Culture conditions, mass spectrometric measurements and acclimation carbonate chemistry
- A combined increase in seawater [CO2] and [H+] was recently shown to induce a shift from photosynthetic HCO3- to CO2 uptake in Emiliania huxleyi. This shift occurred within minutes, whereas acclimation to ocean acidification (OA) did not affect the carbon source. - To identify the driver of this s...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.859864 2023-05-15T17:51:20+02:00 Culture conditions, mass spectrometric measurements and acclimation carbonate chemistry Kottmeier, Dorothee Rokitta, Sebastian D Rost, Björn 2016-04-20 application/zip, 16.7 kBytes https://doi.pangaea.de/10.1594/PANGAEA.859864 https://doi.org/10.1594/PANGAEA.859864 en eng PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.859864 https://doi.org/10.1594/PANGAEA.859864 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Kottmeier, Dorothee; Rokitta, Sebastian D; Rost, Björn (2016): Acidification, not carbonation, is the major regulator of carbon fluxes in the coccolithophore Emiliania huxleyi. New Phytologist, 211(1), 126-137, https://doi.org/10.1111/nph.13885 AWI_Phytochange Phytochange @ AWI Dataset 2016 ftpangaea https://doi.org/10.1594/PANGAEA.859864 https://doi.org/10.1111/nph.13885 2023-01-20T09:07:10Z - A combined increase in seawater [CO2] and [H+] was recently shown to induce a shift from photosynthetic HCO3- to CO2 uptake in Emiliania huxleyi. This shift occurred within minutes, whereas acclimation to ocean acidification (OA) did not affect the carbon source. - To identify the driver of this shift, we exposed low- and high-light acclimated E. huxleyi to a matrix of two levels of dissolved inorganic carbon (1400, 2800 lmol kg-1) and pH (8.15, 7.85) and directly measured cellular O2, CO2 and HCO3 fluxes under these conditions. - Exposure to increased [CO2] had little effect on the photosynthetic fluxes, whereas increased [H+] led to a significant decline in HCO3- uptake. Low-light acclimated cells overcompensated for the inhibition of HCO3- uptake by increasing CO2 uptake. High-light acclimated cells, relying on higher proportions of HCO3- uptake, could not increase CO2 uptake and photosynthetic O2 evolution consequently became carbon-limited. - These regulations indicate that OA responses in photosynthesis are caused by [H+] rather than by [CO2]. The impaired HCO3- uptake also provides a mechanistic explanation for lowered calcification under OA. Moreover, it explains the OA-dependent decrease in photosynthesis observed in high-light grown phytoplankton. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
AWI_Phytochange Phytochange @ AWI |
spellingShingle |
AWI_Phytochange Phytochange @ AWI Kottmeier, Dorothee Rokitta, Sebastian D Rost, Björn Culture conditions, mass spectrometric measurements and acclimation carbonate chemistry |
topic_facet |
AWI_Phytochange Phytochange @ AWI |
description |
- A combined increase in seawater [CO2] and [H+] was recently shown to induce a shift from photosynthetic HCO3- to CO2 uptake in Emiliania huxleyi. This shift occurred within minutes, whereas acclimation to ocean acidification (OA) did not affect the carbon source. - To identify the driver of this shift, we exposed low- and high-light acclimated E. huxleyi to a matrix of two levels of dissolved inorganic carbon (1400, 2800 lmol kg-1) and pH (8.15, 7.85) and directly measured cellular O2, CO2 and HCO3 fluxes under these conditions. - Exposure to increased [CO2] had little effect on the photosynthetic fluxes, whereas increased [H+] led to a significant decline in HCO3- uptake. Low-light acclimated cells overcompensated for the inhibition of HCO3- uptake by increasing CO2 uptake. High-light acclimated cells, relying on higher proportions of HCO3- uptake, could not increase CO2 uptake and photosynthetic O2 evolution consequently became carbon-limited. - These regulations indicate that OA responses in photosynthesis are caused by [H+] rather than by [CO2]. The impaired HCO3- uptake also provides a mechanistic explanation for lowered calcification under OA. Moreover, it explains the OA-dependent decrease in photosynthesis observed in high-light grown phytoplankton. |
format |
Dataset |
author |
Kottmeier, Dorothee Rokitta, Sebastian D Rost, Björn |
author_facet |
Kottmeier, Dorothee Rokitta, Sebastian D Rost, Björn |
author_sort |
Kottmeier, Dorothee |
title |
Culture conditions, mass spectrometric measurements and acclimation carbonate chemistry |
title_short |
Culture conditions, mass spectrometric measurements and acclimation carbonate chemistry |
title_full |
Culture conditions, mass spectrometric measurements and acclimation carbonate chemistry |
title_fullStr |
Culture conditions, mass spectrometric measurements and acclimation carbonate chemistry |
title_full_unstemmed |
Culture conditions, mass spectrometric measurements and acclimation carbonate chemistry |
title_sort |
culture conditions, mass spectrometric measurements and acclimation carbonate chemistry |
publisher |
PANGAEA |
publishDate |
2016 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.859864 https://doi.org/10.1594/PANGAEA.859864 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Supplement to: Kottmeier, Dorothee; Rokitta, Sebastian D; Rost, Björn (2016): Acidification, not carbonation, is the major regulator of carbon fluxes in the coccolithophore Emiliania huxleyi. New Phytologist, 211(1), 126-137, https://doi.org/10.1111/nph.13885 |
op_relation |
https://doi.pangaea.de/10.1594/PANGAEA.859864 https://doi.org/10.1594/PANGAEA.859864 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.859864 https://doi.org/10.1111/nph.13885 |
_version_ |
1766158451429343232 |