Light-modulated responses of growth and photosynthetic performance to ocean acidification in the model diatom Phaeodactylum tricornutum
Ocean acidification (OA) due to atmospheric CO2 rise is expected to influence marine primary productivity. In order to investigate the interactive effects of OA and light changes on diatoms, we grew Phaeodactylum tricornutum, under ambient (390 ppmv; LC) and elevated CO2 (1000 ppmv; HC) conditions f...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.837678 2023-05-15T17:51:00+02:00 Light-modulated responses of growth and photosynthetic performance to ocean acidification in the model diatom Phaeodactylum tricornutum Li, Yahe Xu, Juntian Gao, Kunshan 2014-10-31 text/tab-separated-values, 3778 data points https://doi.pangaea.de/10.1594/PANGAEA.837678 https://doi.org/10.1594/PANGAEA.837678 en eng PANGAEA Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.837678 https://doi.org/10.1594/PANGAEA.837678 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Li, Yahe; Xu, Juntian; Gao, Kunshan (2014): Light-Modulated Responses of Growth and Photosynthetic Performance to Ocean Acidification in the Model Diatom Phaeodactylum tricornutum. PLoS ONE, 9(5), e96173, https://doi.org/10.1371/journal.pone.0096173 Alkalinity total standard deviation Aragonite saturation state Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved reciprocal of photosynthetic affinity value Carbonate ion Carbonate system computation flag Carbon dioxide Carotenoids Carotenoids per cell Chlorophyll a Chlorophyll a per cell Chlorophyll c Chlorophyll c per cell Chromista Dataset 2014 ftpangaea https://doi.org/10.1594/PANGAEA.837678 https://doi.org/10.1371/journal.pone.0096173 2023-01-20T09:04:14Z Ocean acidification (OA) due to atmospheric CO2 rise is expected to influence marine primary productivity. In order to investigate the interactive effects of OA and light changes on diatoms, we grew Phaeodactylum tricornutum, under ambient (390 ppmv; LC) and elevated CO2 (1000 ppmv; HC) conditions for 80 generations, and measured its physiological performance under different light levels (60 µmol/m**2/s, LL; 200 µmol/m**2/s, ML; 460 µmol/m**2/s, HL) for another 25 generations. The specific growth rate of the HC-grown cells was higher (about 12-18%) than that of the LC-grown ones, with the highest under the ML level. With increasing light levels, the effective photochemical yield of PSII (Fv'/Fm') decreased, but was enhanced by the elevated CO2, especially under the HL level. The cells acclimated to the HC condition showed a higher recovery rate of their photochemical yield of PSII compared to the LC-grown cells. For the HC-grown cells, dissolved inorganic carbon or CO2 levels for half saturation of photosynthesis (K1/2 DIC or K1/2 CO2) increased by 11, 55 and 32%, under the LL, ML and HL levels, reflecting a light dependent down-regulation of carbon concentrating mechanisms (CCMs). The linkage between higher level of the CCMs down-regulation and higher growth rate at ML under OA supports the theory that the saved energy from CCMs down-regulation adds on to enhance the growth of the diatom. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total standard deviation Aragonite saturation state Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved reciprocal of photosynthetic affinity value Carbonate ion Carbonate system computation flag Carbon dioxide Carotenoids Carotenoids per cell Chlorophyll a Chlorophyll a per cell Chlorophyll c Chlorophyll c per cell Chromista |
spellingShingle |
Alkalinity total standard deviation Aragonite saturation state Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved reciprocal of photosynthetic affinity value Carbonate ion Carbonate system computation flag Carbon dioxide Carotenoids Carotenoids per cell Chlorophyll a Chlorophyll a per cell Chlorophyll c Chlorophyll c per cell Chromista Li, Yahe Xu, Juntian Gao, Kunshan Light-modulated responses of growth and photosynthetic performance to ocean acidification in the model diatom Phaeodactylum tricornutum |
topic_facet |
Alkalinity total standard deviation Aragonite saturation state Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved reciprocal of photosynthetic affinity value Carbonate ion Carbonate system computation flag Carbon dioxide Carotenoids Carotenoids per cell Chlorophyll a Chlorophyll a per cell Chlorophyll c Chlorophyll c per cell Chromista |
description |
Ocean acidification (OA) due to atmospheric CO2 rise is expected to influence marine primary productivity. In order to investigate the interactive effects of OA and light changes on diatoms, we grew Phaeodactylum tricornutum, under ambient (390 ppmv; LC) and elevated CO2 (1000 ppmv; HC) conditions for 80 generations, and measured its physiological performance under different light levels (60 µmol/m**2/s, LL; 200 µmol/m**2/s, ML; 460 µmol/m**2/s, HL) for another 25 generations. The specific growth rate of the HC-grown cells was higher (about 12-18%) than that of the LC-grown ones, with the highest under the ML level. With increasing light levels, the effective photochemical yield of PSII (Fv'/Fm') decreased, but was enhanced by the elevated CO2, especially under the HL level. The cells acclimated to the HC condition showed a higher recovery rate of their photochemical yield of PSII compared to the LC-grown cells. For the HC-grown cells, dissolved inorganic carbon or CO2 levels for half saturation of photosynthesis (K1/2 DIC or K1/2 CO2) increased by 11, 55 and 32%, under the LL, ML and HL levels, reflecting a light dependent down-regulation of carbon concentrating mechanisms (CCMs). The linkage between higher level of the CCMs down-regulation and higher growth rate at ML under OA supports the theory that the saved energy from CCMs down-regulation adds on to enhance the growth of the diatom. |
format |
Dataset |
author |
Li, Yahe Xu, Juntian Gao, Kunshan |
author_facet |
Li, Yahe Xu, Juntian Gao, Kunshan |
author_sort |
Li, Yahe |
title |
Light-modulated responses of growth and photosynthetic performance to ocean acidification in the model diatom Phaeodactylum tricornutum |
title_short |
Light-modulated responses of growth and photosynthetic performance to ocean acidification in the model diatom Phaeodactylum tricornutum |
title_full |
Light-modulated responses of growth and photosynthetic performance to ocean acidification in the model diatom Phaeodactylum tricornutum |
title_fullStr |
Light-modulated responses of growth and photosynthetic performance to ocean acidification in the model diatom Phaeodactylum tricornutum |
title_full_unstemmed |
Light-modulated responses of growth and photosynthetic performance to ocean acidification in the model diatom Phaeodactylum tricornutum |
title_sort |
light-modulated responses of growth and photosynthetic performance to ocean acidification in the model diatom phaeodactylum tricornutum |
publisher |
PANGAEA |
publishDate |
2014 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.837678 https://doi.org/10.1594/PANGAEA.837678 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Supplement to: Li, Yahe; Xu, Juntian; Gao, Kunshan (2014): Light-Modulated Responses of Growth and Photosynthetic Performance to Ocean Acidification in the Model Diatom Phaeodactylum tricornutum. PLoS ONE, 9(5), e96173, https://doi.org/10.1371/journal.pone.0096173 |
op_relation |
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.837678 https://doi.org/10.1594/PANGAEA.837678 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.837678 https://doi.org/10.1371/journal.pone.0096173 |
_version_ |
1766157967985475584 |