaign Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Community composition and diversity Containers and aquaria (20-1000 L or < 1 m**2) Day of experiment Depth description Eden_Estuary Entire community Evenness of species Event label EXP Experiment Experiment duration Experiment week Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene abundance Identification Laboratory experiment Margelf index Nitrate and Nitrite North Atlantic OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) dataset 2017 ftpangaea https://doi.org/10.1594/PANGAEA.89087210.3389/fmicb.2017.01599 2024-07-24T02:31:33Z Marine ecosystems are exposed to a range of human-induced climate stressors, in particular changing carbonate chemistry and elevated sea surface temperatures as a consequence of climate change. More research effort is needed to reduce uncertainties about the effects of global-scale warming and acidification for benthic microbial communities, which drive sedimentary biogeochemical cycles. In this research, mesocosm experiments were set up using muddy and sandy coastal sediments to investigate the independent and interactive effects of elevated carbon dioxide concentrations (750 ppm CO2) and elevated temperature (ambient + 4 °C) on the abundance of taxonomic and functional microbial genes. Specific q-PCR primers were used to target archaeal, bacterial and cyanobacterial/chloroplast 16S rRNA in both sediment types. Nitrogen cycling genes archaeal and bacterial ammonia monooxygenase (amoA) and bacterial nitrite reductase (nirS) were specifically targeted to identify changes in microbial gene abundance and potential impacts on nitrogen cycling. In muddy sediment, microbial gene abundance, including amoA and nirS genes, increased under elevated temperature and reduced under elevated CO2 after 28 days, accompanied by shifts in community composition. In contrast, the combined stressor treatment showed a non-additive effect with lower microbial gene abundance throughout the experiment. The response of microbial communities in the sandy sediment was less pronounced, with the most noticeable response seen in the archaeal gene abundances in response to environmental stressors over time. 16S rRNA genes (amoA and nirS) were lower in abundance in the combined stressor treatments in sandy sediments. Our results indicated that marine benthic microorganisms, especially in muddy sediments, are susceptible to changes in ocean carbonate chemistry and seawater temperature, which ultimately may have an impact upon key benthic biogeochemical cycles. Dataset North Atlantic Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science ENVELOPE(-2.848050,-2.816670,56.366670,56.365000) |