Effects of increasing seawater carbon dioxide concentrations on chain formation of the diatom Asterionellopsis glacialis
Diatoms can occur as single cells or as chain-forming aggregates. These two strategies affect buoyancy, predator evasion, light absorption and nutrient uptake. Adjacent cells in chains establish connections through various processes that determine strength and flexibility of the bonds, and at distin...
Main Authors: | , , , , |
---|---|
Format: | Dataset |
Language: | English |
Published: |
PANGAEA
2014
|
Subjects: | |
Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.836367 https://doi.org/10.1594/PANGAEA.836367 |
id |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.836367 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total Aragonite saturation state Asterionellopsis glacialis Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved organic dissolved exudation per cell particulate production per cell Carbon/Nitrogen ratio Carbon/Phosphorus ratio Carbonate ion Carbonate system computation flag Carbon dioxide Chromista Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate Laboratory experiment Nitrogen/Phosphorus ratio North Atlantic OA-ICC Ocean Acidification International Coordination Centre Ochrophyta Open ocean Other metabolic rates Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Particulate organic carbon Particulate organic nitrogen per cell Particulate organic phosphorus per cell Pelagos Percentage pH Phosphorus |
spellingShingle |
Alkalinity total Aragonite saturation state Asterionellopsis glacialis Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved organic dissolved exudation per cell particulate production per cell Carbon/Nitrogen ratio Carbon/Phosphorus ratio Carbonate ion Carbonate system computation flag Carbon dioxide Chromista Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate Laboratory experiment Nitrogen/Phosphorus ratio North Atlantic OA-ICC Ocean Acidification International Coordination Centre Ochrophyta Open ocean Other metabolic rates Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Particulate organic carbon Particulate organic nitrogen per cell Particulate organic phosphorus per cell Pelagos Percentage pH Phosphorus Barcelos e Ramos, Joana Schulz, Kai Georg Brownlee, Colin Sett, Scarlett Azevedo, Eduardo Brito Effects of increasing seawater carbon dioxide concentrations on chain formation of the diatom Asterionellopsis glacialis |
topic_facet |
Alkalinity total Aragonite saturation state Asterionellopsis glacialis Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved organic dissolved exudation per cell particulate production per cell Carbon/Nitrogen ratio Carbon/Phosphorus ratio Carbonate ion Carbonate system computation flag Carbon dioxide Chromista Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate Laboratory experiment Nitrogen/Phosphorus ratio North Atlantic OA-ICC Ocean Acidification International Coordination Centre Ochrophyta Open ocean Other metabolic rates Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Particulate organic carbon Particulate organic nitrogen per cell Particulate organic phosphorus per cell Pelagos Percentage pH Phosphorus |
description |
Diatoms can occur as single cells or as chain-forming aggregates. These two strategies affect buoyancy, predator evasion, light absorption and nutrient uptake. Adjacent cells in chains establish connections through various processes that determine strength and flexibility of the bonds, and at distinct cellular locations defining colony structure. Chain length has been found to vary with temperature and nutrient availability as well as being positively correlated with growth rate. However, the potential effect of enhanced carbon dioxide (CO2) concentrations and consequent changes in seawater carbonate chemistry on chain formation is virtually unknown. Here we report on experiments with semi-continuous cultures of the freshly isolated diatom Asterionellopsis glacialis grown under increasing CO2 levels ranging from 320 to 3400 µatm. We show that the number of cells comprising a chain, and therefore chain length, increases with rising CO2 concentrations. We also demonstrate that while cell division rate changes with CO2 concentrations, carbon, nitrogen and phosphorus cellular quotas vary proportionally, evident by unchanged organic matter ratios. Finally, beyond the optimum CO2 concentration for growth, carbon allocation changes from cellular storage to increased exudation of dissolved organic carbon. The observed structural adjustment in colony size could enable growth at high CO2 levels, since longer, spiral-shaped chains are likely to create microclimates with higher pH during the light period. Moreover increased chain length of Asterionellopsis glacialis may influence buoyancy and, consequently, affect competitive fitness as well as sinking rates. This would potentially impact the delicate balance between the microbial loop and export of organic matter, with consequences for atmospheric carbon dioxide. |
format |
Dataset |
author |
Barcelos e Ramos, Joana Schulz, Kai Georg Brownlee, Colin Sett, Scarlett Azevedo, Eduardo Brito |
author_facet |
Barcelos e Ramos, Joana Schulz, Kai Georg Brownlee, Colin Sett, Scarlett Azevedo, Eduardo Brito |
author_sort |
Barcelos e Ramos, Joana |
title |
Effects of increasing seawater carbon dioxide concentrations on chain formation of the diatom Asterionellopsis glacialis |
title_short |
Effects of increasing seawater carbon dioxide concentrations on chain formation of the diatom Asterionellopsis glacialis |
title_full |
Effects of increasing seawater carbon dioxide concentrations on chain formation of the diatom Asterionellopsis glacialis |
title_fullStr |
Effects of increasing seawater carbon dioxide concentrations on chain formation of the diatom Asterionellopsis glacialis |
title_full_unstemmed |
Effects of increasing seawater carbon dioxide concentrations on chain formation of the diatom Asterionellopsis glacialis |
title_sort |
effects of increasing seawater carbon dioxide concentrations on chain formation of the diatom asterionellopsis glacialis |
publisher |
PANGAEA |
publishDate |
2014 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.836367 https://doi.org/10.1594/PANGAEA.836367 |
genre |
North Atlantic Ocean acidification |
genre_facet |
North Atlantic Ocean acidification |
op_source |
Supplement to: Barcelos e Ramos, Joana; Schulz, Kai Georg; Brownlee, Colin; Sett, Scarlett; Azevedo, Eduardo Brito (2014): Effects of Increasing Seawater Carbon Dioxide Concentrations on Chain Formation of the Diatom Asterionellopsis glacialis. PLoS ONE, 9(3), e90749, https://doi.org/10.1371/journal.pone.0090749 |
op_relation |
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0 [webpage]. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.836367 https://doi.org/10.1594/PANGAEA.836367 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.83636710.1371/journal.pone.0090749 |
_version_ |
1810464831805849600 |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.836367 2024-09-15T18:24:28+00:00 Effects of increasing seawater carbon dioxide concentrations on chain formation of the diatom Asterionellopsis glacialis Barcelos e Ramos, Joana Schulz, Kai Georg Brownlee, Colin Sett, Scarlett Azevedo, Eduardo Brito 2014 text/tab-separated-values, 616 data points https://doi.pangaea.de/10.1594/PANGAEA.836367 https://doi.org/10.1594/PANGAEA.836367 en eng PANGAEA Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0 [webpage]. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.836367 https://doi.org/10.1594/PANGAEA.836367 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Barcelos e Ramos, Joana; Schulz, Kai Georg; Brownlee, Colin; Sett, Scarlett; Azevedo, Eduardo Brito (2014): Effects of Increasing Seawater Carbon Dioxide Concentrations on Chain Formation of the Diatom Asterionellopsis glacialis. PLoS ONE, 9(3), e90749, https://doi.org/10.1371/journal.pone.0090749 Alkalinity total Aragonite saturation state Asterionellopsis glacialis Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved organic dissolved exudation per cell particulate production per cell Carbon/Nitrogen ratio Carbon/Phosphorus ratio Carbonate ion Carbonate system computation flag Carbon dioxide Chromista Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate Laboratory experiment Nitrogen/Phosphorus ratio North Atlantic OA-ICC Ocean Acidification International Coordination Centre Ochrophyta Open ocean Other metabolic rates Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Particulate organic carbon Particulate organic nitrogen per cell Particulate organic phosphorus per cell Pelagos Percentage pH Phosphorus dataset 2014 ftpangaea https://doi.org/10.1594/PANGAEA.83636710.1371/journal.pone.0090749 2024-07-24T02:31:32Z Diatoms can occur as single cells or as chain-forming aggregates. These two strategies affect buoyancy, predator evasion, light absorption and nutrient uptake. Adjacent cells in chains establish connections through various processes that determine strength and flexibility of the bonds, and at distinct cellular locations defining colony structure. Chain length has been found to vary with temperature and nutrient availability as well as being positively correlated with growth rate. However, the potential effect of enhanced carbon dioxide (CO2) concentrations and consequent changes in seawater carbonate chemistry on chain formation is virtually unknown. Here we report on experiments with semi-continuous cultures of the freshly isolated diatom Asterionellopsis glacialis grown under increasing CO2 levels ranging from 320 to 3400 µatm. We show that the number of cells comprising a chain, and therefore chain length, increases with rising CO2 concentrations. We also demonstrate that while cell division rate changes with CO2 concentrations, carbon, nitrogen and phosphorus cellular quotas vary proportionally, evident by unchanged organic matter ratios. Finally, beyond the optimum CO2 concentration for growth, carbon allocation changes from cellular storage to increased exudation of dissolved organic carbon. The observed structural adjustment in colony size could enable growth at high CO2 levels, since longer, spiral-shaped chains are likely to create microclimates with higher pH during the light period. Moreover increased chain length of Asterionellopsis glacialis may influence buoyancy and, consequently, affect competitive fitness as well as sinking rates. This would potentially impact the delicate balance between the microbial loop and export of organic matter, with consequences for atmospheric carbon dioxide. Dataset North Atlantic Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |