Differential impacts of elevated CO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod
Ocean acidification impacts fish and other marine species through increased seawater PCO2 levels (hypercapnia). Knowledge of the physiological mechanisms mediating effects in various tissues of fish is incomplete. Here we tested the effects of extracellular hypercapnia and acidosis on energy metabol...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.861259 2023-05-15T15:27:23+02:00 Differential impacts of elevated CO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod Stapp, Laura Kreiss, Cornelia M Pörtner, Hans-Otto Lannig, Gisela LATITUDE: 54.180000 * LONGITUDE: 7.900000 2016-06-07 application/zip, 70.1 kBytes https://doi.pangaea.de/10.1594/PANGAEA.861259 https://doi.org/10.1594/PANGAEA.861259 en eng PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.861259 https://doi.org/10.1594/PANGAEA.861259 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Stapp, Laura; Kreiss, Cornelia M; Pörtner, Hans-Otto; Lannig, Gisela (2015): Differential impacts of elevated CO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod, Gadus morhua. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 187, 160-167, https://doi.org/10.1016/j.cbpa.2015.05.009 BIOACID Biological Impacts of Ocean Acidification Helgoland North Sea MULT Multiple investigations Dataset 2016 ftpangaea https://doi.org/10.1594/PANGAEA.861259 https://doi.org/10.1016/j.cbpa.2015.05.009 2023-01-20T09:07:21Z Ocean acidification impacts fish and other marine species through increased seawater PCO2 levels (hypercapnia). Knowledge of the physiological mechanisms mediating effects in various tissues of fish is incomplete. Here we tested the effects of extracellular hypercapnia and acidosis on energy metabolism of gill and liver cells of Atlantic cod. Exposure media mimicked blood conditions in vivo, either during normo- or hypercapnia and at control or acidic extracellular pH (pHe). We determined metabolic rate and energy expenditure for protein biosynthesis, Na+/K+-ATPase and H+-ATPase and considered nutrition status by measurements of metabolic rate and protein biosynthesis in media with and without free amino acids (FAA). Addition of FAA stimulated hepatic but not branchial oxygen consumption. Normo- and hypercapnic acidosis as well as hypercapnia at control pHe depressed metabolic stimulation of hepatocytes. In gill cells, acidosis depressed respiration independent of PCO2 and FAA levels. For both cell types, depressed respiration was not correlated with the same reduction in energy allocated to protein biosynthesis or Na+/K+-ATPase. Hepatic energy expenditure for protein synthesis and Na+/K+- ATPase was even elevated at acidic compared to control pHe suggesting increased costs for ion regulation and cel- lular reorganization. Hypercapnia at control pHe strongly reduced oxygen demand of branchial Na+/K+-ATPase with a similar trend for H+-ATPase. We conclude that extracellular acidosis triggers metabolic depression in gill and metabolically stimulated liver cells. Additionally, hypercapnia itself seems to limit capacities for metabolic usage of amino acids in liver cells while it decreases the use and costs of ion regulatory ATPases in gill cells. Dataset atlantic cod Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science Helgoland ENVELOPE(7.900000,7.900000,54.180000,54.180000) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
BIOACID Biological Impacts of Ocean Acidification Helgoland North Sea MULT Multiple investigations |
spellingShingle |
BIOACID Biological Impacts of Ocean Acidification Helgoland North Sea MULT Multiple investigations Stapp, Laura Kreiss, Cornelia M Pörtner, Hans-Otto Lannig, Gisela Differential impacts of elevated CO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod |
topic_facet |
BIOACID Biological Impacts of Ocean Acidification Helgoland North Sea MULT Multiple investigations |
description |
Ocean acidification impacts fish and other marine species through increased seawater PCO2 levels (hypercapnia). Knowledge of the physiological mechanisms mediating effects in various tissues of fish is incomplete. Here we tested the effects of extracellular hypercapnia and acidosis on energy metabolism of gill and liver cells of Atlantic cod. Exposure media mimicked blood conditions in vivo, either during normo- or hypercapnia and at control or acidic extracellular pH (pHe). We determined metabolic rate and energy expenditure for protein biosynthesis, Na+/K+-ATPase and H+-ATPase and considered nutrition status by measurements of metabolic rate and protein biosynthesis in media with and without free amino acids (FAA). Addition of FAA stimulated hepatic but not branchial oxygen consumption. Normo- and hypercapnic acidosis as well as hypercapnia at control pHe depressed metabolic stimulation of hepatocytes. In gill cells, acidosis depressed respiration independent of PCO2 and FAA levels. For both cell types, depressed respiration was not correlated with the same reduction in energy allocated to protein biosynthesis or Na+/K+-ATPase. Hepatic energy expenditure for protein synthesis and Na+/K+- ATPase was even elevated at acidic compared to control pHe suggesting increased costs for ion regulation and cel- lular reorganization. Hypercapnia at control pHe strongly reduced oxygen demand of branchial Na+/K+-ATPase with a similar trend for H+-ATPase. We conclude that extracellular acidosis triggers metabolic depression in gill and metabolically stimulated liver cells. Additionally, hypercapnia itself seems to limit capacities for metabolic usage of amino acids in liver cells while it decreases the use and costs of ion regulatory ATPases in gill cells. |
format |
Dataset |
author |
Stapp, Laura Kreiss, Cornelia M Pörtner, Hans-Otto Lannig, Gisela |
author_facet |
Stapp, Laura Kreiss, Cornelia M Pörtner, Hans-Otto Lannig, Gisela |
author_sort |
Stapp, Laura |
title |
Differential impacts of elevated CO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod |
title_short |
Differential impacts of elevated CO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod |
title_full |
Differential impacts of elevated CO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod |
title_fullStr |
Differential impacts of elevated CO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod |
title_full_unstemmed |
Differential impacts of elevated CO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod |
title_sort |
differential impacts of elevated co2 and acidosis on the energy budget of gill and liver cells from atlantic cod |
publisher |
PANGAEA |
publishDate |
2016 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.861259 https://doi.org/10.1594/PANGAEA.861259 |
op_coverage |
LATITUDE: 54.180000 * LONGITUDE: 7.900000 |
long_lat |
ENVELOPE(7.900000,7.900000,54.180000,54.180000) |
geographic |
Helgoland |
geographic_facet |
Helgoland |
genre |
atlantic cod Ocean acidification |
genre_facet |
atlantic cod Ocean acidification |
op_source |
Supplement to: Stapp, Laura; Kreiss, Cornelia M; Pörtner, Hans-Otto; Lannig, Gisela (2015): Differential impacts of elevated CO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod, Gadus morhua. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 187, 160-167, https://doi.org/10.1016/j.cbpa.2015.05.009 |
op_relation |
https://doi.pangaea.de/10.1594/PANGAEA.861259 https://doi.org/10.1594/PANGAEA.861259 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.861259 https://doi.org/10.1016/j.cbpa.2015.05.009 |
_version_ |
1766357823313149952 |