Effects of increased CO2 on fish gill and plasma proteome
Ocean acidification and warming are both primarily caused by increased levels of atmospheric CO2, and marine organisms are exposed to these two stressors simultaneously. Although the effects of temperature on fish have been investigated over the last century, the long-term effects of moderate CO2 ex...
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PANGAEA
2014
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Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.838003 https://doi.org/10.1594/PANGAEA.838003 |
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.838003 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Acanthopagrus schlegelii Accession number Acipenser baerii Alkalinity total Animalia Anoplopoma fimbria Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chordata Coast and continental shelf Comment Containers and aquaria (20-1000 L or < 1 m**2) Coturnix coturnix Danio rerio Dicentrarchus labrax Epinephelus bruneus Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Gillichthys mirabilis Hippoglossus hippoglossus Identification Laboratory experiment Larimichthys crocea Nekton North Atlantic OA-ICC Ocean Acidification International Coordination Centre Oncorhynchus mykiss Oreochromis mossambicus Paralichthys olivaceus Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos Peptide pH Platichthys flesus Potentiometric Potentiometric titration Protein name Protein spots |
spellingShingle |
Acanthopagrus schlegelii Accession number Acipenser baerii Alkalinity total Animalia Anoplopoma fimbria Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chordata Coast and continental shelf Comment Containers and aquaria (20-1000 L or < 1 m**2) Coturnix coturnix Danio rerio Dicentrarchus labrax Epinephelus bruneus Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Gillichthys mirabilis Hippoglossus hippoglossus Identification Laboratory experiment Larimichthys crocea Nekton North Atlantic OA-ICC Ocean Acidification International Coordination Centre Oncorhynchus mykiss Oreochromis mossambicus Paralichthys olivaceus Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos Peptide pH Platichthys flesus Potentiometric Potentiometric titration Protein name Protein spots Bresolin de Souza, Karine Jutfelt, Fredrik Kling, Peter Förlin, Lars Sturve, Joachim Hofmann, Gretchen E Effects of increased CO2 on fish gill and plasma proteome |
topic_facet |
Acanthopagrus schlegelii Accession number Acipenser baerii Alkalinity total Animalia Anoplopoma fimbria Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chordata Coast and continental shelf Comment Containers and aquaria (20-1000 L or < 1 m**2) Coturnix coturnix Danio rerio Dicentrarchus labrax Epinephelus bruneus Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Gillichthys mirabilis Hippoglossus hippoglossus Identification Laboratory experiment Larimichthys crocea Nekton North Atlantic OA-ICC Ocean Acidification International Coordination Centre Oncorhynchus mykiss Oreochromis mossambicus Paralichthys olivaceus Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos Peptide pH Platichthys flesus Potentiometric Potentiometric titration Protein name Protein spots |
description |
Ocean acidification and warming are both primarily caused by increased levels of atmospheric CO2, and marine organisms are exposed to these two stressors simultaneously. Although the effects of temperature on fish have been investigated over the last century, the long-term effects of moderate CO2 exposure and the combination of both stressors are almost entirely unknown. A proteomics approach was used to assess the adverse physiological and biochemical changes that may occur from the exposure to these two environmental stressors. We analysed gills and blood plasma of Atlantic halibut (Hippoglossus hippoglossus) exposed to temperatures of 12°C (control) and 18°C (impaired growth) in combination with control (400 µatm) or high-CO2 water (1000 µatm) for 14 weeks. The proteomic analysis was performed using two-dimensional gel electrophoresis (2DE) followed by Nanoflow LC-MS/MS using a LTQ-Orbitrap. The high-CO2 treatment induced the up-regulation of immune system-related proteins, as indicated by the up-regulation of the plasma proteins complement component C3 and fibrinogen beta chain precursor in both temperature treatments. Changes in gill proteome in the high-CO2 (18°C) group were mostly related to increased energy metabolism proteins (ATP synthase, malate dehydrogenase, malate dehydrogenase thermostable, and fructose-1,6-bisphosphate aldolase), possibly coupled to a higher energy demand. Gills from fish exposed to high-CO2 at both temperature treatments showed changes in proteins associated with increased cellular turnover and apoptosis signalling (annexin 5, eukaryotic translation elongation factor 1 gamma, receptor for protein kinase C, and putative ribosomal protein S27). This study indicates that moderate CO2-driven acidification, alone and combined with high temperature, can elicit biochemical changes that may affect fish health. |
format |
Dataset |
author |
Bresolin de Souza, Karine Jutfelt, Fredrik Kling, Peter Förlin, Lars Sturve, Joachim Hofmann, Gretchen E |
author_facet |
Bresolin de Souza, Karine Jutfelt, Fredrik Kling, Peter Förlin, Lars Sturve, Joachim Hofmann, Gretchen E |
author_sort |
Bresolin de Souza, Karine |
title |
Effects of increased CO2 on fish gill and plasma proteome |
title_short |
Effects of increased CO2 on fish gill and plasma proteome |
title_full |
Effects of increased CO2 on fish gill and plasma proteome |
title_fullStr |
Effects of increased CO2 on fish gill and plasma proteome |
title_full_unstemmed |
Effects of increased CO2 on fish gill and plasma proteome |
title_sort |
effects of increased co2 on fish gill and plasma proteome |
publisher |
PANGAEA |
publishDate |
2014 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.838003 https://doi.org/10.1594/PANGAEA.838003 |
genre |
Acipenser baerii North Atlantic Ocean acidification |
genre_facet |
Acipenser baerii North Atlantic Ocean acidification |
op_source |
Supplement to: Bresolin de Souza, Karine; Jutfelt, Fredrik; Kling, Peter; Förlin, Lars; Sturve, Joachim; Hofmann, Gretchen E (2014): Effects of Increased CO2 on Fish Gill and Plasma Proteome. PLoS ONE, 9(7), e102901, https://doi.org/10.1371/journal.pone.0102901 |
op_relation |
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.838003 https://doi.org/10.1594/PANGAEA.838003 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.838003 https://doi.org/10.1371/journal.pone.0102901 |
_version_ |
1766291763846184960 |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.838003 2023-05-15T13:01:59+02:00 Effects of increased CO2 on fish gill and plasma proteome Bresolin de Souza, Karine Jutfelt, Fredrik Kling, Peter Förlin, Lars Sturve, Joachim Hofmann, Gretchen E 2014-11-07 text/tab-separated-values, 792 data points https://doi.pangaea.de/10.1594/PANGAEA.838003 https://doi.org/10.1594/PANGAEA.838003 en eng PANGAEA Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.838003 https://doi.org/10.1594/PANGAEA.838003 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Bresolin de Souza, Karine; Jutfelt, Fredrik; Kling, Peter; Förlin, Lars; Sturve, Joachim; Hofmann, Gretchen E (2014): Effects of Increased CO2 on Fish Gill and Plasma Proteome. PLoS ONE, 9(7), e102901, https://doi.org/10.1371/journal.pone.0102901 Acanthopagrus schlegelii Accession number Acipenser baerii Alkalinity total Animalia Anoplopoma fimbria Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chordata Coast and continental shelf Comment Containers and aquaria (20-1000 L or < 1 m**2) Coturnix coturnix Danio rerio Dicentrarchus labrax Epinephelus bruneus Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Gillichthys mirabilis Hippoglossus hippoglossus Identification Laboratory experiment Larimichthys crocea Nekton North Atlantic OA-ICC Ocean Acidification International Coordination Centre Oncorhynchus mykiss Oreochromis mossambicus Paralichthys olivaceus Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos Peptide pH Platichthys flesus Potentiometric Potentiometric titration Protein name Protein spots Dataset 2014 ftpangaea https://doi.org/10.1594/PANGAEA.838003 https://doi.org/10.1371/journal.pone.0102901 2023-01-20T09:04:20Z Ocean acidification and warming are both primarily caused by increased levels of atmospheric CO2, and marine organisms are exposed to these two stressors simultaneously. Although the effects of temperature on fish have been investigated over the last century, the long-term effects of moderate CO2 exposure and the combination of both stressors are almost entirely unknown. A proteomics approach was used to assess the adverse physiological and biochemical changes that may occur from the exposure to these two environmental stressors. We analysed gills and blood plasma of Atlantic halibut (Hippoglossus hippoglossus) exposed to temperatures of 12°C (control) and 18°C (impaired growth) in combination with control (400 µatm) or high-CO2 water (1000 µatm) for 14 weeks. The proteomic analysis was performed using two-dimensional gel electrophoresis (2DE) followed by Nanoflow LC-MS/MS using a LTQ-Orbitrap. The high-CO2 treatment induced the up-regulation of immune system-related proteins, as indicated by the up-regulation of the plasma proteins complement component C3 and fibrinogen beta chain precursor in both temperature treatments. Changes in gill proteome in the high-CO2 (18°C) group were mostly related to increased energy metabolism proteins (ATP synthase, malate dehydrogenase, malate dehydrogenase thermostable, and fructose-1,6-bisphosphate aldolase), possibly coupled to a higher energy demand. Gills from fish exposed to high-CO2 at both temperature treatments showed changes in proteins associated with increased cellular turnover and apoptosis signalling (annexin 5, eukaryotic translation elongation factor 1 gamma, receptor for protein kinase C, and putative ribosomal protein S27). This study indicates that moderate CO2-driven acidification, alone and combined with high temperature, can elicit biochemical changes that may affect fish health. Dataset Acipenser baerii North Atlantic Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |