Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas
Background. Ocean acidification as a result of increased anthropogenic CO2 emissions is occurring in marine and estuarine environments worldwide. The coastal ocean experiences additional daily and seasonal fluctuations in pH that can be lower than projected end of century open ocean pH reductions. P...
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Language: | English |
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PANGAEA
2014
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Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.837671 https://doi.org/10.1594/PANGAEA.837671 |
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.837671 |
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openpolar |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2calc Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Confidence interval Crassostrea gigas Duration number of days Figure Fracture toughness Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Glycogen Group Laboratory experiment Mass Mollusca Mortality Mortality/Survival North Pacific OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) |
spellingShingle |
Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2calc Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Confidence interval Crassostrea gigas Duration number of days Figure Fracture toughness Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Glycogen Group Laboratory experiment Mass Mollusca Mortality Mortality/Survival North Pacific OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Timmins-Schiffman, Emma Coffey, William D Hua, Wilber Nunn, Brook L Dickinson, Gary H Roberts, Steven B Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas |
topic_facet |
Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2calc Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Confidence interval Crassostrea gigas Duration number of days Figure Fracture toughness Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Glycogen Group Laboratory experiment Mass Mollusca Mortality Mortality/Survival North Pacific OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) |
description |
Background. Ocean acidification as a result of increased anthropogenic CO2 emissions is occurring in marine and estuarine environments worldwide. The coastal ocean experiences additional daily and seasonal fluctuations in pH that can be lower than projected end of century open ocean pH reductions. Projected and current ocean acidification have wide-ranging effects on many aquatic organisms, however the exact mechanisms of the impacts of ocean acidification on many of these animals remains to be characterized. Methods. In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different pCO2 levels for four weeks: 400 µatm (pH 8.0), 800 µatm (pH 7.7), 1000 µatm (pH 7.6), or 2800 µatm (pH 7.3). At the end of 4 weeks a variety of physiological parameters were measured to assess the impacts of ocean acidification: tissue glycogen content and fatty acid profile, shell micromechanical properties, and response to acute heat shock. To determine the effects of ocean acidification on the underlying molecular physiology of oysters and their stress response, some of the oysters from 400 µatm and 2800 µatm were exposed to an additional mechanical stress and shotgun proteomics were done on oysters from high and low pCO2 and from with and without mechanical stress. Results. At the end of the four week exposure period, oysters in all four pCO2 environments deposited new shell, but growth rate was not different among the treatments. However, micromechanical properties of the new shell were compromised by elevated pCO2. Elevated pCO2 affected neither whole body fatty acid composition, nor glycogen content, nor mortality rate associated with acute heat shock. Shotgun proteomics revealed that several physiological pathways were significantly affected by ocean acidification, including antioxidant response, carbohydrate metabolism, and transcription and translation. Additionally, the proteomic response to a second stress differed with pCO2, with ... |
format |
Dataset |
author |
Timmins-Schiffman, Emma Coffey, William D Hua, Wilber Nunn, Brook L Dickinson, Gary H Roberts, Steven B |
author_facet |
Timmins-Schiffman, Emma Coffey, William D Hua, Wilber Nunn, Brook L Dickinson, Gary H Roberts, Steven B |
author_sort |
Timmins-Schiffman, Emma |
title |
Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas |
title_short |
Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas |
title_full |
Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas |
title_fullStr |
Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas |
title_full_unstemmed |
Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas |
title_sort |
shotgun proteomics reveals physiological response to ocean acidification in crassostrea gigas |
publisher |
PANGAEA |
publishDate |
2014 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.837671 https://doi.org/10.1594/PANGAEA.837671 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Ocean acidification |
genre_facet |
Crassostrea gigas Ocean acidification |
op_relation |
Timmins-Schiffman, Emma; Coffey, William D; Hua, Wilber; Nunn, Brook L; Dickinson, Gary H; Roberts, Steven B (2014): Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas. PeerJ PrePrints, https://doi.org/10.7287/PEERJ.PREPRINTS.388V1 Timmins-Schiffman, Emma; Coffey, William D; Hua, Wilber; Nunn, Brook L; Dickinson, Gary H; Roberts, Steven B (2014): Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas. BMC Genomics, 15(1), 951, https://doi.org/10.1186/1471-2164-15-951 Roberts, Steven B; Timmins-Schiffman, Emma (2014): Data accompanying "Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas". Figshare, https://doi.org/10.6084/M9.FIGSHARE.1192933 Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.837671 https://doi.org/10.1594/PANGAEA.837671 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.837671 https://doi.org/10.7287/PEERJ.PREPRINTS.388V1 https://doi.org/10.1186/1471-2164-15-951 https://doi.org/10.6084/M9.FIGSHARE.1192933 |
_version_ |
1766393960445509632 |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.837671 2023-05-15T15:58:14+02:00 Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas Timmins-Schiffman, Emma Coffey, William D Hua, Wilber Nunn, Brook L Dickinson, Gary H Roberts, Steven B 2014-10-31 text/tab-separated-values, 23319 data points https://doi.pangaea.de/10.1594/PANGAEA.837671 https://doi.org/10.1594/PANGAEA.837671 en eng PANGAEA Timmins-Schiffman, Emma; Coffey, William D; Hua, Wilber; Nunn, Brook L; Dickinson, Gary H; Roberts, Steven B (2014): Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas. PeerJ PrePrints, https://doi.org/10.7287/PEERJ.PREPRINTS.388V1 Timmins-Schiffman, Emma; Coffey, William D; Hua, Wilber; Nunn, Brook L; Dickinson, Gary H; Roberts, Steven B (2014): Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas. BMC Genomics, 15(1), 951, https://doi.org/10.1186/1471-2164-15-951 Roberts, Steven B; Timmins-Schiffman, Emma (2014): Data accompanying "Shotgun proteomics reveals physiological response to ocean acidification in Crassostrea gigas". Figshare, https://doi.org/10.6084/M9.FIGSHARE.1192933 Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.837671 https://doi.org/10.1594/PANGAEA.837671 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2calc Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Confidence interval Crassostrea gigas Duration number of days Figure Fracture toughness Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Glycogen Group Laboratory experiment Mass Mollusca Mortality Mortality/Survival North Pacific OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Dataset 2014 ftpangaea https://doi.org/10.1594/PANGAEA.837671 https://doi.org/10.7287/PEERJ.PREPRINTS.388V1 https://doi.org/10.1186/1471-2164-15-951 https://doi.org/10.6084/M9.FIGSHARE.1192933 2023-01-20T09:04:14Z Background. Ocean acidification as a result of increased anthropogenic CO2 emissions is occurring in marine and estuarine environments worldwide. The coastal ocean experiences additional daily and seasonal fluctuations in pH that can be lower than projected end of century open ocean pH reductions. Projected and current ocean acidification have wide-ranging effects on many aquatic organisms, however the exact mechanisms of the impacts of ocean acidification on many of these animals remains to be characterized. Methods. In order to assess the impact of ocean acidification on marine invertebrates, Pacific oysters (Crassostrea gigas) were exposed to one of four different pCO2 levels for four weeks: 400 µatm (pH 8.0), 800 µatm (pH 7.7), 1000 µatm (pH 7.6), or 2800 µatm (pH 7.3). At the end of 4 weeks a variety of physiological parameters were measured to assess the impacts of ocean acidification: tissue glycogen content and fatty acid profile, shell micromechanical properties, and response to acute heat shock. To determine the effects of ocean acidification on the underlying molecular physiology of oysters and their stress response, some of the oysters from 400 µatm and 2800 µatm were exposed to an additional mechanical stress and shotgun proteomics were done on oysters from high and low pCO2 and from with and without mechanical stress. Results. At the end of the four week exposure period, oysters in all four pCO2 environments deposited new shell, but growth rate was not different among the treatments. However, micromechanical properties of the new shell were compromised by elevated pCO2. Elevated pCO2 affected neither whole body fatty acid composition, nor glycogen content, nor mortality rate associated with acute heat shock. Shotgun proteomics revealed that several physiological pathways were significantly affected by ocean acidification, including antioxidant response, carbohydrate metabolism, and transcription and translation. Additionally, the proteomic response to a second stress differed with pCO2, with ... Dataset Crassostrea gigas Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science Pacific |