The effect of nitrate and phosphate availability on Emiliania huxleyi(NZEH) physiology under different CO2 scenarios
Growth and calcification of the marine coccolithophorid Emiliania huxleyi is affected by ocean acidification and macronutrients limitation and its response varies between strains. Here we investigated the physiological performance of a highly calcified E. huxleyi strain, NZEH, in a multiparametric e...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.842157 2024-09-15T18:28:00+00:00 The effect of nitrate and phosphate availability on Emiliania huxleyi(NZEH) physiology under different CO2 scenarios Rouco, Mónica Branson, O Lebrato, Mario Iglesias-Rodriguez, Debora 2013 text/tab-separated-values, 1422 data points https://doi.pangaea.de/10.1594/PANGAEA.842157 https://doi.org/10.1594/PANGAEA.842157 en eng PANGAEA Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0 [webpage]. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.842157 https://doi.org/10.1594/PANGAEA.842157 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Rouco, Mónica; Branson, O; Lebrato, Mario; Iglesias-Rodriguez, Debora (2013): The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios. Frontiers in Microbiology, 4, https://doi.org/10.3389/fmicb.2013.00155 Alkaline phosphatase para-Nitrophenylphosphate per cell Alkalinity total standard deviation Aragonite saturation state Bicarbonate Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chromista Coccoliths volume Coccosphere length Coulometric titration Emiliania huxleyi Figure Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Haptophyta Irradiance dataset 2013 ftpangaea https://doi.org/10.1594/PANGAEA.84215710.3389/fmicb.2013.00155 2024-07-24T02:31:33Z Growth and calcification of the marine coccolithophorid Emiliania huxleyi is affected by ocean acidification and macronutrients limitation and its response varies between strains. Here we investigated the physiological performance of a highly calcified E. huxleyi strain, NZEH, in a multiparametric experiment. Cells were exposed to different CO2 levels (ranging from 250 to 1314 µatm) under three nutrient conditions [nutrient replete (R), nitrate limited (-N), and phosphate limited (-P)]. We focused on calcite and organic carbon quotas and on nitrate and phosphate utilization by analyzing the activity of nitrate reductase (NRase) and alkaline phosphatase (APase), respectively. Particulate inorganic (PIC) and organic (POC) carbon quotas increased with increasing CO2 under R conditions but a different pattern was observed under nutrient limitation. The PIC:POC ratio decreased with increasing CO2 in nutrient limited cultures. Coccolith length increased with CO2 under all nutrient conditions but the coccosphere volume varied depending on the nutrient treatment. Maximum APase activity was found at 561 matm of CO2 (pH 7.92) in -P cultures and in R conditions, NRase activity increased linearly with CO2. These results suggest that E. huxleyi's competitive ability for nutrient uptake might be altered in future high-CO2 oceans. The combined dataset will be useful in model parameterizations of the carbon cycle and ocean acidification. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkaline phosphatase para-Nitrophenylphosphate per cell Alkalinity total standard deviation Aragonite saturation state Bicarbonate Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chromista Coccoliths volume Coccosphere length Coulometric titration Emiliania huxleyi Figure Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Haptophyta Irradiance |
spellingShingle |
Alkaline phosphatase para-Nitrophenylphosphate per cell Alkalinity total standard deviation Aragonite saturation state Bicarbonate Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chromista Coccoliths volume Coccosphere length Coulometric titration Emiliania huxleyi Figure Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Haptophyta Irradiance Rouco, Mónica Branson, O Lebrato, Mario Iglesias-Rodriguez, Debora The effect of nitrate and phosphate availability on Emiliania huxleyi(NZEH) physiology under different CO2 scenarios |
topic_facet |
Alkaline phosphatase para-Nitrophenylphosphate per cell Alkalinity total standard deviation Aragonite saturation state Bicarbonate Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chromista Coccoliths volume Coccosphere length Coulometric titration Emiliania huxleyi Figure Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Haptophyta Irradiance |
description |
Growth and calcification of the marine coccolithophorid Emiliania huxleyi is affected by ocean acidification and macronutrients limitation and its response varies between strains. Here we investigated the physiological performance of a highly calcified E. huxleyi strain, NZEH, in a multiparametric experiment. Cells were exposed to different CO2 levels (ranging from 250 to 1314 µatm) under three nutrient conditions [nutrient replete (R), nitrate limited (-N), and phosphate limited (-P)]. We focused on calcite and organic carbon quotas and on nitrate and phosphate utilization by analyzing the activity of nitrate reductase (NRase) and alkaline phosphatase (APase), respectively. Particulate inorganic (PIC) and organic (POC) carbon quotas increased with increasing CO2 under R conditions but a different pattern was observed under nutrient limitation. The PIC:POC ratio decreased with increasing CO2 in nutrient limited cultures. Coccolith length increased with CO2 under all nutrient conditions but the coccosphere volume varied depending on the nutrient treatment. Maximum APase activity was found at 561 matm of CO2 (pH 7.92) in -P cultures and in R conditions, NRase activity increased linearly with CO2. These results suggest that E. huxleyi's competitive ability for nutrient uptake might be altered in future high-CO2 oceans. The combined dataset will be useful in model parameterizations of the carbon cycle and ocean acidification. |
format |
Dataset |
author |
Rouco, Mónica Branson, O Lebrato, Mario Iglesias-Rodriguez, Debora |
author_facet |
Rouco, Mónica Branson, O Lebrato, Mario Iglesias-Rodriguez, Debora |
author_sort |
Rouco, Mónica |
title |
The effect of nitrate and phosphate availability on Emiliania huxleyi(NZEH) physiology under different CO2 scenarios |
title_short |
The effect of nitrate and phosphate availability on Emiliania huxleyi(NZEH) physiology under different CO2 scenarios |
title_full |
The effect of nitrate and phosphate availability on Emiliania huxleyi(NZEH) physiology under different CO2 scenarios |
title_fullStr |
The effect of nitrate and phosphate availability on Emiliania huxleyi(NZEH) physiology under different CO2 scenarios |
title_full_unstemmed |
The effect of nitrate and phosphate availability on Emiliania huxleyi(NZEH) physiology under different CO2 scenarios |
title_sort |
effect of nitrate and phosphate availability on emiliania huxleyi(nzeh) physiology under different co2 scenarios |
publisher |
PANGAEA |
publishDate |
2013 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.842157 https://doi.org/10.1594/PANGAEA.842157 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Supplement to: Rouco, Mónica; Branson, O; Lebrato, Mario; Iglesias-Rodriguez, Debora (2013): The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios. Frontiers in Microbiology, 4, https://doi.org/10.3389/fmicb.2013.00155 |
op_relation |
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0 [webpage]. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.842157 https://doi.org/10.1594/PANGAEA.842157 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.84215710.3389/fmicb.2013.00155 |
_version_ |
1810469300923793408 |