Lipid, carbon and nitrogen content and fatty acid composition of Temora longicornis females and different food species during the experiment
Temora longicornis, a dominant calanoid copepod species in the North Sea, is characterised by low lipid reserves and high biomass turnover rates. To survive and reproduce successfully, this species needs continuous food supply and thus requires a highly flexible digestive system to exploit various f...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.848501 2024-09-09T19:02:56+00:00 Lipid, carbon and nitrogen content and fatty acid composition of Temora longicornis females and different food species during the experiment Kreibich, Tobias Saborowski, Reinhard Hagen, Wilhelm Niehoff, Barbara 2011 application/zip, 2 datasets https://doi.pangaea.de/10.1594/PANGAEA.848501 https://doi.org/10.1594/PANGAEA.848501 en eng PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.848501 https://doi.org/10.1594/PANGAEA.848501 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Kreibich, Tobias; Saborowski, Reinhard; Hagen, Wilhelm; Niehoff, Barbara (2011): Influence of short-term nutritional variations on digestive enzyme and fatty acid patterns of the calanoid copepod Temora longicornis. Journal of Experimental Marine Biology and Ecology, 407(2), 182-189, https://doi.org/10.1016/j.jembe.2011.06.013 AWI Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158 dataset publication series 2011 ftpangaea https://doi.org/10.1594/PANGAEA.84850110.1016/j.jembe.2011.06.013 2024-07-24T02:31:21Z Temora longicornis, a dominant calanoid copepod species in the North Sea, is characterised by low lipid reserves and high biomass turnover rates. To survive and reproduce successfully, this species needs continuous food supply and thus requires a highly flexible digestive system to exploit various food sources. Information on the capacity of digestive enzymes is scarce and therefore the aim of our study was to investigate the enzymatic capability to respond to quickly changing nutritional conditions. We conducted two feeding experiments with female T. longicornis from the southern North Sea off Helgoland. In the first experiment in 2005, we tested how digestive enzyme activities and enzyme patterns as revealed by substrate SDS-PAGE (sodium dodecylsulfate polyacrylamide gel electrophoresis) responded to changes in food composition. Females were incubated for three days fed ad libitum with either the heterotrophic dinoflagellate Oxyrrhis marina or the diatom Thalassiosira weissflogii. At the beginning and at the end of the experiment, copepods were deep-frozen for analyses. The lipolytic enzyme activity did not change over the course of the experiment but the enzyme patterns did, indicating a distinct diet-induced response. In a second experiment in 2008, we therefore focused on the enzyme patterns, testing how fast changes occur and whether feeding on the same algal species leads to similar patterns. In this experiment, we kept the females for 4 days at surplus food while changing the algal food species daily. At day 1, copepods were offered O. marina. On day 2, females received the cryptophycean Rhodomonas baltica followed by T. weissflogii on day 3. On day 4 copepods were again fed with O. marina. Each day, copepods were frozen for analysis by means of substrate SDS-PAGE. This showed that within 24 h new digestive enzymes appeared on the electrophoresis gels while others disappeared with the introduction of a new food species, and that the patterns were similar on day 1 and 4, when females were fed with O. ... Other/Unknown Material Antarc* Antarctic Arctic Sea ice Copepods PANGAEA - Data Publisher for Earth & Environmental Science Antarctic Arctic Helgoland |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
AWI Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158 |
spellingShingle |
AWI Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158 Kreibich, Tobias Saborowski, Reinhard Hagen, Wilhelm Niehoff, Barbara Lipid, carbon and nitrogen content and fatty acid composition of Temora longicornis females and different food species during the experiment |
topic_facet |
AWI Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158 |
description |
Temora longicornis, a dominant calanoid copepod species in the North Sea, is characterised by low lipid reserves and high biomass turnover rates. To survive and reproduce successfully, this species needs continuous food supply and thus requires a highly flexible digestive system to exploit various food sources. Information on the capacity of digestive enzymes is scarce and therefore the aim of our study was to investigate the enzymatic capability to respond to quickly changing nutritional conditions. We conducted two feeding experiments with female T. longicornis from the southern North Sea off Helgoland. In the first experiment in 2005, we tested how digestive enzyme activities and enzyme patterns as revealed by substrate SDS-PAGE (sodium dodecylsulfate polyacrylamide gel electrophoresis) responded to changes in food composition. Females were incubated for three days fed ad libitum with either the heterotrophic dinoflagellate Oxyrrhis marina or the diatom Thalassiosira weissflogii. At the beginning and at the end of the experiment, copepods were deep-frozen for analyses. The lipolytic enzyme activity did not change over the course of the experiment but the enzyme patterns did, indicating a distinct diet-induced response. In a second experiment in 2008, we therefore focused on the enzyme patterns, testing how fast changes occur and whether feeding on the same algal species leads to similar patterns. In this experiment, we kept the females for 4 days at surplus food while changing the algal food species daily. At day 1, copepods were offered O. marina. On day 2, females received the cryptophycean Rhodomonas baltica followed by T. weissflogii on day 3. On day 4 copepods were again fed with O. marina. Each day, copepods were frozen for analysis by means of substrate SDS-PAGE. This showed that within 24 h new digestive enzymes appeared on the electrophoresis gels while others disappeared with the introduction of a new food species, and that the patterns were similar on day 1 and 4, when females were fed with O. ... |
format |
Other/Unknown Material |
author |
Kreibich, Tobias Saborowski, Reinhard Hagen, Wilhelm Niehoff, Barbara |
author_facet |
Kreibich, Tobias Saborowski, Reinhard Hagen, Wilhelm Niehoff, Barbara |
author_sort |
Kreibich, Tobias |
title |
Lipid, carbon and nitrogen content and fatty acid composition of Temora longicornis females and different food species during the experiment |
title_short |
Lipid, carbon and nitrogen content and fatty acid composition of Temora longicornis females and different food species during the experiment |
title_full |
Lipid, carbon and nitrogen content and fatty acid composition of Temora longicornis females and different food species during the experiment |
title_fullStr |
Lipid, carbon and nitrogen content and fatty acid composition of Temora longicornis females and different food species during the experiment |
title_full_unstemmed |
Lipid, carbon and nitrogen content and fatty acid composition of Temora longicornis females and different food species during the experiment |
title_sort |
lipid, carbon and nitrogen content and fatty acid composition of temora longicornis females and different food species during the experiment |
publisher |
PANGAEA |
publishDate |
2011 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.848501 https://doi.org/10.1594/PANGAEA.848501 |
geographic |
Antarctic Arctic Helgoland |
geographic_facet |
Antarctic Arctic Helgoland |
genre |
Antarc* Antarctic Arctic Sea ice Copepods |
genre_facet |
Antarc* Antarctic Arctic Sea ice Copepods |
op_source |
Supplement to: Kreibich, Tobias; Saborowski, Reinhard; Hagen, Wilhelm; Niehoff, Barbara (2011): Influence of short-term nutritional variations on digestive enzyme and fatty acid patterns of the calanoid copepod Temora longicornis. Journal of Experimental Marine Biology and Ecology, 407(2), 182-189, https://doi.org/10.1016/j.jembe.2011.06.013 |
op_relation |
https://doi.pangaea.de/10.1594/PANGAEA.848501 https://doi.org/10.1594/PANGAEA.848501 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.84850110.1016/j.jembe.2011.06.013 |
_version_ |
1809816951894048768 |