Seawater carbonate chemistry and photophysiology and hemolytic activity of the dinoflagellate Akashiwo sanguinea

• Main Authors: Ou, Guanyong, Wang, Hong, Si, Ranran, Guan, WanChun
• Format: Dataset
• Language: English
• Published: PANGAEA 2017
• Subjects: Akashiwo sanguinea; Alkalinity; total; Aragonite saturation state; Bicarbonate ion; standard deviation; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon; inorganic; dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon fixation rate; per chlorophyll a; Carotenoids; Carotenoids per cell; Chlorophyll a; Chlorophyll a per cell; Chromista; Coast and continental shelf; Dongtou_Island; EXP; Experiment; Experiment duration; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Haemolytic activity; Irradiance; Ocean acidification
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.889140; https://doi.org/10.1594/PANGAEA.889140

Due to global climate change, marine phytoplankton will likely experience low pH (ocean acidification), high temperatures and high irradiance in the future. Here, this work report the results of a batch culture experiment conducted to study the interactive effects of elevated CO2, increased temperature and high irradiance on the harmful dinoflagellate Akashiwo sanguinea, isolated at Dongtou Island, Eastern China Sea. The A. sanguineacells were acclimated in high CO2 condition for about three months before testing the responses of cells to a full factorial matrix experimentation during a 7-day period. This study measured the variation in physiological parameters and hemolytic activity in 8 treatments, representing full factorial combinations of 2 levels each of exposure to CO2(400 and 1000 μatm), temperature (20 and 28 °C) and irradiance (50 and 200 μmol photons /m**2/s). Sustained growth of A. sanguinea occurred in all treatments, but high CO2 (HC) stimulated faster growth than low CO2 (LC). The pigments (chlorophyll a and carotenoid) decreased in all HC treatments. The quantum yield (Fv/Fm) declined slightly in all high-temperature (HT) treatments. High irradiance (HL) induced the accumulation of ultraviolet-absorbing compounds (UVabc) irrespective of temperature and CO2. The hemolytic activity in the LC treatments, however, declined when exposed to HT and HL, but HC alleviated the adverse effects of HT and HL on hemolytic activity. All HC and HL conditions and the combinations of high temperature*high light (HTHL) and high CO2*high temperature*high light (HCHTHL) positively affected the growth in comparison to the low CO2*low temperature*low light (LCLTLL) treatment. High temperature (HT), high light (HL) and a combination of HT*HL, however, negatively impacted hemolytic activity. CO2 was the main factor that affected the growth and hemolytic activity. There were no significant interactive effects of CO2*temperature*irradiance on growth, pigment, Fv/Fm or hemolytic activity, but there were effects on Pm, α, ...