Relative abundance of anaerobic methanotrophic archaea in bacterial mat Cascadia margin off Oregon

• Main Authors: Knittel, Katrin, Lösekann, Tina, Boetius, Antje, Kort, Renate, Amann, Rudolf
• Format: Dataset
• Language: English
• Published: PANGAEA 2016
• Subjects: Anaerobic methanotrophic archaea-1; targeted with ANME-1-350 oligonucleotide FISH-probe; Cascadia Margin; Date/Time of event; DEPTH; water; Event label; Fluorescence in situ hybridization (FISH); Habitat; Latitude of event; Longitude of event; Multicorer with television; SO143_105-1; SO143_139; SO143_185-1; SO143/2; SO148/1; SO148/1_19-2; SO148/1_38; SO148/1_51; Sonne; Standard deviation; TECFLUX I; TECFLUX II; TVMUC; TV-MUC-10; TV-MUC-12; TV-MUC-6; Methane hydrate
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.860405; https://doi.org/10.1594/PANGAEA.860405

The microbially mediated anaerobic oxidation of methane (AOM) is the major biological sink of the greenhouse gas methane in marine sediments (doi:10.1007/978-94-009-0213-8_44) and serves as an important control for emission of methane into the hydrosphere. The AOM metabolic process is assumed to be a reversal of methanogenesis coupled to the reduction of sulfate to sulfide involving methanotrophic archaea (ANME) and sulfate-reducing bacteria (SRB) as syntrophic partners which were describes amongst others in Boetius et al. (2000; doi:10.1038/35036572). In this study, 16S rRNA-based methods were used to investigate the distribution and biomass of archaea in samples from sediments above outcropping methane hydrate at Hydrate Ridge (Cascadia margin off Oregon) and (ii) massive microbial mats enclosing carbonate reefs (Crimea area, Black Sea). Sediment samples from Hydrate Ridge were obtained during R/V SONNE cruises SO143-2 in August 1999 and SO148-1 in August 2000 at the crest of southern Hydrate Ridge at the Cascadia convergent margin off the coast of Oregon. The second study area is located in the Black Sea and represents a field in which there is active seepage of free gas on the slope of the northwestern Crimea area. Here, a field of conspicuous microbial reefs forming chimney-like structures was discovered at a water depth of 230 m in anoxic waters. The microbial mats were sampled by using the manned submersible JAGO during the R/V Prof. LOGACHEV cruise in July 2001. At Hydrate Ridge the surface sediments were dominated by aggregates consisting of ANME-2 and members of the Desulfosarcina-Desulfococcus branch (DSS) (ANME-2/DSS aggregates), which accounted for >90% of the total cell biomass. The numbers of ANME-1 cells increased strongly with depth; these cells accounted 1% of all single cells at the surface and more than 30% of all single cells (5% of the total cells) in 7- to 10-cm sediment horizons that were directly above layers of gas hydrate. In the Black Sea microbial mats ANME-1 accounted for about ...