Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis)
Here, we aimed to investigate potential effects of ocean acidification on antimicrobial peptide (AMP) activity in the gills of Mytilus edulis, as gills are directly facing seawater and the changing pH (predicted to be reduced from 8.1 to 7.7 by 2100). The AMP activity of gill and haemocyte extracts...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.868737 2023-05-15T17:37:13+02:00 Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis) Hernroth, Bodil Baden, Susanne Tassidis, H Hörnaeus, K Guillemant, J Bergström Lind, S Bergquist, Jonas 2016-11-23 text/tab-separated-values, 672 data points https://doi.pangaea.de/10.1594/PANGAEA.868737 https://doi.org/10.1594/PANGAEA.868737 en eng PANGAEA Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.868737 https://doi.org/10.1594/PANGAEA.868737 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Hernroth, Bodil; Baden, Susanne; Tassidis, H; Hörnaeus, K; Guillemant, J; Bergström Lind, S; Bergquist, Jonas (2016): Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis). Fish & Shellfish Immunology, 55, 452-459, https://doi.org/10.1016/j.fsi.2016.04.007 Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth inhibition Laboratory experiment Mollusca Mytilus edulis North Atlantic OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH Polar Registration number of species Salinity Single species Species Temperature water Treatment Dataset 2016 ftpangaea https://doi.org/10.1594/PANGAEA.868737 https://doi.org/10.1016/j.fsi.2016.04.007 2023-01-20T09:08:13Z Here, we aimed to investigate potential effects of ocean acidification on antimicrobial peptide (AMP) activity in the gills of Mytilus edulis, as gills are directly facing seawater and the changing pH (predicted to be reduced from 8.1 to 7.7 by 2100). The AMP activity of gill and haemocyte extracts was compared at pH 6.0, 7.7 and 8.1, with a radial diffusion assay against Escherichia coli. The activity of the gill extracts was not affected by pH, while it was significantly reduced with increasing pH in the haemocyte extracts. Gill extracts were also tested against different species of Vibrio (V. parahaemolyticus Vibrio tubiashii, V. splendidus and V. alginoyticus) at pH 7.7 and 8.1. The metabolic activity of the bacteria decreased by 65-90%, depending on species of bacteria, but was, as in the radial diffusion assay, not affected by pH. The results indicated that AMPs from gills are efficient in a broad pH-range. However, when mussels were pre-exposed for pH 7.7 for four month the gill extracts presented significantly lower inhibit of bacterial growth. A full in-depth proteome investigation of gill extracts, using LC-Orbitrap MS/MS technique, showed that among previously described AMPs from haemocytes of Mytilus, myticin A was found up-regulated in response to lipopolysaccharide, 3 h post injection. Sporadic occurrence of other immune related peptides/proteins also pointed to a rapid response (0.5?3 h p.i.). Altogether, our results indicate that the gills of blue mussels constitute an important first line defence adapted to act at the pH of seawater. The antimicrobial activity of the gills is however modulated when mussels are under the pressure of ocean acidification, which may give future advantages for invading pathogens. Dataset North Atlantic Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth inhibition Laboratory experiment Mollusca Mytilus edulis North Atlantic OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH Polar Registration number of species Salinity Single species Species Temperature water Treatment |
spellingShingle |
Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth inhibition Laboratory experiment Mollusca Mytilus edulis North Atlantic OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH Polar Registration number of species Salinity Single species Species Temperature water Treatment Hernroth, Bodil Baden, Susanne Tassidis, H Hörnaeus, K Guillemant, J Bergström Lind, S Bergquist, Jonas Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis) |
topic_facet |
Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth inhibition Laboratory experiment Mollusca Mytilus edulis North Atlantic OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH Polar Registration number of species Salinity Single species Species Temperature water Treatment |
description |
Here, we aimed to investigate potential effects of ocean acidification on antimicrobial peptide (AMP) activity in the gills of Mytilus edulis, as gills are directly facing seawater and the changing pH (predicted to be reduced from 8.1 to 7.7 by 2100). The AMP activity of gill and haemocyte extracts was compared at pH 6.0, 7.7 and 8.1, with a radial diffusion assay against Escherichia coli. The activity of the gill extracts was not affected by pH, while it was significantly reduced with increasing pH in the haemocyte extracts. Gill extracts were also tested against different species of Vibrio (V. parahaemolyticus Vibrio tubiashii, V. splendidus and V. alginoyticus) at pH 7.7 and 8.1. The metabolic activity of the bacteria decreased by 65-90%, depending on species of bacteria, but was, as in the radial diffusion assay, not affected by pH. The results indicated that AMPs from gills are efficient in a broad pH-range. However, when mussels were pre-exposed for pH 7.7 for four month the gill extracts presented significantly lower inhibit of bacterial growth. A full in-depth proteome investigation of gill extracts, using LC-Orbitrap MS/MS technique, showed that among previously described AMPs from haemocytes of Mytilus, myticin A was found up-regulated in response to lipopolysaccharide, 3 h post injection. Sporadic occurrence of other immune related peptides/proteins also pointed to a rapid response (0.5?3 h p.i.). Altogether, our results indicate that the gills of blue mussels constitute an important first line defence adapted to act at the pH of seawater. The antimicrobial activity of the gills is however modulated when mussels are under the pressure of ocean acidification, which may give future advantages for invading pathogens. |
format |
Dataset |
author |
Hernroth, Bodil Baden, Susanne Tassidis, H Hörnaeus, K Guillemant, J Bergström Lind, S Bergquist, Jonas |
author_facet |
Hernroth, Bodil Baden, Susanne Tassidis, H Hörnaeus, K Guillemant, J Bergström Lind, S Bergquist, Jonas |
author_sort |
Hernroth, Bodil |
title |
Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis) |
title_short |
Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis) |
title_full |
Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis) |
title_fullStr |
Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis) |
title_full_unstemmed |
Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis) |
title_sort |
impact of ocean acidification on antimicrobial activity in gills of the blue mussel (mytilus edulis) |
publisher |
PANGAEA |
publishDate |
2016 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.868737 https://doi.org/10.1594/PANGAEA.868737 |
genre |
North Atlantic Ocean acidification |
genre_facet |
North Atlantic Ocean acidification |
op_source |
Supplement to: Hernroth, Bodil; Baden, Susanne; Tassidis, H; Hörnaeus, K; Guillemant, J; Bergström Lind, S; Bergquist, Jonas (2016): Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis). Fish & Shellfish Immunology, 55, 452-459, https://doi.org/10.1016/j.fsi.2016.04.007 |
op_relation |
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.868737 https://doi.org/10.1594/PANGAEA.868737 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.868737 https://doi.org/10.1016/j.fsi.2016.04.007 |
_version_ |
1766137018618740736 |