Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes
Corals play a key role in ocean ecosystems and carbonate balance, but their molecular response to ocean acidification remains unclear. The only previous whole-transcriptome study documented extensive disruption of gene expression, particularly of genes encoding skeletal organic matrix proteins, in j...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.847720 2023-05-15T17:51:12+02:00 Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes Moya, Aurélie Huisman, L Forêt, S Gattuso, Jean-Pierre Hayward, D C Ball, E E Miller, David J 2015-07-03 text/tab-separated-values, 568 data points https://doi.pangaea.de/10.1594/PANGAEA.847720 https://doi.org/10.1594/PANGAEA.847720 en eng PANGAEA Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.847720 https://doi.org/10.1594/PANGAEA.847720 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Moya, Aurélie; Huisman, L; Forêt, S; Gattuso, Jean-Pierre; Hayward, D C; Ball, E E; Miller, David J (2015): Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes. Molecular Ecology, 24(2), 438-452, https://doi.org/10.1111/mec.13021 Acropora millepora Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cnidaria Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Family Figure Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene abundance Gene expression (incl. proteomics) Group Laboratory experiment Number of sequences OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Percentage pH Potentiometric Salinity Single species South Pacific Species Temperature water Dataset 2015 ftpangaea https://doi.org/10.1594/PANGAEA.847720 https://doi.org/10.1111/mec.13021 2023-01-20T09:06:07Z Corals play a key role in ocean ecosystems and carbonate balance, but their molecular response to ocean acidification remains unclear. The only previous whole-transcriptome study documented extensive disruption of gene expression, particularly of genes encoding skeletal organic matrix proteins, in juvenile corals (Acropora millepora) after short-term (3 d) exposure to elevated pCO2. In this study, whole-transcriptome analysis was used to compare the effects of such 'acute' (3 d) exposure to elevated pCO2 with a longer ('prolonged'; 9 d) period of exposure beginning immediately post-fertilization. Far fewer genes were differentially expressed under the 9-d treatment, and although the transcriptome data implied wholesale disruption of metabolism and calcification genes in the acute treatment experiment, expression of most genes was at control levels after prolonged treatment. There was little overlap between the genes responding to the acute and prolonged treatments, but heat shock proteins (HSPs) and heat shock factors (HSFs) were over-represented amongst the genes responding to both treatments. Amongst these was an HSP70 gene previously shown to be involved in acclimation to thermal stress in a field population of another acroporid coral. The most obvious feature of the molecular response in the 9-d treatment experiment was the upregulation of five distinct Bcl-2 family members, the majority predicted to be anti-apoptotic. This suggests that an important component of the longer term response to elevated CO2 is suppression of apoptosis. It therefore appears that juvenile A. millepora have the capacity to rapidly acclimate to elevated pCO2, a process mediated by upregulation of specific HSPs and a suite of Bcl-2 family members. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science Pacific |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Acropora millepora Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cnidaria Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Family Figure Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene abundance Gene expression (incl. proteomics) Group Laboratory experiment Number of sequences OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Percentage pH Potentiometric Salinity Single species South Pacific Species Temperature water |
spellingShingle |
Acropora millepora Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cnidaria Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Family Figure Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene abundance Gene expression (incl. proteomics) Group Laboratory experiment Number of sequences OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Percentage pH Potentiometric Salinity Single species South Pacific Species Temperature water Moya, Aurélie Huisman, L Forêt, S Gattuso, Jean-Pierre Hayward, D C Ball, E E Miller, David J Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes |
topic_facet |
Acropora millepora Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cnidaria Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Family Figure Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene abundance Gene expression (incl. proteomics) Group Laboratory experiment Number of sequences OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Percentage pH Potentiometric Salinity Single species South Pacific Species Temperature water |
description |
Corals play a key role in ocean ecosystems and carbonate balance, but their molecular response to ocean acidification remains unclear. The only previous whole-transcriptome study documented extensive disruption of gene expression, particularly of genes encoding skeletal organic matrix proteins, in juvenile corals (Acropora millepora) after short-term (3 d) exposure to elevated pCO2. In this study, whole-transcriptome analysis was used to compare the effects of such 'acute' (3 d) exposure to elevated pCO2 with a longer ('prolonged'; 9 d) period of exposure beginning immediately post-fertilization. Far fewer genes were differentially expressed under the 9-d treatment, and although the transcriptome data implied wholesale disruption of metabolism and calcification genes in the acute treatment experiment, expression of most genes was at control levels after prolonged treatment. There was little overlap between the genes responding to the acute and prolonged treatments, but heat shock proteins (HSPs) and heat shock factors (HSFs) were over-represented amongst the genes responding to both treatments. Amongst these was an HSP70 gene previously shown to be involved in acclimation to thermal stress in a field population of another acroporid coral. The most obvious feature of the molecular response in the 9-d treatment experiment was the upregulation of five distinct Bcl-2 family members, the majority predicted to be anti-apoptotic. This suggests that an important component of the longer term response to elevated CO2 is suppression of apoptosis. It therefore appears that juvenile A. millepora have the capacity to rapidly acclimate to elevated pCO2, a process mediated by upregulation of specific HSPs and a suite of Bcl-2 family members. |
format |
Dataset |
author |
Moya, Aurélie Huisman, L Forêt, S Gattuso, Jean-Pierre Hayward, D C Ball, E E Miller, David J |
author_facet |
Moya, Aurélie Huisman, L Forêt, S Gattuso, Jean-Pierre Hayward, D C Ball, E E Miller, David J |
author_sort |
Moya, Aurélie |
title |
Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes |
title_short |
Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes |
title_full |
Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes |
title_fullStr |
Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes |
title_full_unstemmed |
Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes |
title_sort |
rapid acclimation of juvenile corals to co2-mediated acidification by upregulation of heat shock protein and bcl-2 genes |
publisher |
PANGAEA |
publishDate |
2015 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.847720 https://doi.org/10.1594/PANGAEA.847720 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Supplement to: Moya, Aurélie; Huisman, L; Forêt, S; Gattuso, Jean-Pierre; Hayward, D C; Ball, E E; Miller, David J (2015): Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes. Molecular Ecology, 24(2), 438-452, https://doi.org/10.1111/mec.13021 |
op_relation |
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.847720 https://doi.org/10.1594/PANGAEA.847720 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.847720 https://doi.org/10.1111/mec.13021 |
_version_ |
1766158273776451584 |