(Supplement 1) Detailed information on environmental samples and sequence data
Deep-sea sediments constitute a unique archive of ocean change, fueled by a permanent rain of mineral and organic remains from the surface ocean. Until now, paleo-ecological analyses of this archive have been mostly based on information from taxa leaving fossils. In theory, environmental DNA (eDNA)...
Main Authors: | , , , , , , , , , |
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Format: | Dataset |
Language: | English |
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PANGAEA
2016
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Subjects: | |
Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.868770 https://doi.org/10.1594/PANGAEA.868770 |
id |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.868770 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
ANT-XXIV/2 Arctic Ocean ARK-XXII/2 AWI_Paleo BC Box corer Center for Marine Environmental Sciences Cruise/expedition Date/Time of event Davis Strait Elevation of event Event label Galathea_3_Win3 Galathea_3_Win4 Galathea_3_Win6 Galathea 3 HDMS Vaedderen KT07-14 KT07-14_MC03 KT07-14_MC04 KT07-14_MC07 Latitude of event Longitude of event Maria S. Merian MARUM MC03 MC04 MC07 MSM09/2 MSM09/2_432-5 MSM09/2_453-7 MSM09/2_472-2 MUC MultiCorer Name North Atlantic Ocean Number of e-ribotype Number of genotype Number of morphospecies Number of sequences Paleoenvironmental Reconstructions from Marine Sediments @ AWI Polarstern PS70/239-6 PS70/265-1 PS70/265-2 PS70/299-2 PS70/309-8 PS70 SPACE DAMOCLES PS71/033-12 PS71/085-5 |
spellingShingle |
ANT-XXIV/2 Arctic Ocean ARK-XXII/2 AWI_Paleo BC Box corer Center for Marine Environmental Sciences Cruise/expedition Date/Time of event Davis Strait Elevation of event Event label Galathea_3_Win3 Galathea_3_Win4 Galathea_3_Win6 Galathea 3 HDMS Vaedderen KT07-14 KT07-14_MC03 KT07-14_MC04 KT07-14_MC07 Latitude of event Longitude of event Maria S. Merian MARUM MC03 MC04 MC07 MSM09/2 MSM09/2_432-5 MSM09/2_453-7 MSM09/2_472-2 MUC MultiCorer Name North Atlantic Ocean Number of e-ribotype Number of genotype Number of morphospecies Number of sequences Paleoenvironmental Reconstructions from Marine Sediments @ AWI Polarstern PS70/239-6 PS70/265-1 PS70/265-2 PS70/299-2 PS70/309-8 PS70 SPACE DAMOCLES PS71/033-12 PS71/085-5 Morard, Raphael Lejzerowicz, Franck Darling, Kate F Lecroq-Bennet, Beatrice Pedersen, Mikkel Winther Orlando, Ludovic Pawlowski, Jan Mulitza, Stefan De Vargas, Colomban Kucera, Michal (Supplement 1) Detailed information on environmental samples and sequence data |
topic_facet |
ANT-XXIV/2 Arctic Ocean ARK-XXII/2 AWI_Paleo BC Box corer Center for Marine Environmental Sciences Cruise/expedition Date/Time of event Davis Strait Elevation of event Event label Galathea_3_Win3 Galathea_3_Win4 Galathea_3_Win6 Galathea 3 HDMS Vaedderen KT07-14 KT07-14_MC03 KT07-14_MC04 KT07-14_MC07 Latitude of event Longitude of event Maria S. Merian MARUM MC03 MC04 MC07 MSM09/2 MSM09/2_432-5 MSM09/2_453-7 MSM09/2_472-2 MUC MultiCorer Name North Atlantic Ocean Number of e-ribotype Number of genotype Number of morphospecies Number of sequences Paleoenvironmental Reconstructions from Marine Sediments @ AWI Polarstern PS70/239-6 PS70/265-1 PS70/265-2 PS70/299-2 PS70/309-8 PS70 SPACE DAMOCLES PS71/033-12 PS71/085-5 |
description |
Deep-sea sediments constitute a unique archive of ocean change, fueled by a permanent rain of mineral and organic remains from the surface ocean. Until now, paleo-ecological analyses of this archive have been mostly based on information from taxa leaving fossils. In theory, environmental DNA (eDNA) in the sediment has the potential to provide information on non-fossilized taxa, allowing more comprehensive interpretations of the fossil record. Yet, the process controlling the transport and deposition of eDNA onto the sediment and the extent to which it preserves the features of past oceanic biota remains unknown. Planktonic foraminifera are the ideal taxa to allow an assessment of the eDNA signal modification during deposition because their fossils are well preserved in the sediment and their morphological taxonomy is documented by DNA barcodes. Specifically, we re-analyze foraminiferal-specific metabarcodes from 31 deep-sea sediment samples, which were shown to contain a small fraction of sequences from planktonic foraminifera. We confirm that the largest portion of the metabarcode originates from benthic bottom-dwelling foraminifera, representing the in situ community, but a small portion (<10 %) of the metabarcodes can be unambiguously assigned to planktonic taxa. These organisms live exclusively in the surface ocean and the recovered barcodes thus represent an allochthonous component deposited with the rain of organic remains from the surface ocean. We take advantage of the planktonic foraminifera portion of the metabarcodes to establish to what extent the structure of the surface ocean biota is preserved in sedimentary eDNA. We show that planktonic foraminifera DNA is preserved in a range of marine sediment types, the composition of the recovered eDNA metabarcode is replicable and that both the similarity structure and the diversity pattern are preserved. Our results suggest that sedimentary eDNA could preserve the ecological structure of the entire pelagic community, including non-fossilized taxa, thus ... |
format |
Dataset |
author |
Morard, Raphael Lejzerowicz, Franck Darling, Kate F Lecroq-Bennet, Beatrice Pedersen, Mikkel Winther Orlando, Ludovic Pawlowski, Jan Mulitza, Stefan De Vargas, Colomban Kucera, Michal |
author_facet |
Morard, Raphael Lejzerowicz, Franck Darling, Kate F Lecroq-Bennet, Beatrice Pedersen, Mikkel Winther Orlando, Ludovic Pawlowski, Jan Mulitza, Stefan De Vargas, Colomban Kucera, Michal |
author_sort |
Morard, Raphael |
title |
(Supplement 1) Detailed information on environmental samples and sequence data |
title_short |
(Supplement 1) Detailed information on environmental samples and sequence data |
title_full |
(Supplement 1) Detailed information on environmental samples and sequence data |
title_fullStr |
(Supplement 1) Detailed information on environmental samples and sequence data |
title_full_unstemmed |
(Supplement 1) Detailed information on environmental samples and sequence data |
title_sort |
(supplement 1) detailed information on environmental samples and sequence data |
publisher |
PANGAEA |
publishDate |
2016 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.868770 https://doi.org/10.1594/PANGAEA.868770 |
op_coverage |
MEDIAN LATITUDE: 34.312035 * MEDIAN LONGITUDE: 23.499871 * SOUTH-BOUND LATITUDE: -62.010800 * WEST-BOUND LONGITUDE: -65.010000 * NORTH-BOUND LATITUDE: 87.069600 * EAST-BOUND LONGITUDE: 136.310000 * DATE/TIME START: 2007-03-22T00:00:00 * DATE/TIME END: 2008-09-11T19:44:00 * MINIMUM ELEVATION: -5338.0 m * MAXIMUM ELEVATION: -222.0 m |
long_lat |
ENVELOPE(-69.350,-69.350,-69.650,-69.650) ENVELOPE(-65.010000,136.310000,87.069600,-62.010800) |
geographic |
Arctic Arctic Ocean Damocles |
geographic_facet |
Arctic Arctic Ocean Damocles |
genre |
Arctic Arctic Ocean Davis Strait Foraminifera* North Atlantic Planktonic foraminifera |
genre_facet |
Arctic Arctic Ocean Davis Strait Foraminifera* North Atlantic Planktonic foraminifera |
op_source |
Supplement to: Morard, Raphael; Lejzerowicz, Franck; Darling, Kate F; Lecroq-Bennet, Beatrice; Pedersen, Mikkel Winther; Orlando, Ludovic; Pawlowski, Jan; Mulitza, Stefan; De Vargas, Colomban; Kucera, Michal (2017): Planktonic foraminifera-derived environmental DNA extracted from abyssal sediments preserves patterns of plankton macroecology. Biogeosciences, 14, 2741-2754, https://doi.org/10.5194/bg-14-2741-2017 |
op_relation |
Supplement to (3027 KB): Plankton-derived environmental DNA extracted from abyssal sediments preserves patterns of plankton macroecology (URI: https://store.pangaea.de/Publications/Morard-etal_2016/bg-2016-486-supplement.zip) (Supplement 1 - 4) https://doi.pangaea.de/10.1594/PANGAEA.868770 https://doi.org/10.1594/PANGAEA.868770 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.868770 https://doi.org/10.5194/bg-14-2741-2017 |
_version_ |
1766349920832323584 |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.868770 2023-05-15T15:19:43+02:00 (Supplement 1) Detailed information on environmental samples and sequence data Morard, Raphael Lejzerowicz, Franck Darling, Kate F Lecroq-Bennet, Beatrice Pedersen, Mikkel Winther Orlando, Ludovic Pawlowski, Jan Mulitza, Stefan De Vargas, Colomban Kucera, Michal MEDIAN LATITUDE: 34.312035 * MEDIAN LONGITUDE: 23.499871 * SOUTH-BOUND LATITUDE: -62.010800 * WEST-BOUND LONGITUDE: -65.010000 * NORTH-BOUND LATITUDE: 87.069600 * EAST-BOUND LONGITUDE: 136.310000 * DATE/TIME START: 2007-03-22T00:00:00 * DATE/TIME END: 2008-09-11T19:44:00 * MINIMUM ELEVATION: -5338.0 m * MAXIMUM ELEVATION: -222.0 m 2016-11-23 text/tab-separated-values, 496 data points https://doi.pangaea.de/10.1594/PANGAEA.868770 https://doi.org/10.1594/PANGAEA.868770 en eng PANGAEA Supplement to (3027 KB): Plankton-derived environmental DNA extracted from abyssal sediments preserves patterns of plankton macroecology (URI: https://store.pangaea.de/Publications/Morard-etal_2016/bg-2016-486-supplement.zip) (Supplement 1 - 4) https://doi.pangaea.de/10.1594/PANGAEA.868770 https://doi.org/10.1594/PANGAEA.868770 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Morard, Raphael; Lejzerowicz, Franck; Darling, Kate F; Lecroq-Bennet, Beatrice; Pedersen, Mikkel Winther; Orlando, Ludovic; Pawlowski, Jan; Mulitza, Stefan; De Vargas, Colomban; Kucera, Michal (2017): Planktonic foraminifera-derived environmental DNA extracted from abyssal sediments preserves patterns of plankton macroecology. Biogeosciences, 14, 2741-2754, https://doi.org/10.5194/bg-14-2741-2017 ANT-XXIV/2 Arctic Ocean ARK-XXII/2 AWI_Paleo BC Box corer Center for Marine Environmental Sciences Cruise/expedition Date/Time of event Davis Strait Elevation of event Event label Galathea_3_Win3 Galathea_3_Win4 Galathea_3_Win6 Galathea 3 HDMS Vaedderen KT07-14 KT07-14_MC03 KT07-14_MC04 KT07-14_MC07 Latitude of event Longitude of event Maria S. Merian MARUM MC03 MC04 MC07 MSM09/2 MSM09/2_432-5 MSM09/2_453-7 MSM09/2_472-2 MUC MultiCorer Name North Atlantic Ocean Number of e-ribotype Number of genotype Number of morphospecies Number of sequences Paleoenvironmental Reconstructions from Marine Sediments @ AWI Polarstern PS70/239-6 PS70/265-1 PS70/265-2 PS70/299-2 PS70/309-8 PS70 SPACE DAMOCLES PS71/033-12 PS71/085-5 Dataset 2016 ftpangaea https://doi.org/10.1594/PANGAEA.868770 https://doi.org/10.5194/bg-14-2741-2017 2023-01-20T09:08:14Z Deep-sea sediments constitute a unique archive of ocean change, fueled by a permanent rain of mineral and organic remains from the surface ocean. Until now, paleo-ecological analyses of this archive have been mostly based on information from taxa leaving fossils. In theory, environmental DNA (eDNA) in the sediment has the potential to provide information on non-fossilized taxa, allowing more comprehensive interpretations of the fossil record. Yet, the process controlling the transport and deposition of eDNA onto the sediment and the extent to which it preserves the features of past oceanic biota remains unknown. Planktonic foraminifera are the ideal taxa to allow an assessment of the eDNA signal modification during deposition because their fossils are well preserved in the sediment and their morphological taxonomy is documented by DNA barcodes. Specifically, we re-analyze foraminiferal-specific metabarcodes from 31 deep-sea sediment samples, which were shown to contain a small fraction of sequences from planktonic foraminifera. We confirm that the largest portion of the metabarcode originates from benthic bottom-dwelling foraminifera, representing the in situ community, but a small portion (<10 %) of the metabarcodes can be unambiguously assigned to planktonic taxa. These organisms live exclusively in the surface ocean and the recovered barcodes thus represent an allochthonous component deposited with the rain of organic remains from the surface ocean. We take advantage of the planktonic foraminifera portion of the metabarcodes to establish to what extent the structure of the surface ocean biota is preserved in sedimentary eDNA. We show that planktonic foraminifera DNA is preserved in a range of marine sediment types, the composition of the recovered eDNA metabarcode is replicable and that both the similarity structure and the diversity pattern are preserved. Our results suggest that sedimentary eDNA could preserve the ecological structure of the entire pelagic community, including non-fossilized taxa, thus ... Dataset Arctic Arctic Ocean Davis Strait Foraminifera* North Atlantic Planktonic foraminifera PANGAEA - Data Publisher for Earth & Environmental Science Arctic Arctic Ocean Damocles ENVELOPE(-69.350,-69.350,-69.650,-69.650) ENVELOPE(-65.010000,136.310000,87.069600,-62.010800) |