Nitrogen and carbon processes in the South Indian Ocean and the French Southern and Antarctic Lands
Our data, as part of the OISO (Ocean Indien Service d'Observation) campaign, contributes to a better understanding of the physical and biological factors controlling N2 fixation in the Southern Indian Ocean and the French Southern and Antarctic lands during Austral summer January and February 2...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.885896 2023-05-15T14:04:56+02:00 Nitrogen and carbon processes in the South Indian Ocean and the French Southern and Antarctic Lands Hörstmann, Cora Raes, Eric J Waite, Anya M MEDIAN LATITUDE: -44.448773 * MEDIAN LONGITUDE: 64.032235 * SOUTH-BOUND LATITUDE: -56.498500 * WEST-BOUND LONGITUDE: 52.002500 * NORTH-BOUND LATITUDE: -27.999830 * EAST-BOUND LONGITUDE: 76.406830 * DATE/TIME START: 2017-01-06T17:25:00 * DATE/TIME END: 2017-02-06T10:15:00 2018-02-02 application/zip, 4 datasets https://doi.pangaea.de/10.1594/PANGAEA.885896 https://doi.org/10.1594/PANGAEA.885896 en eng PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.885896 https://doi.org/10.1594/PANGAEA.885896 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Dataset 2018 ftpangaea https://doi.org/10.1594/PANGAEA.885896 2023-01-20T07:34:06Z Our data, as part of the OISO (Ocean Indien Service d'Observation) campaign, contributes to a better understanding of the physical and biological factors controlling N2 fixation in the Southern Indian Ocean and the French Southern and Antarctic lands during Austral summer January and February 2017. We measured N2 and C fixation as well as NH4+ and NO3- assimilation in 3-6 replicates per station. Additionally, we measured diagnostic pigment concentrations to evaluate phtosynthetic community composition. For pigment analysis 4L water was filtered through 25mm Whatman GF/F filters (pressure drop <10kPa). Samples were stored at -80°C until analysis. Pigments were analysed using High Performance Liquid Chromatography (HPLC). Pigment concentration were calculated according to Kilias et al (2013, doi:10.1111/jpy.12109). N2 fixation experiments were carried out in three to six replicates for each station. Incubations were done in pre-acid washed polycarbonate bottles on deck with ambient light conditions. All polycarbonate incubation bottles were rinsed with deionized water, and seawater prior to incubation. We used the combination of the bubble approach (Montoya et al., 1996) and the dissolution method (Mohr et al., 2010, doi:10.1371/journal.pone.0012583) proposed by Klawonn et al. (2015, doi:10.3389/fmicb.2015.00769). Bottles were filled up to capacity to avoid air contamination. Incubations were initialized by adding a 10 ml 15-15N gas bubble. Bottles were gently rocked for 15 minutes. Finally, the remaining bubble was removed to avoid equilibration between gas and aqueous phase. after 24 hours a water subsample was taken to a 12 ml exetainer and preserved with 100 µl HgCl2 solution for later determination of exact 15N-15N concentration. Natural 15N2 was determined using Membrane Inlet Mass Spectrometry (MIMS; GAM200, IPI) for each station. Analysis of 15N2 incorporated was carried out by the Isotopic Laboratory at the UC Davis, California campus. We used stable isotope tracers (15N) to measure dissolved inorganic ... Dataset Antarc* Antarctic PANGAEA - Data Publisher for Earth & Environmental Science Antarctic Austral Indian ENVELOPE(52.002500,76.406830,-27.999830,-56.498500) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
description |
Our data, as part of the OISO (Ocean Indien Service d'Observation) campaign, contributes to a better understanding of the physical and biological factors controlling N2 fixation in the Southern Indian Ocean and the French Southern and Antarctic lands during Austral summer January and February 2017. We measured N2 and C fixation as well as NH4+ and NO3- assimilation in 3-6 replicates per station. Additionally, we measured diagnostic pigment concentrations to evaluate phtosynthetic community composition. For pigment analysis 4L water was filtered through 25mm Whatman GF/F filters (pressure drop <10kPa). Samples were stored at -80°C until analysis. Pigments were analysed using High Performance Liquid Chromatography (HPLC). Pigment concentration were calculated according to Kilias et al (2013, doi:10.1111/jpy.12109). N2 fixation experiments were carried out in three to six replicates for each station. Incubations were done in pre-acid washed polycarbonate bottles on deck with ambient light conditions. All polycarbonate incubation bottles were rinsed with deionized water, and seawater prior to incubation. We used the combination of the bubble approach (Montoya et al., 1996) and the dissolution method (Mohr et al., 2010, doi:10.1371/journal.pone.0012583) proposed by Klawonn et al. (2015, doi:10.3389/fmicb.2015.00769). Bottles were filled up to capacity to avoid air contamination. Incubations were initialized by adding a 10 ml 15-15N gas bubble. Bottles were gently rocked for 15 minutes. Finally, the remaining bubble was removed to avoid equilibration between gas and aqueous phase. after 24 hours a water subsample was taken to a 12 ml exetainer and preserved with 100 µl HgCl2 solution for later determination of exact 15N-15N concentration. Natural 15N2 was determined using Membrane Inlet Mass Spectrometry (MIMS; GAM200, IPI) for each station. Analysis of 15N2 incorporated was carried out by the Isotopic Laboratory at the UC Davis, California campus. We used stable isotope tracers (15N) to measure dissolved inorganic ... |
format |
Dataset |
author |
Hörstmann, Cora Raes, Eric J Waite, Anya M |
spellingShingle |
Hörstmann, Cora Raes, Eric J Waite, Anya M Nitrogen and carbon processes in the South Indian Ocean and the French Southern and Antarctic Lands |
author_facet |
Hörstmann, Cora Raes, Eric J Waite, Anya M |
author_sort |
Hörstmann, Cora |
title |
Nitrogen and carbon processes in the South Indian Ocean and the French Southern and Antarctic Lands |
title_short |
Nitrogen and carbon processes in the South Indian Ocean and the French Southern and Antarctic Lands |
title_full |
Nitrogen and carbon processes in the South Indian Ocean and the French Southern and Antarctic Lands |
title_fullStr |
Nitrogen and carbon processes in the South Indian Ocean and the French Southern and Antarctic Lands |
title_full_unstemmed |
Nitrogen and carbon processes in the South Indian Ocean and the French Southern and Antarctic Lands |
title_sort |
nitrogen and carbon processes in the south indian ocean and the french southern and antarctic lands |
publisher |
PANGAEA |
publishDate |
2018 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.885896 https://doi.org/10.1594/PANGAEA.885896 |
op_coverage |
MEDIAN LATITUDE: -44.448773 * MEDIAN LONGITUDE: 64.032235 * SOUTH-BOUND LATITUDE: -56.498500 * WEST-BOUND LONGITUDE: 52.002500 * NORTH-BOUND LATITUDE: -27.999830 * EAST-BOUND LONGITUDE: 76.406830 * DATE/TIME START: 2017-01-06T17:25:00 * DATE/TIME END: 2017-02-06T10:15:00 |
long_lat |
ENVELOPE(52.002500,76.406830,-27.999830,-56.498500) |
geographic |
Antarctic Austral Indian |
geographic_facet |
Antarctic Austral Indian |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
https://doi.pangaea.de/10.1594/PANGAEA.885896 https://doi.org/10.1594/PANGAEA.885896 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.885896 |
_version_ |
1766276399181594624 |