Intact polar diacylglycerol (IP-DAG) lipids measured in cultures of four Antarctic diatom isolates

• Main Authors: Collins, James R, Ducklow, Hugh W, Marchetti, Adrian, Van Mooy, Benjamin A S
• Format: Dataset
• Language: English
• Published: PANGAEA 2017
• Subjects: Diacylglyceryl trimethylhomoserine and diacylglyceryl hydroxymethyl-trimethyl-beta-alanine 30:0; Diacylglyceryl trimethylhomoserine and diacylglyceryl hydroxymethyl-trimethyl-beta-alanine 30:1; Diacylglyceryl trimethylhomoserine and diacylglyceryl hydroxymethyl-trimethyl-beta-alanine 32:2; Diacylglyceryl trimethylhomoserine and diacylglyceryl hydroxymethyl-trimethyl-beta-alanine 33:5; Diacylglyceryl trimethylhomoserine and diacylglyceryl hydroxymethyl-trimethyl-beta-alanine 34:5; Diacylglyceryl trimethylhomoserine and diacylglyceryl hydroxymethyl-trimethyl-beta-alanine 36:6; Diacylglyceryl trimethylhomoserine and diacylglyceryl hydroxymethyl-trimethyl-beta-alanine 40:10; Digalactosyldiacylglycerol 30:0; Digalactosyldiacylglycerol 30:1; Digalactosyldiacylglycerol 30:2; Digalactosyldiacylglycerol 30:4; Digalactosyldiacylglycerol 31:3; Digalactosyldiacylglycerol 32:1; Digalactosyldiacylglycerol 32:2; Digalactosyldiacylglycerol 32:3; Digalactosyldiacylglycerol 32:4; Digalactosyldiacylglycerol 32:5; Digalactosyldiacylglycerol 32:6; Digalactosyldiacylglycerol 32:7; Digalactosyldiacylglycerol 34:2; Digalactosyldiacylglycerol 34:3; Digalactosyldiacylglycerol 34:6; Digalactosyldiacylglycerol 34:7; Digalactosyldiacylglycerol 36:2; Digalactosyldiacylglycerol 36:4; Digalactosyldiacylglycerol 36:5; Digalactosyldiacylglycerol 36:6; Digalactosyldiacylglycerol 36:7; Digalactosyldiacylglycerol 36:8; Digalactosyldiacylglycerol 36:9; Digalactosyldiacylglycerol 37:8; Digalactosyldiacylglycerol 38:9; Monitoring station; Monogalactosyldiacylglycerol 30:0; Monogalactosyldiacylglycerol 30:1; Monogalactosyldiacylglycerol 30:2; Monogalactosyldiacylglycerol 30:3; Monogalactosyldiacylglycerol 30:4; Monogalactosyldiacylglycerol 32:0; Monogalactosyldiacylglycerol 32:1; Monogalactosyldiacylglycerol 32:2; Monogalactosyldiacylglycerol 32:3; Monogalactosyldiacylglycerol 32:4; Monogalactosyldiacylglycerol 32:5; Monogalactosyldiacylglycerol 32:6; Monogalactosyldiacylglycerol 32:7; Monogalactosyldiacylglycerol 32:8; Monogalactosyldiacylglycerol 34:0; Monogalactosyldiacylglycerol 34:1; Monogalactosyldiacylglycerol 34:2; Antarctic; Bellingshausen Sea; Dyer; Antarc*
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.879617; https://doi.org/10.1594/PANGAEA.879617

Diatoms were isolated by Adrian Marchetti (orcid:0000-0003-4608-4775) from waters of the Bellingshausen Sea and grown on replete media. Cells were collected onto 0.7 µm GF/F filters. Extraction was performed using a modified Bligh and Dyer (Bligh and Dyer, 1959; https://doi.org/10.1139/o59-099) method described in Popendorf et al. (2013; https://doi.org/10.1007/s11745-012-3748-0). Lipid extracts were analyzed by HPLC-ESI-MS with data dependent-MS2 acquisition on a high-resolution, accurate mass Thermo Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (ThermoFisher Scientific, Waltham, MA, USA) coupled to an Agilent 1200 HPLC system (Agilent, Santa Clara, CA, USA). The HPLC-ESI-MS method is described in Collins et al., 2016 (https://doi.org/10.1021/acs.analchem.6b01260). The LOBSTAHS lipidomics discovery software (Collins et al., 2016; https://doi.org/10.1021/acs.analchem.6b01260) was used to putatively identify HPLC-MS features in the data. We confirmed each LOBSTAHS identification using two additional means: (1) via comparison of data-dependent MS2 spectra with those from authentic standards or published reference spectra and (2) by requiring the presence of the same compound identity in data acquired in the opposite HPLC-ESI-MS ionization mode. We confirmed all LOBSTAHS identities at the lipid class level (e.g., PC versus PE, or MGDG versus TAG) using a new, experimental LOBSTAHS feature which automatically detects diagnostic product ion fragments and constant neutral losses (as given in Popendorf et al., 2013; https://doi.org/10.1007/s11745-012-3748-0) in the available data-dependent MS2 spectra for each sample. After identification, quantification of analytes was performed using a series of standard curves, followed by normalization to concentration of an internal standard. Lipid identities are resolved only to the level of bulk fatty acid composition (i.e., the sum of the properties of the substituents at both the sn-1 and sn-2 positions). As a result, in some cases (e.g., PC 40:8), several different ...