Adenylate concentration and energy charge in different thallus regions and after UV radiation in brown, red and green algae
A method was developed to extract adenine nucleotides AMP, ADP, and ATP from marine macroalgal tissue to gain information on the cellular energy charge. Quantification was carried out by high performance liquid chromatography (HPLC). Three species from the rocky shore of the island of Helgoland (Ger...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.848162 2024-09-09T19:06:46+00:00 Adenylate concentration and energy charge in different thallus regions and after UV radiation in brown, red and green algae Hünken, Mark Karsten, Ulf Wiencke, Christian 2005 application/zip, 2 datasets https://doi.pangaea.de/10.1594/PANGAEA.848162 https://doi.org/10.1594/PANGAEA.848162 en eng PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.848162 https://doi.org/10.1594/PANGAEA.848162 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Hünken, Mark; Karsten, Ulf; Wiencke, Christian (2005): Determination of the adenylate energy charge (AEC) as a tool to determine the physiological status of macroalgal tissues after UV exposure. Phycologia, 44(3), 249-253, https://doi.org/10.2216/0031-8884(2005)44%5B249:DOTAEC%5D2.0.CO;2 Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158 dataset publication series 2005 ftpangaea https://doi.org/10.1594/PANGAEA.84816210.2216/0031-8884(2005)44%5B249:DOTAEC%5D2.0.CO;2 2024-07-24T02:31:21Z A method was developed to extract adenine nucleotides AMP, ADP, and ATP from marine macroalgal tissue to gain information on the cellular energy charge. Quantification was carried out by high performance liquid chromatography (HPLC). Three species from the rocky shore of the island of Helgoland (German Bight) were examined: Laminaria saccharina (Phaeophyta), Chondrus crispus (Rhodophyta), and Ulva lactuca (Chlorophyta). In L. saccharina and C. crispus, the adenylate energy charge (AEC) was determined in different thallus regions. AEC varied in relation to tissue age and function. Higher AEC values typically occurred in thallus regions with meristematic activity. Furthermore, L. saccharina and U. lactuca were exposed to UV-A and elevated UV-B radiation. The AEC was calculated and the maximal quantum yield of photosystem II (Fv/Fm) was determined as indicators for UV stress. In both species, the AEC remained at high values (0.72 ± 0.04), while Fv/Fm dropped rapidly. The results show that the photosynthesis of the phaeophyte is more resistant to UV radiation than the chlorophyte. Other/Unknown Material Antarc* Antarctic Arctic Sea ice PANGAEA - Data Publisher for Earth & Environmental Science Antarctic Arctic Helgoland |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158 |
spellingShingle |
Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158 Hünken, Mark Karsten, Ulf Wiencke, Christian Adenylate concentration and energy charge in different thallus regions and after UV radiation in brown, red and green algae |
topic_facet |
Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158 |
description |
A method was developed to extract adenine nucleotides AMP, ADP, and ATP from marine macroalgal tissue to gain information on the cellular energy charge. Quantification was carried out by high performance liquid chromatography (HPLC). Three species from the rocky shore of the island of Helgoland (German Bight) were examined: Laminaria saccharina (Phaeophyta), Chondrus crispus (Rhodophyta), and Ulva lactuca (Chlorophyta). In L. saccharina and C. crispus, the adenylate energy charge (AEC) was determined in different thallus regions. AEC varied in relation to tissue age and function. Higher AEC values typically occurred in thallus regions with meristematic activity. Furthermore, L. saccharina and U. lactuca were exposed to UV-A and elevated UV-B radiation. The AEC was calculated and the maximal quantum yield of photosystem II (Fv/Fm) was determined as indicators for UV stress. In both species, the AEC remained at high values (0.72 ± 0.04), while Fv/Fm dropped rapidly. The results show that the photosynthesis of the phaeophyte is more resistant to UV radiation than the chlorophyte. |
format |
Other/Unknown Material |
author |
Hünken, Mark Karsten, Ulf Wiencke, Christian |
author_facet |
Hünken, Mark Karsten, Ulf Wiencke, Christian |
author_sort |
Hünken, Mark |
title |
Adenylate concentration and energy charge in different thallus regions and after UV radiation in brown, red and green algae |
title_short |
Adenylate concentration and energy charge in different thallus regions and after UV radiation in brown, red and green algae |
title_full |
Adenylate concentration and energy charge in different thallus regions and after UV radiation in brown, red and green algae |
title_fullStr |
Adenylate concentration and energy charge in different thallus regions and after UV radiation in brown, red and green algae |
title_full_unstemmed |
Adenylate concentration and energy charge in different thallus regions and after UV radiation in brown, red and green algae |
title_sort |
adenylate concentration and energy charge in different thallus regions and after uv radiation in brown, red and green algae |
publisher |
PANGAEA |
publishDate |
2005 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.848162 https://doi.org/10.1594/PANGAEA.848162 |
geographic |
Antarctic Arctic Helgoland |
geographic_facet |
Antarctic Arctic Helgoland |
genre |
Antarc* Antarctic Arctic Sea ice |
genre_facet |
Antarc* Antarctic Arctic Sea ice |
op_source |
Supplement to: Hünken, Mark; Karsten, Ulf; Wiencke, Christian (2005): Determination of the adenylate energy charge (AEC) as a tool to determine the physiological status of macroalgal tissues after UV exposure. Phycologia, 44(3), 249-253, https://doi.org/10.2216/0031-8884(2005)44%5B249:DOTAEC%5D2.0.CO;2 |
op_relation |
https://doi.pangaea.de/10.1594/PANGAEA.848162 https://doi.org/10.1594/PANGAEA.848162 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.84816210.2216/0031-8884(2005)44%5B249:DOTAEC%5D2.0.CO;2 |
_version_ |
1809820815105982464 |