Abundance of Prokaryotes in deep-sea sediments analyzed by CARD-FISH
We fixed 0.5 g aliquots of sediment with a 4% formaldehyde solution for 2-4 h, washed the fixed sediments three times with 1x phosphate-buffered saline (PBS), before storing them in 50% ethanol/PBS at -20°C. For water samples, we fixed 10 ml (for samples from the deep chlorophyll maximum and 100 m w...
Main Authors: | , , , , , , , |
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Format: | Dataset |
Language: | English |
Published: |
PANGAEA
2017
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Subjects: | |
Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.874860 https://doi.org/10.1594/PANGAEA.874860 |