Interactive effects of salinity and elevated CO2 levels on juvenile eastern oysters, Crassostrea virginica
Rising levels of atmospheric CO2 lead to acidification of the ocean and alter seawater carbonate chemistry, which can negatively impact calcifying organisms, including mollusks. In estuaries, exposure to elevated CO2 levels often co-occurs with other stressors, such as reduced salinity, which enhanc...
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Format: | Dataset |
Language: | English |
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PANGAEA
2012
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Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.860868 https://doi.org/10.1594/PANGAEA.860868 |
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.860868 |
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openpolar |
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Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Acetate Adenosine diphosphate per wet mass Adenosine diphosphate/adenosine triphosphate ratio Adenosine monophosphate Adenosine triphosphate Adenosine triphosphate+adenosine diphosphate+adenosine monophosphate Adenylate energy charge Alanine Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Betaine Bicarbonate ion Biomass/Abundance/Elemental composition Brackish waters Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Carbonic anhydrase activity per protein Comment |
spellingShingle |
Acetate Adenosine diphosphate per wet mass Adenosine diphosphate/adenosine triphosphate ratio Adenosine monophosphate Adenosine triphosphate Adenosine triphosphate+adenosine diphosphate+adenosine monophosphate Adenylate energy charge Alanine Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Betaine Bicarbonate ion Biomass/Abundance/Elemental composition Brackish waters Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Carbonic anhydrase activity per protein Comment Dickinson, Gary H Ivanina, Anna Matoo, Omera B Pörtner, Hans-Otto Lannig, Gisela Bock, C Beniash, Elia Sokolova, Inna M Interactive effects of salinity and elevated CO2 levels on juvenile eastern oysters, Crassostrea virginica |
topic_facet |
Acetate Adenosine diphosphate per wet mass Adenosine diphosphate/adenosine triphosphate ratio Adenosine monophosphate Adenosine triphosphate Adenosine triphosphate+adenosine diphosphate+adenosine monophosphate Adenylate energy charge Alanine Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Betaine Bicarbonate ion Biomass/Abundance/Elemental composition Brackish waters Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Carbonic anhydrase activity per protein Comment |
description |
Rising levels of atmospheric CO2 lead to acidification of the ocean and alter seawater carbonate chemistry, which can negatively impact calcifying organisms, including mollusks. In estuaries, exposure to elevated CO2 levels often co-occurs with other stressors, such as reduced salinity, which enhances the acidification trend, affects ion and acid-base regulation of estuarine calcifiers and modifies their response to ocean acidification. We studied the interactive effects of salinity and partial pressure of CO2 (PCO2) on biomineralization and energy homeostasis in juveniles of the eastern oyster, Crassostrea virginica, a common estuarine bivalve. Juveniles were exposed for 11 weeks to one of two environmentally relevant salinities (30 or 15 PSU) either at current atmospheric PCO2 (400 µatm, normocapnia) or PCO2 projected by moderate IPCC scenarios for the year 2100 (700-800 µatm, hypercapnia). Exposure of the juvenile oysters to elevated PCO2 and/or low salinity led to a significant increase in mortality, reduction of tissue energy stores (glycogen and lipid) and negative soft tissue growth, indicating energy deficiency. Interestingly, tissue ATP levels were not affected by exposure to changing salinity and PCO2, suggesting that juvenile oysters maintain their cellular energy status at the expense of lipid and glycogen stores. At the same time, no compensatory upregulation of carbonic anhydrase activity was found under the conditions of low salinity and high PCO2. Metabolic profiling using magnetic resonance spectroscopy revealed altered metabolite status following low salinity exposure; specifically, acetate levels were lower in hypercapnic than in normocapnic individuals at low salinity. Combined exposure to hypercapnia and low salinity negatively affected mechanical properties of shells of the juveniles, resulting in reduced hardness and fracture resistance. Thus, our data suggest that the combined effects of elevated PCO2 and fluctuating salinity may jeopardize the survival of eastern oysters because of ... |
format |
Dataset |
author |
Dickinson, Gary H Ivanina, Anna Matoo, Omera B Pörtner, Hans-Otto Lannig, Gisela Bock, C Beniash, Elia Sokolova, Inna M |
author_facet |
Dickinson, Gary H Ivanina, Anna Matoo, Omera B Pörtner, Hans-Otto Lannig, Gisela Bock, C Beniash, Elia Sokolova, Inna M |
author_sort |
Dickinson, Gary H |
title |
Interactive effects of salinity and elevated CO2 levels on juvenile eastern oysters, Crassostrea virginica |
title_short |
Interactive effects of salinity and elevated CO2 levels on juvenile eastern oysters, Crassostrea virginica |
title_full |
Interactive effects of salinity and elevated CO2 levels on juvenile eastern oysters, Crassostrea virginica |
title_fullStr |
Interactive effects of salinity and elevated CO2 levels on juvenile eastern oysters, Crassostrea virginica |
title_full_unstemmed |
Interactive effects of salinity and elevated CO2 levels on juvenile eastern oysters, Crassostrea virginica |
title_sort |
interactive effects of salinity and elevated co2 levels on juvenile eastern oysters, crassostrea virginica |
publisher |
PANGAEA |
publishDate |
2012 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.860868 https://doi.org/10.1594/PANGAEA.860868 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Supplement to: Dickinson, Gary H; Ivanina, Anna; Matoo, Omera B; Pörtner, Hans-Otto; Lannig, Gisela; Bock, C; Beniash, Elia; Sokolova, Inna M (2011): Interactive effects of salinity and elevated CO2 levels on juvenile eastern oysters, Crassostrea virginica. Journal of Experimental Biology, 215(1), 29-43, https://doi.org/10.1242/jeb.061481 |
op_relation |
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.8. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.860868 https://doi.org/10.1594/PANGAEA.860868 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.860868 https://doi.org/10.1242/jeb.061481 |
_version_ |
1766159555780149248 |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.860868 2023-05-15T17:52:11+02:00 Interactive effects of salinity and elevated CO2 levels on juvenile eastern oysters, Crassostrea virginica Dickinson, Gary H Ivanina, Anna Matoo, Omera B Pörtner, Hans-Otto Lannig, Gisela Bock, C Beniash, Elia Sokolova, Inna M 2012-05-30 text/tab-separated-values, 9613 data points https://doi.pangaea.de/10.1594/PANGAEA.860868 https://doi.org/10.1594/PANGAEA.860868 en eng PANGAEA Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.8. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.860868 https://doi.org/10.1594/PANGAEA.860868 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Dickinson, Gary H; Ivanina, Anna; Matoo, Omera B; Pörtner, Hans-Otto; Lannig, Gisela; Bock, C; Beniash, Elia; Sokolova, Inna M (2011): Interactive effects of salinity and elevated CO2 levels on juvenile eastern oysters, Crassostrea virginica. Journal of Experimental Biology, 215(1), 29-43, https://doi.org/10.1242/jeb.061481 Acetate Adenosine diphosphate per wet mass Adenosine diphosphate/adenosine triphosphate ratio Adenosine monophosphate Adenosine triphosphate Adenosine triphosphate+adenosine diphosphate+adenosine monophosphate Adenylate energy charge Alanine Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Betaine Bicarbonate ion Biomass/Abundance/Elemental composition Brackish waters Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Carbonic anhydrase activity per protein Comment Dataset 2012 ftpangaea https://doi.org/10.1594/PANGAEA.860868 https://doi.org/10.1242/jeb.061481 2023-01-20T09:07:19Z Rising levels of atmospheric CO2 lead to acidification of the ocean and alter seawater carbonate chemistry, which can negatively impact calcifying organisms, including mollusks. In estuaries, exposure to elevated CO2 levels often co-occurs with other stressors, such as reduced salinity, which enhances the acidification trend, affects ion and acid-base regulation of estuarine calcifiers and modifies their response to ocean acidification. We studied the interactive effects of salinity and partial pressure of CO2 (PCO2) on biomineralization and energy homeostasis in juveniles of the eastern oyster, Crassostrea virginica, a common estuarine bivalve. Juveniles were exposed for 11 weeks to one of two environmentally relevant salinities (30 or 15 PSU) either at current atmospheric PCO2 (400 µatm, normocapnia) or PCO2 projected by moderate IPCC scenarios for the year 2100 (700-800 µatm, hypercapnia). Exposure of the juvenile oysters to elevated PCO2 and/or low salinity led to a significant increase in mortality, reduction of tissue energy stores (glycogen and lipid) and negative soft tissue growth, indicating energy deficiency. Interestingly, tissue ATP levels were not affected by exposure to changing salinity and PCO2, suggesting that juvenile oysters maintain their cellular energy status at the expense of lipid and glycogen stores. At the same time, no compensatory upregulation of carbonic anhydrase activity was found under the conditions of low salinity and high PCO2. Metabolic profiling using magnetic resonance spectroscopy revealed altered metabolite status following low salinity exposure; specifically, acetate levels were lower in hypercapnic than in normocapnic individuals at low salinity. Combined exposure to hypercapnia and low salinity negatively affected mechanical properties of shells of the juveniles, resulting in reduced hardness and fracture resistance. Thus, our data suggest that the combined effects of elevated PCO2 and fluctuating salinity may jeopardize the survival of eastern oysters because of ... Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |