Experimental ocean acidification alters the allocation of metabolic energy
Energy is required to maintain physiological homeostasis in response to environmental change. Although responses to environmental stressors frequently are assumed to involve high metabolic costs, the biochemical bases of actual energy demands are rarely quantified. We studied the impact of a near-fu...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.847832 2023-05-15T17:49:48+02:00 Experimental ocean acidification alters the allocation of metabolic energy Francis Pan, T C Applebaum, Scott L Manahan, Donal T 2015-07-08 text/tab-separated-values, 4876 data points https://doi.pangaea.de/10.1594/PANGAEA.847832 https://doi.org/10.1594/PANGAEA.847832 en eng PANGAEA Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.847832 https://doi.org/10.1594/PANGAEA.847832 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Francis Pan, T C; Applebaum, Scott L; Manahan, Donal T (2015): Experimental ocean acidification alters the allocation of metabolic energy. Proceedings of the National Academy of Sciences, 112(15), 4696-4701, https://doi.org/10.1073/pnas.1416967112 Age Alkalinity total standard error Animalia Aragonite saturation state Bicarbonate ion Body length standard deviation Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Containers and aquaria (20-1000 L or < 1 m**2) Coulometric titration Echinodermata Feeding mode Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Growth/Morphology In vivo Sodium Potassium adenosine triphosphatase activity per individual Laboratory experiment Not applicable OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos Percentage Dataset 2015 ftpangaea https://doi.org/10.1594/PANGAEA.847832 https://doi.org/10.1073/pnas.1416967112 2023-01-20T09:06:07Z Energy is required to maintain physiological homeostasis in response to environmental change. Although responses to environmental stressors frequently are assumed to involve high metabolic costs, the biochemical bases of actual energy demands are rarely quantified. We studied the impact of a near-future scenario of ocean acidification [800 µatm partial pressure of CO2 (pCO2)] during the development and growth of an important model organism in developmental and environmental biology, the sea urchin Strongylocentrotus purpuratus. Size, metabolic rate, biochemical content, and gene expression were not different in larvae growing under control and seawater acidification treatments. Measurements limited to those levels of biological analysis did not reveal the biochemical mechanisms of response to ocean acidification that occurred at the cellular level. In vivo rates of protein synthesis and ion transport increased 50% under acidification. Importantly, the in vivo physiological increases in ion transport were not predicted from total enzyme activity or gene expression. Under acidification, the increased rates of protein synthesis and ion transport that were sustained in growing larvae collectively accounted for the majority of available ATP (84%). In contrast, embryos and prefeeding and unfed larvae in control treatments allocated on average only 40% of ATP to these same two processes. Understanding the biochemical strategies for accommodating increases in metabolic energy demand and their biological limitations can serve as a quantitative basis for assessing sublethal effects of global change. Variation in the ability to allocate ATP differentially among essential functions may be a key basis of resilience to ocean acidification and other compounding environmental stressors. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Age Alkalinity total standard error Animalia Aragonite saturation state Bicarbonate ion Body length standard deviation Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Containers and aquaria (20-1000 L or < 1 m**2) Coulometric titration Echinodermata Feeding mode Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Growth/Morphology In vivo Sodium Potassium adenosine triphosphatase activity per individual Laboratory experiment Not applicable OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos Percentage |
spellingShingle |
Age Alkalinity total standard error Animalia Aragonite saturation state Bicarbonate ion Body length standard deviation Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Containers and aquaria (20-1000 L or < 1 m**2) Coulometric titration Echinodermata Feeding mode Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Growth/Morphology In vivo Sodium Potassium adenosine triphosphatase activity per individual Laboratory experiment Not applicable OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos Percentage Francis Pan, T C Applebaum, Scott L Manahan, Donal T Experimental ocean acidification alters the allocation of metabolic energy |
topic_facet |
Age Alkalinity total standard error Animalia Aragonite saturation state Bicarbonate ion Body length standard deviation Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Containers and aquaria (20-1000 L or < 1 m**2) Coulometric titration Echinodermata Feeding mode Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Growth/Morphology In vivo Sodium Potassium adenosine triphosphatase activity per individual Laboratory experiment Not applicable OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos Percentage |
description |
Energy is required to maintain physiological homeostasis in response to environmental change. Although responses to environmental stressors frequently are assumed to involve high metabolic costs, the biochemical bases of actual energy demands are rarely quantified. We studied the impact of a near-future scenario of ocean acidification [800 µatm partial pressure of CO2 (pCO2)] during the development and growth of an important model organism in developmental and environmental biology, the sea urchin Strongylocentrotus purpuratus. Size, metabolic rate, biochemical content, and gene expression were not different in larvae growing under control and seawater acidification treatments. Measurements limited to those levels of biological analysis did not reveal the biochemical mechanisms of response to ocean acidification that occurred at the cellular level. In vivo rates of protein synthesis and ion transport increased 50% under acidification. Importantly, the in vivo physiological increases in ion transport were not predicted from total enzyme activity or gene expression. Under acidification, the increased rates of protein synthesis and ion transport that were sustained in growing larvae collectively accounted for the majority of available ATP (84%). In contrast, embryos and prefeeding and unfed larvae in control treatments allocated on average only 40% of ATP to these same two processes. Understanding the biochemical strategies for accommodating increases in metabolic energy demand and their biological limitations can serve as a quantitative basis for assessing sublethal effects of global change. Variation in the ability to allocate ATP differentially among essential functions may be a key basis of resilience to ocean acidification and other compounding environmental stressors. |
format |
Dataset |
author |
Francis Pan, T C Applebaum, Scott L Manahan, Donal T |
author_facet |
Francis Pan, T C Applebaum, Scott L Manahan, Donal T |
author_sort |
Francis Pan, T C |
title |
Experimental ocean acidification alters the allocation of metabolic energy |
title_short |
Experimental ocean acidification alters the allocation of metabolic energy |
title_full |
Experimental ocean acidification alters the allocation of metabolic energy |
title_fullStr |
Experimental ocean acidification alters the allocation of metabolic energy |
title_full_unstemmed |
Experimental ocean acidification alters the allocation of metabolic energy |
title_sort |
experimental ocean acidification alters the allocation of metabolic energy |
publisher |
PANGAEA |
publishDate |
2015 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.847832 https://doi.org/10.1594/PANGAEA.847832 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Supplement to: Francis Pan, T C; Applebaum, Scott L; Manahan, Donal T (2015): Experimental ocean acidification alters the allocation of metabolic energy. Proceedings of the National Academy of Sciences, 112(15), 4696-4701, https://doi.org/10.1073/pnas.1416967112 |
op_relation |
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.847832 https://doi.org/10.1594/PANGAEA.847832 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.847832 https://doi.org/10.1073/pnas.1416967112 |
_version_ |
1766156276694253568 |