Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors
The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by differ...
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Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.867318 https://doi.org/10.1594/PANGAEA.867318 |
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.867318 2024-09-15T18:03:13+00:00 Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors Dineshram, R Chandramouli, K Ko, W K Ginger Zhang, Huoming Qian, Pei Yuan Ravasi, Timothy Thiyagarajan, Vengatesen LATITUDE: 36.066670 * LONGITUDE: 120.366670 2016 text/tab-separated-values, 269779 data points https://doi.pangaea.de/10.1594/PANGAEA.867318 https://doi.org/10.1594/PANGAEA.867318 en eng PANGAEA Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.8. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.867318 https://doi.org/10.1594/PANGAEA.867318 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen (2016): Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology, 22(6), 2054-2068, https://doi.org/10.1111/gcb.13249 Accession number Alkalinity total standard deviation Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Crassostrea gigas EXP Experiment Fold change Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Identification Individuals Jiaozhou_Bay Laboratory experiment Mollusca Mortality/Survival North Pacific Number of expressed proteins OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Proteins Registration number of species Salinity Single species Species Survival Temperate dataset 2016 ftpangaea https://doi.org/10.1594/PANGAEA.86731810.1111/gcb.13249 2024-07-24T02:31:33Z The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ-LC-MS/MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down-regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down-regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up-regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs. Dataset Crassostrea gigas Ocean acidification Pacific oyster PANGAEA - Data Publisher for Earth & Environmental Science ENVELOPE(120.366670,120.366670,36.066670,36.066670) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Accession number Alkalinity total standard deviation Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Crassostrea gigas EXP Experiment Fold change Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Identification Individuals Jiaozhou_Bay Laboratory experiment Mollusca Mortality/Survival North Pacific Number of expressed proteins OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Proteins Registration number of species Salinity Single species Species Survival Temperate |
spellingShingle |
Accession number Alkalinity total standard deviation Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Crassostrea gigas EXP Experiment Fold change Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Identification Individuals Jiaozhou_Bay Laboratory experiment Mollusca Mortality/Survival North Pacific Number of expressed proteins OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Proteins Registration number of species Salinity Single species Species Survival Temperate Dineshram, R Chandramouli, K Ko, W K Ginger Zhang, Huoming Qian, Pei Yuan Ravasi, Timothy Thiyagarajan, Vengatesen Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors |
topic_facet |
Accession number Alkalinity total standard deviation Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Crassostrea gigas EXP Experiment Fold change Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Identification Individuals Jiaozhou_Bay Laboratory experiment Mollusca Mortality/Survival North Pacific Number of expressed proteins OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Proteins Registration number of species Salinity Single species Species Survival Temperate |
description |
The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ-LC-MS/MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down-regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down-regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up-regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs. |
format |
Dataset |
author |
Dineshram, R Chandramouli, K Ko, W K Ginger Zhang, Huoming Qian, Pei Yuan Ravasi, Timothy Thiyagarajan, Vengatesen |
author_facet |
Dineshram, R Chandramouli, K Ko, W K Ginger Zhang, Huoming Qian, Pei Yuan Ravasi, Timothy Thiyagarajan, Vengatesen |
author_sort |
Dineshram, R |
title |
Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors |
title_short |
Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors |
title_full |
Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors |
title_fullStr |
Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors |
title_full_unstemmed |
Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors |
title_sort |
quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors |
publisher |
PANGAEA |
publishDate |
2016 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.867318 https://doi.org/10.1594/PANGAEA.867318 |
op_coverage |
LATITUDE: 36.066670 * LONGITUDE: 120.366670 |
long_lat |
ENVELOPE(120.366670,120.366670,36.066670,36.066670) |
genre |
Crassostrea gigas Ocean acidification Pacific oyster |
genre_facet |
Crassostrea gigas Ocean acidification Pacific oyster |
op_source |
Supplement to: Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen (2016): Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology, 22(6), 2054-2068, https://doi.org/10.1111/gcb.13249 |
op_relation |
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.8. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.867318 https://doi.org/10.1594/PANGAEA.867318 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.86731810.1111/gcb.13249 |
_version_ |
1810440728696848384 |