Adaptation of a globally important coccolithophore to ocean warming and acidification
Regulating intracellular pH (pHi) is critical for optimising the metabolic activity of corals, yet mechanisms involved in pH regulation and the buffering capacity within coral cells are not well understood. Our study investigated how the presence of symbiotic dinoflagellates affects the response of...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.837880 2023-05-15T17:52:09+02:00 Adaptation of a globally important coccolithophore to ocean warming and acidification Gibbin, Emma M Putnam, H M Davy, Simon K Gates, Ruth D 2014-11-04 text/tab-separated-values, 3840 data points https://doi.pangaea.de/10.1594/PANGAEA.837880 https://doi.org/10.1594/PANGAEA.837880 en eng PANGAEA Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.837880 https://doi.org/10.1594/PANGAEA.837880 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Gibbin, Emma M; Putnam, H M; Davy, Simon K; Gates, Ruth D (2014): Intracellular pH and its response to CO2-driven seawater acidification in symbiotic versus non-symbiotic coral cells. Journal of Experimental Biology, 217(11), 1963-1969, https://doi.org/10.1242/jeb.099549 Acid-base regulation Alkalinity total standard error Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cnidaria Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Figure Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH extracellular intracellular pH change Pocillopora damicornis Replicate Salinity Single species Species Temperature water Time in minutes Dataset 2014 ftpangaea https://doi.org/10.1594/PANGAEA.837880 https://doi.org/10.1242/jeb.099549 2023-01-20T09:04:17Z Regulating intracellular pH (pHi) is critical for optimising the metabolic activity of corals, yet mechanisms involved in pH regulation and the buffering capacity within coral cells are not well understood. Our study investigated how the presence of symbiotic dinoflagellates affects the response of pHi to pCO2-driven seawater acidification in cells isolated from Pocillopora damicornis. Using the fluorescent dye BCECF-AM, in conjunction with confocal microscopy, we simultaneously characterised the response of pHi in host coral cells and their dinoflagellate symbionts, in symbiotic and non-symbiotic states under saturating light, with and without the photosynthetic inhibitor DCMU. Each treatment was run under control (pH 7.8) and CO2 acidified seawater conditions (decreasing pH from 7.8 - 6.8). After two hours of CO2 addition, by which time the external pH (pHe) had declined to 6.8, the dinoflagellate symbionts had increased their pHi by 0.5 pH units above control levels. In contrast, in both symbiotic and non-symbiotic host coral cells, 15 min of CO2 addition (0.2 pH unit drop in pHe) led to cytoplasmic acidosis equivalent to 0.4 pH units. Despite further seawater acidification over the duration of the experiment, the pHi of non-symbiotic coral cells did not change, though in host cells containing a symbiont cell the pHi recovered to control levels. This recovery was negated when cells were incubated with DCMU. Our results reveal that photosynthetic activity of the endosymbiont is tightly coupled with the ability of the host cell to recover from cellular acidosis after exposure to high CO2 / low pH. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science Pacific |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Acid-base regulation Alkalinity total standard error Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cnidaria Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Figure Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH extracellular intracellular pH change Pocillopora damicornis Replicate Salinity Single species Species Temperature water Time in minutes |
spellingShingle |
Acid-base regulation Alkalinity total standard error Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cnidaria Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Figure Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH extracellular intracellular pH change Pocillopora damicornis Replicate Salinity Single species Species Temperature water Time in minutes Gibbin, Emma M Putnam, H M Davy, Simon K Gates, Ruth D Adaptation of a globally important coccolithophore to ocean warming and acidification |
topic_facet |
Acid-base regulation Alkalinity total standard error Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cnidaria Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Figure Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH extracellular intracellular pH change Pocillopora damicornis Replicate Salinity Single species Species Temperature water Time in minutes |
description |
Regulating intracellular pH (pHi) is critical for optimising the metabolic activity of corals, yet mechanisms involved in pH regulation and the buffering capacity within coral cells are not well understood. Our study investigated how the presence of symbiotic dinoflagellates affects the response of pHi to pCO2-driven seawater acidification in cells isolated from Pocillopora damicornis. Using the fluorescent dye BCECF-AM, in conjunction with confocal microscopy, we simultaneously characterised the response of pHi in host coral cells and their dinoflagellate symbionts, in symbiotic and non-symbiotic states under saturating light, with and without the photosynthetic inhibitor DCMU. Each treatment was run under control (pH 7.8) and CO2 acidified seawater conditions (decreasing pH from 7.8 - 6.8). After two hours of CO2 addition, by which time the external pH (pHe) had declined to 6.8, the dinoflagellate symbionts had increased their pHi by 0.5 pH units above control levels. In contrast, in both symbiotic and non-symbiotic host coral cells, 15 min of CO2 addition (0.2 pH unit drop in pHe) led to cytoplasmic acidosis equivalent to 0.4 pH units. Despite further seawater acidification over the duration of the experiment, the pHi of non-symbiotic coral cells did not change, though in host cells containing a symbiont cell the pHi recovered to control levels. This recovery was negated when cells were incubated with DCMU. Our results reveal that photosynthetic activity of the endosymbiont is tightly coupled with the ability of the host cell to recover from cellular acidosis after exposure to high CO2 / low pH. |
format |
Dataset |
author |
Gibbin, Emma M Putnam, H M Davy, Simon K Gates, Ruth D |
author_facet |
Gibbin, Emma M Putnam, H M Davy, Simon K Gates, Ruth D |
author_sort |
Gibbin, Emma M |
title |
Adaptation of a globally important coccolithophore to ocean warming and acidification |
title_short |
Adaptation of a globally important coccolithophore to ocean warming and acidification |
title_full |
Adaptation of a globally important coccolithophore to ocean warming and acidification |
title_fullStr |
Adaptation of a globally important coccolithophore to ocean warming and acidification |
title_full_unstemmed |
Adaptation of a globally important coccolithophore to ocean warming and acidification |
title_sort |
adaptation of a globally important coccolithophore to ocean warming and acidification |
publisher |
PANGAEA |
publishDate |
2014 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.837880 https://doi.org/10.1594/PANGAEA.837880 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Supplement to: Gibbin, Emma M; Putnam, H M; Davy, Simon K; Gates, Ruth D (2014): Intracellular pH and its response to CO2-driven seawater acidification in symbiotic versus non-symbiotic coral cells. Journal of Experimental Biology, 217(11), 1963-1969, https://doi.org/10.1242/jeb.099549 |
op_relation |
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.837880 https://doi.org/10.1594/PANGAEA.837880 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.837880 https://doi.org/10.1242/jeb.099549 |
_version_ |
1766159502697037824 |