Functional analysis of a tyrosinase gene involved in early larval shell biogenesis in Crassostrea angulata and its response to ocean acidification
The formation of the primary shell is a vital process in marine bivalves. Ocean acidification largely influences shell formation. It has been reported that enzymes involved in phenol oxidation, such as tyrosinase and phenoloxidases, participate in the formation of the periostracum. In the present st...
Main Authors: | , , , , , |
---|---|
Format: | Dataset |
Language: | English |
Published: |
PANGAEA
2017
|
Subjects: | |
Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.874743 https://doi.org/10.1594/PANGAEA.874743 |
id |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.874743 |
---|---|
record_format |
openpolar |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.874743 2024-09-15T18:27:49+00:00 Functional analysis of a tyrosinase gene involved in early larval shell biogenesis in Crassostrea angulata and its response to ocean acidification Yang, Bingye Pu, F Li, lingling You, W Ke, C Feng, Danqing 2017 text/tab-separated-values, 252 data points https://doi.pangaea.de/10.1594/PANGAEA.874743 https://doi.org/10.1594/PANGAEA.874743 en eng PANGAEA Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.874743 https://doi.org/10.1594/PANGAEA.874743 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Yang, Bingye; Pu, F; Li, lingling; You, W; Ke, C; Feng, Danqing (2017): Functional analysis of a tyrosinase gene involved in early larval shell biogenesis in Crassostrea angulata and its response to ocean acidification. Comparative Biochemistry and Physiology Part B: Biochemistry & Molecular Biology, 206, 8-15, https://doi.org/10.1016/j.cbpb.2017.01.006 Alkalinity total Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Crassostrea angulata Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression standard deviation Gene expression (incl. proteomics) Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Registration number of species Salinity Single species Species Stage Temperate Temperature water Treatment Type Uniform resource locator/link to reference Zooplankton dataset 2017 ftpangaea https://doi.org/10.1594/PANGAEA.87474310.1016/j.cbpb.2017.01.006 2024-07-24T02:31:33Z The formation of the primary shell is a vital process in marine bivalves. Ocean acidification largely influences shell formation. It has been reported that enzymes involved in phenol oxidation, such as tyrosinase and phenoloxidases, participate in the formation of the periostracum. In the present study, we cloned a tyrosinase gene from Crassostrea angulata named Ca-tyrA1, and its potential function in early larval shell biogenesis was investigated. The Ca-tyrA1 gene has a full-length cDNA of 2430 bp in size, with an open reading frame of 1896 bp in size, which encodes a 631-amino acid protein that includes a 24-amino acid putative signal peptide. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis revealed that Ca-tyrA1 transcription mainly occurs at the trochophore stage, and the Ca-tyrA1 mRNA levels in the 3000 ppm treatment group were significantly upregulated in the early D-veliger larvae. WMISH and electron scanning microscopy analyses showed that the expression of Ca-tyrA1 occurs at the gastrula stage, thereby sustaining the early D-veliger larvae, and the shape of its signal is saddle-like, similar to that observed under an electron scanning microscope. Furthermore, the RNA interference has shown that the treatment group has a higher deformity rate than that of the control, thereby indicating that Ca-tyrA1 participates in the biogenesis of the primary shell. In conclusion, and our results indicate that Ca-tyrA1 plays a vital role in the formation of the larval shell and participates in the response to larval shell damages in Crassostrea angulata that were induced by ocean acidification. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Crassostrea angulata Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression standard deviation Gene expression (incl. proteomics) Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Registration number of species Salinity Single species Species Stage Temperate Temperature water Treatment Type Uniform resource locator/link to reference Zooplankton |
spellingShingle |
Alkalinity total Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Crassostrea angulata Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression standard deviation Gene expression (incl. proteomics) Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Registration number of species Salinity Single species Species Stage Temperate Temperature water Treatment Type Uniform resource locator/link to reference Zooplankton Yang, Bingye Pu, F Li, lingling You, W Ke, C Feng, Danqing Functional analysis of a tyrosinase gene involved in early larval shell biogenesis in Crassostrea angulata and its response to ocean acidification |
topic_facet |
Alkalinity total Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Crassostrea angulata Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression standard deviation Gene expression (incl. proteomics) Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Registration number of species Salinity Single species Species Stage Temperate Temperature water Treatment Type Uniform resource locator/link to reference Zooplankton |
description |
The formation of the primary shell is a vital process in marine bivalves. Ocean acidification largely influences shell formation. It has been reported that enzymes involved in phenol oxidation, such as tyrosinase and phenoloxidases, participate in the formation of the periostracum. In the present study, we cloned a tyrosinase gene from Crassostrea angulata named Ca-tyrA1, and its potential function in early larval shell biogenesis was investigated. The Ca-tyrA1 gene has a full-length cDNA of 2430 bp in size, with an open reading frame of 1896 bp in size, which encodes a 631-amino acid protein that includes a 24-amino acid putative signal peptide. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis revealed that Ca-tyrA1 transcription mainly occurs at the trochophore stage, and the Ca-tyrA1 mRNA levels in the 3000 ppm treatment group were significantly upregulated in the early D-veliger larvae. WMISH and electron scanning microscopy analyses showed that the expression of Ca-tyrA1 occurs at the gastrula stage, thereby sustaining the early D-veliger larvae, and the shape of its signal is saddle-like, similar to that observed under an electron scanning microscope. Furthermore, the RNA interference has shown that the treatment group has a higher deformity rate than that of the control, thereby indicating that Ca-tyrA1 participates in the biogenesis of the primary shell. In conclusion, and our results indicate that Ca-tyrA1 plays a vital role in the formation of the larval shell and participates in the response to larval shell damages in Crassostrea angulata that were induced by ocean acidification. |
format |
Dataset |
author |
Yang, Bingye Pu, F Li, lingling You, W Ke, C Feng, Danqing |
author_facet |
Yang, Bingye Pu, F Li, lingling You, W Ke, C Feng, Danqing |
author_sort |
Yang, Bingye |
title |
Functional analysis of a tyrosinase gene involved in early larval shell biogenesis in Crassostrea angulata and its response to ocean acidification |
title_short |
Functional analysis of a tyrosinase gene involved in early larval shell biogenesis in Crassostrea angulata and its response to ocean acidification |
title_full |
Functional analysis of a tyrosinase gene involved in early larval shell biogenesis in Crassostrea angulata and its response to ocean acidification |
title_fullStr |
Functional analysis of a tyrosinase gene involved in early larval shell biogenesis in Crassostrea angulata and its response to ocean acidification |
title_full_unstemmed |
Functional analysis of a tyrosinase gene involved in early larval shell biogenesis in Crassostrea angulata and its response to ocean acidification |
title_sort |
functional analysis of a tyrosinase gene involved in early larval shell biogenesis in crassostrea angulata and its response to ocean acidification |
publisher |
PANGAEA |
publishDate |
2017 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.874743 https://doi.org/10.1594/PANGAEA.874743 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Supplement to: Yang, Bingye; Pu, F; Li, lingling; You, W; Ke, C; Feng, Danqing (2017): Functional analysis of a tyrosinase gene involved in early larval shell biogenesis in Crassostrea angulata and its response to ocean acidification. Comparative Biochemistry and Physiology Part B: Biochemistry & Molecular Biology, 206, 8-15, https://doi.org/10.1016/j.cbpb.2017.01.006 |
op_relation |
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.874743 https://doi.org/10.1594/PANGAEA.874743 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.87474310.1016/j.cbpb.2017.01.006 |
_version_ |
1810469081174769664 |