Composition of bacterioplankton in the North Atlantic subtropical gyre
Subtropical oceanic gyres are the most extensive biomes on Earth where SAR11 and Prochlorococcus bacterioplankton numerically dominate the surface waters depleted in inorganic macronutrients as well as in dissolved organic matter. In such nutrient poor conditions bacterioplankton could become photoh...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.860819 2024-09-15T18:22:55+00:00 Composition of bacterioplankton in the North Atlantic subtropical gyre Gomez-Pereira, Paola R Hartmann, Manuela Grob, C Tarran, Glen A Martin, Adrian Peter Fuchs, Bernhard M Scanlan, David J Zubkov, Mikhail V MEDIAN LATITUDE: 21.374000 * MEDIAN LONGITUDE: 33.906000 * SOUTH-BOUND LATITUDE: 4.800000 * WEST-BOUND LONGITUDE: 28.160000 * NORTH-BOUND LATITUDE: 34.210000 * EAST-BOUND LONGITUDE: 41.110000 * MINIMUM DEPTH, water: 20 m * MAXIMUM DEPTH, water: 20 m text/tab-separated-values, 36 data points https://doi.pangaea.de/10.1594/PANGAEA.860819 en eng PANGAEA Oligonucleotide probes and formamide concentration used in Gomez-Pereira et al., 2013 (URI: https://store.pangaea.de/Publications/Gomez-Pereira-etal_2013/Oligonucleotide-probes_Gomez-Pereira-etal_2013.pdf) https://doi.pangaea.de/10.1594/PANGAEA.860819 Access constraints: access rights needed info:eu-repo/semantics/restrictedAccess Supplement to: Gomez-Pereira, Paola R; Hartmann, Manuela; Grob, C; Tarran, Glen A; Martin, Adrian Peter; Fuchs, Bernhard M; Scanlan, David J; Zubkov, Mikhail V (2012): Comparable light stimulation of organic nutrient uptake by SAR11 and Prochlorococcus in the North Atlantic subtropical gyre. The ISME Journal, 7(3), 603-614, https://doi.org/10.1038/ismej.2012.126 Bacteria targed with EUB338(I-III) oligonucleotide FISH-probe Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) DEPTH water LATITUDE LONGITUDE Prochlorococcus targeted with PRO405 oligonucleotide FISH-probe SAR11 dataset ftpangaea https://doi.org/10.1038/ismej.2012.126 2024-07-24T02:31:33Z Subtropical oceanic gyres are the most extensive biomes on Earth where SAR11 and Prochlorococcus bacterioplankton numerically dominate the surface waters depleted in inorganic macronutrients as well as in dissolved organic matter. In such nutrient poor conditions bacterioplankton could become photoheterotrophic. We assessed the photoheterotrophy of the key microbial taxa in the North Atlantic oligotrophic gyre and adjacent regions. The experimental work was performed on board the Royal Research Ship James Cook (cruise no. JC53, October-November 2010) as part of the Atlantic Meridional Transect programme, and on board the Royal Research Ship Discovery (cruise no. D369, August-September 2011). At each station, samples were collected from 20m depth with a sampling rosette of 20-l Niskin bottles mounted on aconductivity-temperature-depth profiler. Samples were collected in 1 l thermos flasks (washed with10% v/v HCl) in the dark and processed immediately. Depth of 20m was chosen because it represents the mixed layer and it was the shallowest depth unaffected by the ship's movement, including thrusting, that could artificially affect microbial metabolism in nutrient-depleted stratified surfacewaters. Molecular identification of flow-sorted cells CARD-FISH was performed on flow-sorted cells to identify the groups for which uptake rates were measured. High nucleic acid-containing bacteria, based on SYBR Green DNA staining, that had virtually undetectable chlorophyll autofluorescence, were phylogenetically affiliated with Prochlorococcus,in agreement with our previously reported results (Zubkov et al., 2007; doi:10.1111/j.1462-2920.2007.01324.x). Dataset North Atlantic PANGAEA - Data Publisher for Earth & Environmental Science ENVELOPE(28.160000,41.110000,34.210000,4.800000) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Bacteria targed with EUB338(I-III) oligonucleotide FISH-probe Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) DEPTH water LATITUDE LONGITUDE Prochlorococcus targeted with PRO405 oligonucleotide FISH-probe SAR11 |
spellingShingle |
Bacteria targed with EUB338(I-III) oligonucleotide FISH-probe Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) DEPTH water LATITUDE LONGITUDE Prochlorococcus targeted with PRO405 oligonucleotide FISH-probe SAR11 Gomez-Pereira, Paola R Hartmann, Manuela Grob, C Tarran, Glen A Martin, Adrian Peter Fuchs, Bernhard M Scanlan, David J Zubkov, Mikhail V Composition of bacterioplankton in the North Atlantic subtropical gyre |
topic_facet |
Bacteria targed with EUB338(I-III) oligonucleotide FISH-probe Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) DEPTH water LATITUDE LONGITUDE Prochlorococcus targeted with PRO405 oligonucleotide FISH-probe SAR11 |
description |
Subtropical oceanic gyres are the most extensive biomes on Earth where SAR11 and Prochlorococcus bacterioplankton numerically dominate the surface waters depleted in inorganic macronutrients as well as in dissolved organic matter. In such nutrient poor conditions bacterioplankton could become photoheterotrophic. We assessed the photoheterotrophy of the key microbial taxa in the North Atlantic oligotrophic gyre and adjacent regions. The experimental work was performed on board the Royal Research Ship James Cook (cruise no. JC53, October-November 2010) as part of the Atlantic Meridional Transect programme, and on board the Royal Research Ship Discovery (cruise no. D369, August-September 2011). At each station, samples were collected from 20m depth with a sampling rosette of 20-l Niskin bottles mounted on aconductivity-temperature-depth profiler. Samples were collected in 1 l thermos flasks (washed with10% v/v HCl) in the dark and processed immediately. Depth of 20m was chosen because it represents the mixed layer and it was the shallowest depth unaffected by the ship's movement, including thrusting, that could artificially affect microbial metabolism in nutrient-depleted stratified surfacewaters. Molecular identification of flow-sorted cells CARD-FISH was performed on flow-sorted cells to identify the groups for which uptake rates were measured. High nucleic acid-containing bacteria, based on SYBR Green DNA staining, that had virtually undetectable chlorophyll autofluorescence, were phylogenetically affiliated with Prochlorococcus,in agreement with our previously reported results (Zubkov et al., 2007; doi:10.1111/j.1462-2920.2007.01324.x). |
format |
Dataset |
author |
Gomez-Pereira, Paola R Hartmann, Manuela Grob, C Tarran, Glen A Martin, Adrian Peter Fuchs, Bernhard M Scanlan, David J Zubkov, Mikhail V |
author_facet |
Gomez-Pereira, Paola R Hartmann, Manuela Grob, C Tarran, Glen A Martin, Adrian Peter Fuchs, Bernhard M Scanlan, David J Zubkov, Mikhail V |
author_sort |
Gomez-Pereira, Paola R |
title |
Composition of bacterioplankton in the North Atlantic subtropical gyre |
title_short |
Composition of bacterioplankton in the North Atlantic subtropical gyre |
title_full |
Composition of bacterioplankton in the North Atlantic subtropical gyre |
title_fullStr |
Composition of bacterioplankton in the North Atlantic subtropical gyre |
title_full_unstemmed |
Composition of bacterioplankton in the North Atlantic subtropical gyre |
title_sort |
composition of bacterioplankton in the north atlantic subtropical gyre |
publisher |
PANGAEA |
url |
https://doi.pangaea.de/10.1594/PANGAEA.860819 |
op_coverage |
MEDIAN LATITUDE: 21.374000 * MEDIAN LONGITUDE: 33.906000 * SOUTH-BOUND LATITUDE: 4.800000 * WEST-BOUND LONGITUDE: 28.160000 * NORTH-BOUND LATITUDE: 34.210000 * EAST-BOUND LONGITUDE: 41.110000 * MINIMUM DEPTH, water: 20 m * MAXIMUM DEPTH, water: 20 m |
long_lat |
ENVELOPE(28.160000,41.110000,34.210000,4.800000) |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Supplement to: Gomez-Pereira, Paola R; Hartmann, Manuela; Grob, C; Tarran, Glen A; Martin, Adrian Peter; Fuchs, Bernhard M; Scanlan, David J; Zubkov, Mikhail V (2012): Comparable light stimulation of organic nutrient uptake by SAR11 and Prochlorococcus in the North Atlantic subtropical gyre. The ISME Journal, 7(3), 603-614, https://doi.org/10.1038/ismej.2012.126 |
op_relation |
Oligonucleotide probes and formamide concentration used in Gomez-Pereira et al., 2013 (URI: https://store.pangaea.de/Publications/Gomez-Pereira-etal_2013/Oligonucleotide-probes_Gomez-Pereira-etal_2013.pdf) https://doi.pangaea.de/10.1594/PANGAEA.860819 |
op_rights |
Access constraints: access rights needed info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1038/ismej.2012.126 |
_version_ |
1810462956956155904 |