Enzyme activity, body measures and heart mass of Sepia officinalis sampled in the English Channel and Adriatic Sea
In the eurythermal cuttlefish Sepia officinalis, performance depends on hearts that ensure systemic oxygen supply over a broad range of temperatures. We therefore aimed to identify adjustments in energetic cardiac capacity and underlying mitochondrial function supporting thermal acclimation and adap...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.848989 2023-05-15T13:44:47+02:00 Enzyme activity, body measures and heart mass of Sepia officinalis sampled in the English Channel and Adriatic Sea Oellermann, Michael Pörtner, Hans-Otto Mark, Felix Christopher MEDIAN LATITUDE: 48.400000 * MEDIAN LONGITUDE: 8.116665 * SOUTH-BOUND LATITUDE: 45.216670 * WEST-BOUND LONGITUDE: 3.933330 * NORTH-BOUND LATITUDE: 51.583330 * EAST-BOUND LONGITUDE: 12.300000 * DATE/TIME START: 2009-06-01T00:00:00 * DATE/TIME END: 2010-11-22T00:00:00 2011-08-31 application/zip, 2 datasets https://doi.pangaea.de/10.1594/PANGAEA.848989 https://doi.org/10.1594/PANGAEA.848989 en eng PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.848989 https://doi.org/10.1594/PANGAEA.848989 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Oellermann, Michael; Pörtner, Hans-Otto; Mark, Felix Christopher (2012): Mitochondrial dynamics underlying thermal plasticity of cuttlefish (Sepia officinalis) hearts. Journal of Experimental Biology, 215(17), 2992-3000, https://doi.org/10.1242/jeb.068163 Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158 Dataset 2011 ftpangaea https://doi.org/10.1594/PANGAEA.848989 https://doi.org/10.1242/jeb.068163 2023-01-20T07:33:30Z In the eurythermal cuttlefish Sepia officinalis, performance depends on hearts that ensure systemic oxygen supply over a broad range of temperatures. We therefore aimed to identify adjustments in energetic cardiac capacity and underlying mitochondrial function supporting thermal acclimation and adaptation that could be crucial for the cuttlefish's competitive success in variable environments. Two genetically distinct cuttlefish populations were acclimated to 11, 16 and 21°C. Subsequently, skinned and permeabilised heart fibres were used to assess mitochondrial functioning by means of high-resolution respirometry and a substrate-inhibitor protocol, followed by measurements of cardiac citrate synthase and cytosolic enzyme activities. Temperate English Channel cuttlefish had lower mitochondrial capacities but larger hearts than subtropical Adriatic cuttlefish. Warm acclimation to 21°C decreased mitochondrial complex I activity in Adriatic cuttlefish and increased complex IV activity in English Channel cuttlefish. However, compensation of mitochondrial capacities did not occur during cold acclimation to 11°C. In systemic hearts, the thermal sensitivity of mitochondrial substrate oxidation was high for proline and pyruvate but low for succinate. Oxygen efficiency of catabolism rose as temperature changed from 11 to 21°C via shifts to oxygen-conserving oxidation of proline and pyruvate and via reduced relative proton leak. The changes observed for substrate oxidation, mitochondrial complexes, relative proton leak and heart mass improve energetic efficiency and essentially seem to extend tolerance to high temperatures and reduce associated tissue hypoxia. We conclude that cuttlefish sustain cardiac performance and, thus, systemic oxygen delivery over short- and long-term changes of temperature and environmental conditions by multiple adjustments in cellular and mitochondrial energetics. Dataset Antarc* Antarctic Arctic Sea ice PANGAEA - Data Publisher for Earth & Environmental Science Antarctic Arctic ENVELOPE(3.933330,12.300000,51.583330,45.216670) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158 |
spellingShingle |
Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158 Oellermann, Michael Pörtner, Hans-Otto Mark, Felix Christopher Enzyme activity, body measures and heart mass of Sepia officinalis sampled in the English Channel and Adriatic Sea |
topic_facet |
Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158 |
description |
In the eurythermal cuttlefish Sepia officinalis, performance depends on hearts that ensure systemic oxygen supply over a broad range of temperatures. We therefore aimed to identify adjustments in energetic cardiac capacity and underlying mitochondrial function supporting thermal acclimation and adaptation that could be crucial for the cuttlefish's competitive success in variable environments. Two genetically distinct cuttlefish populations were acclimated to 11, 16 and 21°C. Subsequently, skinned and permeabilised heart fibres were used to assess mitochondrial functioning by means of high-resolution respirometry and a substrate-inhibitor protocol, followed by measurements of cardiac citrate synthase and cytosolic enzyme activities. Temperate English Channel cuttlefish had lower mitochondrial capacities but larger hearts than subtropical Adriatic cuttlefish. Warm acclimation to 21°C decreased mitochondrial complex I activity in Adriatic cuttlefish and increased complex IV activity in English Channel cuttlefish. However, compensation of mitochondrial capacities did not occur during cold acclimation to 11°C. In systemic hearts, the thermal sensitivity of mitochondrial substrate oxidation was high for proline and pyruvate but low for succinate. Oxygen efficiency of catabolism rose as temperature changed from 11 to 21°C via shifts to oxygen-conserving oxidation of proline and pyruvate and via reduced relative proton leak. The changes observed for substrate oxidation, mitochondrial complexes, relative proton leak and heart mass improve energetic efficiency and essentially seem to extend tolerance to high temperatures and reduce associated tissue hypoxia. We conclude that cuttlefish sustain cardiac performance and, thus, systemic oxygen delivery over short- and long-term changes of temperature and environmental conditions by multiple adjustments in cellular and mitochondrial energetics. |
format |
Dataset |
author |
Oellermann, Michael Pörtner, Hans-Otto Mark, Felix Christopher |
author_facet |
Oellermann, Michael Pörtner, Hans-Otto Mark, Felix Christopher |
author_sort |
Oellermann, Michael |
title |
Enzyme activity, body measures and heart mass of Sepia officinalis sampled in the English Channel and Adriatic Sea |
title_short |
Enzyme activity, body measures and heart mass of Sepia officinalis sampled in the English Channel and Adriatic Sea |
title_full |
Enzyme activity, body measures and heart mass of Sepia officinalis sampled in the English Channel and Adriatic Sea |
title_fullStr |
Enzyme activity, body measures and heart mass of Sepia officinalis sampled in the English Channel and Adriatic Sea |
title_full_unstemmed |
Enzyme activity, body measures and heart mass of Sepia officinalis sampled in the English Channel and Adriatic Sea |
title_sort |
enzyme activity, body measures and heart mass of sepia officinalis sampled in the english channel and adriatic sea |
publisher |
PANGAEA |
publishDate |
2011 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.848989 https://doi.org/10.1594/PANGAEA.848989 |
op_coverage |
MEDIAN LATITUDE: 48.400000 * MEDIAN LONGITUDE: 8.116665 * SOUTH-BOUND LATITUDE: 45.216670 * WEST-BOUND LONGITUDE: 3.933330 * NORTH-BOUND LATITUDE: 51.583330 * EAST-BOUND LONGITUDE: 12.300000 * DATE/TIME START: 2009-06-01T00:00:00 * DATE/TIME END: 2010-11-22T00:00:00 |
long_lat |
ENVELOPE(3.933330,12.300000,51.583330,45.216670) |
geographic |
Antarctic Arctic |
geographic_facet |
Antarctic Arctic |
genre |
Antarc* Antarctic Arctic Sea ice |
genre_facet |
Antarc* Antarctic Arctic Sea ice |
op_source |
Supplement to: Oellermann, Michael; Pörtner, Hans-Otto; Mark, Felix Christopher (2012): Mitochondrial dynamics underlying thermal plasticity of cuttlefish (Sepia officinalis) hearts. Journal of Experimental Biology, 215(17), 2992-3000, https://doi.org/10.1242/jeb.068163 |
op_relation |
https://doi.pangaea.de/10.1594/PANGAEA.848989 https://doi.org/10.1594/PANGAEA.848989 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.848989 https://doi.org/10.1242/jeb.068163 |
_version_ |
1766206337260191744 |