Viral, bacterial and ciliate abundance and viral-bacterial diversity in mesocosm experiments, 2007-2008, Kongsfjorden
Two mesocosm experiments, PAME-I and PAME-II were conducted in 2007 and 2008 to investigate fate of organic carbon in the arctic microbial food web. Mesocosms were nutrient fertilized initially to induce phytoplankton bloom development. In PAME-I eight units (each 700 L) formed two four point gradie...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.865353 2024-05-19T07:36:09+00:00 Viral, bacterial and ciliate abundance and viral-bacterial diversity in mesocosm experiments, 2007-2008, Kongsfjorden Sandaa, Ruth-Anne Larsen, Aud Thyrhaug, Runar Egge, Jorun K Töpper, Birte LATITUDE: 78.953500 * LONGITUDE: 11.956000 * DATE/TIME START: 2007-08-02T00:00:00 * DATE/TIME END: 2008-07-10T00:00:00 * MINIMUM ELEVATION: -25.0 m * MAXIMUM ELEVATION: 0.0 m 2016 text/tab-separated-values, 50 data points https://doi.pangaea.de/10.1594/PANGAEA.865353 https://doi.org/10.1594/PANGAEA.865353 en eng PANGAEA All files of Sandaa et al. 2016 in one zip archive (URI: https://store.pangaea.de/Publications/Sandaa-etal_2016/Sandaa-etal_files.zip) https://doi.pangaea.de/10.1594/PANGAEA.865353 https://doi.org/10.1594/PANGAEA.865353 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Sandaa, Ruth-Anne; Pree, Bernadette; Larsen, Aud; Våge, Selina; Töpper, Birte; Töpper, Joachim P; Thyrhaug, Runar; Thingstad, Tron Frede (2017): The Response of heterotrophic prokaryote and viral communities to labile organic carbon inputs is controlled by the predator food chain structure. Viruses, 9(9), 238, https://doi.org/10.3390/v9090238 DATE/TIME File content File format File size Kongsfjorden-mesocosm MESO Mesocosm experiment Principal investigator Svalbard Uniform resource locator/link to file Dataset 2016 ftpangaea https://doi.org/10.1594/PANGAEA.86535310.3390/v9090238 2024-04-30T23:34:34Z Two mesocosm experiments, PAME-I and PAME-II were conducted in 2007 and 2008 to investigate fate of organic carbon in the arctic microbial food web. Mesocosms were nutrient fertilized initially to induce phytoplankton bloom development. In PAME-I eight units (each 700 L) formed two four point gradients of additional DOC in form of glucose (0, 0.5, 1 and 3 times Redfield ratio in terms of carbon relative to the nitrogen and phosphorus additions) (Fig. 1). All the eight units also got a daily dose of NH4+ and PO4**3- in Redfield ratio. Two gradients were set up, one with silicate addition, performed in the Arctic location Ny Ålesund, Svalbard, have previously been reported to give different food-web level responses to similar nutrient perturbations. In PAME-II all ten units (each 900 L) formed two four point gradients of additional DOC in form of glucose (0, 0.5, 1, 2 and 3 times Redfield ratio in terms of carbon relative to nitrogen and phosphorus additions). The two gradients in glucose were kept silicate replete. NH4+ was used as the DIN source in one gradient (units 1 to 5) and NO3- in the other (units 6-9). All units got a daily dose of PO4**3- in Redfield ratio. Prokaryotes and viruses were measured by flow cytometry, while ciliate abundances were counted using a Flow Cam. Viral and bacterial diversity was measured by PFGE and DGGE, respectively. In PAME-II the abundance of ciliates was lower than in PAME-I, presumably caused by higher copepod grazing. The abundances of prokaryotes and viruses were also lower in PAME-II compared to PAME-I. Further, less diversity was detected in the viral community (FCM and PFGE) in PAME-II, and no response was observed in the bacterial community structure due to addition of organic carbon. Dataset Arctic Kongsfjord* Kongsfjorden Ny Ålesund Ny-Ålesund PAME Phytoplankton Svalbard PANGAEA - Data Publisher for Earth & Environmental Science ENVELOPE(11.956000,11.956000,78.953500,78.953500) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
DATE/TIME File content File format File size Kongsfjorden-mesocosm MESO Mesocosm experiment Principal investigator Svalbard Uniform resource locator/link to file |
spellingShingle |
DATE/TIME File content File format File size Kongsfjorden-mesocosm MESO Mesocosm experiment Principal investigator Svalbard Uniform resource locator/link to file Sandaa, Ruth-Anne Larsen, Aud Thyrhaug, Runar Egge, Jorun K Töpper, Birte Viral, bacterial and ciliate abundance and viral-bacterial diversity in mesocosm experiments, 2007-2008, Kongsfjorden |
topic_facet |
DATE/TIME File content File format File size Kongsfjorden-mesocosm MESO Mesocosm experiment Principal investigator Svalbard Uniform resource locator/link to file |
description |
Two mesocosm experiments, PAME-I and PAME-II were conducted in 2007 and 2008 to investigate fate of organic carbon in the arctic microbial food web. Mesocosms were nutrient fertilized initially to induce phytoplankton bloom development. In PAME-I eight units (each 700 L) formed two four point gradients of additional DOC in form of glucose (0, 0.5, 1 and 3 times Redfield ratio in terms of carbon relative to the nitrogen and phosphorus additions) (Fig. 1). All the eight units also got a daily dose of NH4+ and PO4**3- in Redfield ratio. Two gradients were set up, one with silicate addition, performed in the Arctic location Ny Ålesund, Svalbard, have previously been reported to give different food-web level responses to similar nutrient perturbations. In PAME-II all ten units (each 900 L) formed two four point gradients of additional DOC in form of glucose (0, 0.5, 1, 2 and 3 times Redfield ratio in terms of carbon relative to nitrogen and phosphorus additions). The two gradients in glucose were kept silicate replete. NH4+ was used as the DIN source in one gradient (units 1 to 5) and NO3- in the other (units 6-9). All units got a daily dose of PO4**3- in Redfield ratio. Prokaryotes and viruses were measured by flow cytometry, while ciliate abundances were counted using a Flow Cam. Viral and bacterial diversity was measured by PFGE and DGGE, respectively. In PAME-II the abundance of ciliates was lower than in PAME-I, presumably caused by higher copepod grazing. The abundances of prokaryotes and viruses were also lower in PAME-II compared to PAME-I. Further, less diversity was detected in the viral community (FCM and PFGE) in PAME-II, and no response was observed in the bacterial community structure due to addition of organic carbon. |
format |
Dataset |
author |
Sandaa, Ruth-Anne Larsen, Aud Thyrhaug, Runar Egge, Jorun K Töpper, Birte |
author_facet |
Sandaa, Ruth-Anne Larsen, Aud Thyrhaug, Runar Egge, Jorun K Töpper, Birte |
author_sort |
Sandaa, Ruth-Anne |
title |
Viral, bacterial and ciliate abundance and viral-bacterial diversity in mesocosm experiments, 2007-2008, Kongsfjorden |
title_short |
Viral, bacterial and ciliate abundance and viral-bacterial diversity in mesocosm experiments, 2007-2008, Kongsfjorden |
title_full |
Viral, bacterial and ciliate abundance and viral-bacterial diversity in mesocosm experiments, 2007-2008, Kongsfjorden |
title_fullStr |
Viral, bacterial and ciliate abundance and viral-bacterial diversity in mesocosm experiments, 2007-2008, Kongsfjorden |
title_full_unstemmed |
Viral, bacterial and ciliate abundance and viral-bacterial diversity in mesocosm experiments, 2007-2008, Kongsfjorden |
title_sort |
viral, bacterial and ciliate abundance and viral-bacterial diversity in mesocosm experiments, 2007-2008, kongsfjorden |
publisher |
PANGAEA |
publishDate |
2016 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.865353 https://doi.org/10.1594/PANGAEA.865353 |
op_coverage |
LATITUDE: 78.953500 * LONGITUDE: 11.956000 * DATE/TIME START: 2007-08-02T00:00:00 * DATE/TIME END: 2008-07-10T00:00:00 * MINIMUM ELEVATION: -25.0 m * MAXIMUM ELEVATION: 0.0 m |
long_lat |
ENVELOPE(11.956000,11.956000,78.953500,78.953500) |
genre |
Arctic Kongsfjord* Kongsfjorden Ny Ålesund Ny-Ålesund PAME Phytoplankton Svalbard |
genre_facet |
Arctic Kongsfjord* Kongsfjorden Ny Ålesund Ny-Ålesund PAME Phytoplankton Svalbard |
op_source |
Supplement to: Sandaa, Ruth-Anne; Pree, Bernadette; Larsen, Aud; Våge, Selina; Töpper, Birte; Töpper, Joachim P; Thyrhaug, Runar; Thingstad, Tron Frede (2017): The Response of heterotrophic prokaryote and viral communities to labile organic carbon inputs is controlled by the predator food chain structure. Viruses, 9(9), 238, https://doi.org/10.3390/v9090238 |
op_relation |
All files of Sandaa et al. 2016 in one zip archive (URI: https://store.pangaea.de/Publications/Sandaa-etal_2016/Sandaa-etal_files.zip) https://doi.pangaea.de/10.1594/PANGAEA.865353 https://doi.org/10.1594/PANGAEA.865353 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.86535310.3390/v9090238 |
_version_ |
1799475201915748352 |