Detection of a variable intracellular acid-labile carbon pool in Thalassiosira weissflogii (Heterokontophyta) and Emiliania huxleyi (Haptophyta) in response to changes in the seawater carbon system
Accumulation of an intracellular pool of carbon (C(i) pool) is one strategy by which marine algae overcome the low abundance of dissolved CO2 (CO2 (aq) ) in modern seawater. To identify the environmental conditions under which algae accumulate an acid-labile C(i) pool, we applied a (14) C pulse-chas...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.836733 2023-05-15T17:52:11+02:00 Detection of a variable intracellular acid-labile carbon pool in Thalassiosira weissflogii (Heterokontophyta) and Emiliania huxleyi (Haptophyta) in response to changes in the seawater carbon system Isensee, Kirsten Erez, Jonathan Stoll, Heather M 2014-10-15 text/tab-separated-values, 7443 data points https://doi.pangaea.de/10.1594/PANGAEA.836733 https://doi.org/10.1594/PANGAEA.836733 en eng PANGAEA Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.836733 https://doi.org/10.1594/PANGAEA.836733 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Isensee, Kirsten; Erez, Jonathan; Stoll, Heather M (2014): Detection of a variable intracellular acid-labile carbon pool in Thalassiosira weissflogii(Heterokontophyta) and Emiliania huxleyi (Haptophyta) in response to changes in the seawater carbon system. Physiologia Plantarum, 150(2), 321-338, https://doi.org/10.1111/ppl.12096 Alkalinity total Aragonite saturation state Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved intracellular pool per cell standard deviation Carbon-14 organic Carbon-14 incorporation per cell Carbonate ion Carbonate system computation flag Carbon dioxide Carbon dioxide/Bicarbonate uptake ratio Carbon incorporation rate per cell Cell biovolume Cell density Chlorophyll a per cell Chromista Emiliania huxleyi Figure Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Haptophyta Laboratory experiment Laboratory strains Not applicable OA-ICC Ocean Acidification International Coordination Centre Ochrophyta Other metabolic rates Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Phytoplankton Dataset 2014 ftpangaea https://doi.org/10.1594/PANGAEA.836733 https://doi.org/10.1111/ppl.12096 2023-01-20T09:03:59Z Accumulation of an intracellular pool of carbon (C(i) pool) is one strategy by which marine algae overcome the low abundance of dissolved CO2 (CO2 (aq) ) in modern seawater. To identify the environmental conditions under which algae accumulate an acid-labile C(i) pool, we applied a (14) C pulse-chase method, used originally in dinoflagellates, to two new classes of algae, coccolithophorids and diatoms. This method measures the carbon accumulation inside the cells without altering the medium carbon chemistry or culture cell density. We found that the diatom Thalassiosira weissflogii [(Grunow) G. Fryxell & Hasle] and a calcifying strain of the coccolithophorid Emiliania huxleyi [(Lohmann) W. W. Hay & H. P. Mohler] develop significant acid-labile C(i) pools. C(i) pools are measureable in cells cultured in media with 2-30 µmol/l CO2 (aq), corresponding to a medium pH of 8.6-7.9. The absolute C(i) pool was greater for the larger celled diatoms. For both algal classes, the C(i) pool became a negligible contributor to photosynthesis once CO2 (aq) exceeded 30 µmol/l. Combining the (14) C pulse-chase method and (14) C disequilibrium method enabled us to assess whether E. huxleyi and T. weissflogii exhibited thresholds for foregoing accumulation of DIC or reduced the reliance on bicarbonate uptake with increasing CO2 (aq) . We showed that the C(i) pool decreases with higher CO2 :HCO3 (-) uptake rates. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science Fryxell ENVELOPE(163.183,163.183,-77.617,-77.617) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total Aragonite saturation state Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved intracellular pool per cell standard deviation Carbon-14 organic Carbon-14 incorporation per cell Carbonate ion Carbonate system computation flag Carbon dioxide Carbon dioxide/Bicarbonate uptake ratio Carbon incorporation rate per cell Cell biovolume Cell density Chlorophyll a per cell Chromista Emiliania huxleyi Figure Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Haptophyta Laboratory experiment Laboratory strains Not applicable OA-ICC Ocean Acidification International Coordination Centre Ochrophyta Other metabolic rates Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Phytoplankton |
spellingShingle |
Alkalinity total Aragonite saturation state Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved intracellular pool per cell standard deviation Carbon-14 organic Carbon-14 incorporation per cell Carbonate ion Carbonate system computation flag Carbon dioxide Carbon dioxide/Bicarbonate uptake ratio Carbon incorporation rate per cell Cell biovolume Cell density Chlorophyll a per cell Chromista Emiliania huxleyi Figure Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Haptophyta Laboratory experiment Laboratory strains Not applicable OA-ICC Ocean Acidification International Coordination Centre Ochrophyta Other metabolic rates Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Phytoplankton Isensee, Kirsten Erez, Jonathan Stoll, Heather M Detection of a variable intracellular acid-labile carbon pool in Thalassiosira weissflogii (Heterokontophyta) and Emiliania huxleyi (Haptophyta) in response to changes in the seawater carbon system |
topic_facet |
Alkalinity total Aragonite saturation state Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved intracellular pool per cell standard deviation Carbon-14 organic Carbon-14 incorporation per cell Carbonate ion Carbonate system computation flag Carbon dioxide Carbon dioxide/Bicarbonate uptake ratio Carbon incorporation rate per cell Cell biovolume Cell density Chlorophyll a per cell Chromista Emiliania huxleyi Figure Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Haptophyta Laboratory experiment Laboratory strains Not applicable OA-ICC Ocean Acidification International Coordination Centre Ochrophyta Other metabolic rates Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Phytoplankton |
description |
Accumulation of an intracellular pool of carbon (C(i) pool) is one strategy by which marine algae overcome the low abundance of dissolved CO2 (CO2 (aq) ) in modern seawater. To identify the environmental conditions under which algae accumulate an acid-labile C(i) pool, we applied a (14) C pulse-chase method, used originally in dinoflagellates, to two new classes of algae, coccolithophorids and diatoms. This method measures the carbon accumulation inside the cells without altering the medium carbon chemistry or culture cell density. We found that the diatom Thalassiosira weissflogii [(Grunow) G. Fryxell & Hasle] and a calcifying strain of the coccolithophorid Emiliania huxleyi [(Lohmann) W. W. Hay & H. P. Mohler] develop significant acid-labile C(i) pools. C(i) pools are measureable in cells cultured in media with 2-30 µmol/l CO2 (aq), corresponding to a medium pH of 8.6-7.9. The absolute C(i) pool was greater for the larger celled diatoms. For both algal classes, the C(i) pool became a negligible contributor to photosynthesis once CO2 (aq) exceeded 30 µmol/l. Combining the (14) C pulse-chase method and (14) C disequilibrium method enabled us to assess whether E. huxleyi and T. weissflogii exhibited thresholds for foregoing accumulation of DIC or reduced the reliance on bicarbonate uptake with increasing CO2 (aq) . We showed that the C(i) pool decreases with higher CO2 :HCO3 (-) uptake rates. |
format |
Dataset |
author |
Isensee, Kirsten Erez, Jonathan Stoll, Heather M |
author_facet |
Isensee, Kirsten Erez, Jonathan Stoll, Heather M |
author_sort |
Isensee, Kirsten |
title |
Detection of a variable intracellular acid-labile carbon pool in Thalassiosira weissflogii (Heterokontophyta) and Emiliania huxleyi (Haptophyta) in response to changes in the seawater carbon system |
title_short |
Detection of a variable intracellular acid-labile carbon pool in Thalassiosira weissflogii (Heterokontophyta) and Emiliania huxleyi (Haptophyta) in response to changes in the seawater carbon system |
title_full |
Detection of a variable intracellular acid-labile carbon pool in Thalassiosira weissflogii (Heterokontophyta) and Emiliania huxleyi (Haptophyta) in response to changes in the seawater carbon system |
title_fullStr |
Detection of a variable intracellular acid-labile carbon pool in Thalassiosira weissflogii (Heterokontophyta) and Emiliania huxleyi (Haptophyta) in response to changes in the seawater carbon system |
title_full_unstemmed |
Detection of a variable intracellular acid-labile carbon pool in Thalassiosira weissflogii (Heterokontophyta) and Emiliania huxleyi (Haptophyta) in response to changes in the seawater carbon system |
title_sort |
detection of a variable intracellular acid-labile carbon pool in thalassiosira weissflogii (heterokontophyta) and emiliania huxleyi (haptophyta) in response to changes in the seawater carbon system |
publisher |
PANGAEA |
publishDate |
2014 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.836733 https://doi.org/10.1594/PANGAEA.836733 |
long_lat |
ENVELOPE(163.183,163.183,-77.617,-77.617) |
geographic |
Fryxell |
geographic_facet |
Fryxell |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Supplement to: Isensee, Kirsten; Erez, Jonathan; Stoll, Heather M (2014): Detection of a variable intracellular acid-labile carbon pool in Thalassiosira weissflogii(Heterokontophyta) and Emiliania huxleyi (Haptophyta) in response to changes in the seawater carbon system. Physiologia Plantarum, 150(2), 321-338, https://doi.org/10.1111/ppl.12096 |
op_relation |
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.836733 https://doi.org/10.1594/PANGAEA.836733 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.836733 https://doi.org/10.1111/ppl.12096 |
_version_ |
1766159563650760704 |