Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua)
Effects of severe hypercapnia have been extensively studied in marine fishes, while knowledge on the impacts of moderately elevated CO2 levels and their combination with warming is scarce. Here we investigate ion regulation mechanisms and energy budget in gills from Atlantic cod acclimated long-term...
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Language: | English |
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PANGAEA
2015
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Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.848599 https://doi.org/10.1594/PANGAEA.848599 |
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.848599 |
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openpolar |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Adenosine triphosphatase activity Alkalinity total Animalia Aragonite saturation state Bicarbonate ion BIOACID Biological Impacts of Ocean Acidification Calcite saturation state Calculated Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved standard deviation Carbonate ion Carbonate system computation flag Carbon dioxide Chordata Coast and continental shelf Concentration Containers and aquaria (20-1000 L or < 1 m**2) CTD SEA-BIRD SBE 911plus Description Enzyme Figure Fraction Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gadus morhua Laboratory experiment Nekton North Atlantic OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Respiration Respiration rate oxygen |
spellingShingle |
Adenosine triphosphatase activity Alkalinity total Animalia Aragonite saturation state Bicarbonate ion BIOACID Biological Impacts of Ocean Acidification Calcite saturation state Calculated Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved standard deviation Carbonate ion Carbonate system computation flag Carbon dioxide Chordata Coast and continental shelf Concentration Containers and aquaria (20-1000 L or < 1 m**2) CTD SEA-BIRD SBE 911plus Description Enzyme Figure Fraction Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gadus morhua Laboratory experiment Nekton North Atlantic OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Respiration Respiration rate oxygen Kreiss, Cornelia M Michael, Katharina Bock, Christian Lucassen, Magnus Pörtner, Hans-Otto Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) |
topic_facet |
Adenosine triphosphatase activity Alkalinity total Animalia Aragonite saturation state Bicarbonate ion BIOACID Biological Impacts of Ocean Acidification Calcite saturation state Calculated Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved standard deviation Carbonate ion Carbonate system computation flag Carbon dioxide Chordata Coast and continental shelf Concentration Containers and aquaria (20-1000 L or < 1 m**2) CTD SEA-BIRD SBE 911plus Description Enzyme Figure Fraction Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gadus morhua Laboratory experiment Nekton North Atlantic OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Respiration Respiration rate oxygen |
description |
Effects of severe hypercapnia have been extensively studied in marine fishes, while knowledge on the impacts of moderately elevated CO2 levels and their combination with warming is scarce. Here we investigate ion regulation mechanisms and energy budget in gills from Atlantic cod acclimated long-term to elevated PCO2 levels (2500 µatm) and temperature (18 °C). Isolated perfused gill preparations established to determine gill thermal plasticity during acute exposures (10-22 °C) and in vivo costs of Na+/K+-ATPase activity, protein and RNA synthesis. Maximum enzyme capacities of F1Fo-ATPase, H+-ATPase and Na+/K+-ATPase were measured in vitro in crude gill homogenates. After whole animal acclimation to elevated PCO2 and/or warming, branchial oxygen consumption responded more strongly to acute temperature change. The fractions of gill respiration allocated to protein and RNA synthesis remained unchanged. In gills of fish CO2-exposed at both temperatures, energy turnover associated with Na+/K+-ATPase activity was reduced by 30% below rates of control fish. This contrasted in vitro capacities of Na+/K+-ATPase, which remained unchanged under elevated CO2 at 10 °C, and earlier studies which had found a strong upregulation under severe hypercapnia. F1Fo-ATPase capacities increased in hypercapnic gills at both temperatures, whereas Na+/K+ATPase and H+-ATPase capacities only increased in response to elevated CO2 and warming indicating the absence of thermal compensation under CO2. We conclude that in vivo ion regulatory energy demand is lowered under moderately elevated CO2 levels despite the stronger thermal response of total gill respiration and the upregulation of F1Fo-ATPase. This effect is maintained at elevated temperature. |
format |
Dataset |
author |
Kreiss, Cornelia M Michael, Katharina Bock, Christian Lucassen, Magnus Pörtner, Hans-Otto |
author_facet |
Kreiss, Cornelia M Michael, Katharina Bock, Christian Lucassen, Magnus Pörtner, Hans-Otto |
author_sort |
Kreiss, Cornelia M |
title |
Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) |
title_short |
Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) |
title_full |
Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) |
title_fullStr |
Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) |
title_full_unstemmed |
Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) |
title_sort |
impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of atlantic cod (gadus morhua) |
publisher |
PANGAEA |
publishDate |
2015 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.848599 https://doi.org/10.1594/PANGAEA.848599 |
genre |
atlantic cod Gadus morhua North Atlantic Ocean acidification |
genre_facet |
atlantic cod Gadus morhua North Atlantic Ocean acidification |
op_source |
Supplement to: Kreiss, Cornelia M; Michael, Katharina; Bock, Christian; Lucassen, Magnus; Pörtner, Hans-Otto (2015): Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills of Atlantic cod (Gadus morhua). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 182, 102-112, https://doi.org/10.1016/j.cbpa.2014.12.019 |
op_relation |
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.848599 https://doi.org/10.1594/PANGAEA.848599 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.848599 https://doi.org/10.1016/j.cbpa.2014.12.019 |
_version_ |
1766357976249008128 |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.848599 2023-05-15T15:27:33+02:00 Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) Kreiss, Cornelia M Michael, Katharina Bock, Christian Lucassen, Magnus Pörtner, Hans-Otto 2015-08-06 text/tab-separated-values, 2064 data points https://doi.pangaea.de/10.1594/PANGAEA.848599 https://doi.org/10.1594/PANGAEA.848599 en eng PANGAEA Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.848599 https://doi.org/10.1594/PANGAEA.848599 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Kreiss, Cornelia M; Michael, Katharina; Bock, Christian; Lucassen, Magnus; Pörtner, Hans-Otto (2015): Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills of Atlantic cod (Gadus morhua). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 182, 102-112, https://doi.org/10.1016/j.cbpa.2014.12.019 Adenosine triphosphatase activity Alkalinity total Animalia Aragonite saturation state Bicarbonate ion BIOACID Biological Impacts of Ocean Acidification Calcite saturation state Calculated Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved standard deviation Carbonate ion Carbonate system computation flag Carbon dioxide Chordata Coast and continental shelf Concentration Containers and aquaria (20-1000 L or < 1 m**2) CTD SEA-BIRD SBE 911plus Description Enzyme Figure Fraction Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gadus morhua Laboratory experiment Nekton North Atlantic OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Respiration Respiration rate oxygen Dataset 2015 ftpangaea https://doi.org/10.1594/PANGAEA.848599 https://doi.org/10.1016/j.cbpa.2014.12.019 2023-01-20T09:06:12Z Effects of severe hypercapnia have been extensively studied in marine fishes, while knowledge on the impacts of moderately elevated CO2 levels and their combination with warming is scarce. Here we investigate ion regulation mechanisms and energy budget in gills from Atlantic cod acclimated long-term to elevated PCO2 levels (2500 µatm) and temperature (18 °C). Isolated perfused gill preparations established to determine gill thermal plasticity during acute exposures (10-22 °C) and in vivo costs of Na+/K+-ATPase activity, protein and RNA synthesis. Maximum enzyme capacities of F1Fo-ATPase, H+-ATPase and Na+/K+-ATPase were measured in vitro in crude gill homogenates. After whole animal acclimation to elevated PCO2 and/or warming, branchial oxygen consumption responded more strongly to acute temperature change. The fractions of gill respiration allocated to protein and RNA synthesis remained unchanged. In gills of fish CO2-exposed at both temperatures, energy turnover associated with Na+/K+-ATPase activity was reduced by 30% below rates of control fish. This contrasted in vitro capacities of Na+/K+-ATPase, which remained unchanged under elevated CO2 at 10 °C, and earlier studies which had found a strong upregulation under severe hypercapnia. F1Fo-ATPase capacities increased in hypercapnic gills at both temperatures, whereas Na+/K+ATPase and H+-ATPase capacities only increased in response to elevated CO2 and warming indicating the absence of thermal compensation under CO2. We conclude that in vivo ion regulatory energy demand is lowered under moderately elevated CO2 levels despite the stronger thermal response of total gill respiration and the upregulation of F1Fo-ATPase. This effect is maintained at elevated temperature. Dataset atlantic cod Gadus morhua North Atlantic Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |