Photophysiological responses of Southern Ocean phytoplankton to changes in CO2 concentrations: Short-term versus acclimation effects
The present study examines how different pCO2 acclimations affect the CO2- and light-dependence of photophysiological processes and O2 fluxes in four Southern Ocean (SO) key phytoplankton species. We grew Chaetoceros debilis (Cleve), Pseudo-nitzschia subcurvata (Hasle), Fragilariopsis kerguelensis (...
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Language: | English |
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PANGAEA
2014
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Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.833713 https://doi.org/10.1594/PANGAEA.833713 |
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.833713 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total standard deviation Antarctic Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chaetoceros debilis Chromista Connectivity between photosystem II Effective absorbance cross-section of photosystem II Effective quantum yield Electron transport rate relative Figure Fragilariopsis kerguelensis Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gross oxygen evolution per chlorophyll a Haptophyta Irradiance Laboratory experiment Laboratory strains Net oxygen evolution Non photochemical quenching |
spellingShingle |
Alkalinity total standard deviation Antarctic Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chaetoceros debilis Chromista Connectivity between photosystem II Effective absorbance cross-section of photosystem II Effective quantum yield Electron transport rate relative Figure Fragilariopsis kerguelensis Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gross oxygen evolution per chlorophyll a Haptophyta Irradiance Laboratory experiment Laboratory strains Net oxygen evolution Non photochemical quenching Trimborn, Scarlett Thoms, Silke Petrou, Katherina Kranz, Sven A Rost, Björn Photophysiological responses of Southern Ocean phytoplankton to changes in CO2 concentrations: Short-term versus acclimation effects |
topic_facet |
Alkalinity total standard deviation Antarctic Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chaetoceros debilis Chromista Connectivity between photosystem II Effective absorbance cross-section of photosystem II Effective quantum yield Electron transport rate relative Figure Fragilariopsis kerguelensis Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gross oxygen evolution per chlorophyll a Haptophyta Irradiance Laboratory experiment Laboratory strains Net oxygen evolution Non photochemical quenching |
description |
The present study examines how different pCO2 acclimations affect the CO2- and light-dependence of photophysiological processes and O2 fluxes in four Southern Ocean (SO) key phytoplankton species. We grew Chaetoceros debilis (Cleve), Pseudo-nitzschia subcurvata (Hasle), Fragilariopsis kerguelensis (O'Meara) and Phaeocystis antarctica (Karsten) under low (160 µatm) and high (1000 ?atm) pCO2. The CO2- and light-dependence of fluorescence parameters of photosystem II (PSII) were determined by means of a fluorescence induction relaxation system (FIRe). In all tested species, nonphotochemical quenching (NPQ) is the primary photoprotection strategy in response to short-term exposure to high light or low CO2 concentrations. In C. debilis and P. subcurvata, PSII connectivity (p) and functional absorption cross-sections of PSII in ambient light (sigma PSII') also contributed to photoprotection while changes in re-oxidation times of Qa acceptor (tQa) were more significant in F. kerguelensis. The latter was also the only species being responsive to high acclimation pCO2, as these cells had enhanced relative electron transport rates (rETRs) and sigma PSII' while tQa and p were reduced under short-term exposure to high irradiance. Low CO2-acclimated cells of F. kerguelensis and all pCO2 acclimations of C. debilis and P. subcurvata showed dynamic photoinhibition with increasing irradiance. To test for the role and presence of the Mehler reaction in C. debilis and P. subcurvata, the light-dependence of O2 fluxes was estimated using membrane inlet mass spectrometry (MIMS). Our results show that the Mehler reaction is absent in both species under the tested conditions. We also observed that dark respiration was strongly reduced under high pCO2 in C. debilis while it remained unaltered in P. subcurvata. Our study revealed species-specific differences in the photophysiological responses to pCO2, both on the acclimation as well as the short-term level. |
format |
Dataset |
author |
Trimborn, Scarlett Thoms, Silke Petrou, Katherina Kranz, Sven A Rost, Björn |
author_facet |
Trimborn, Scarlett Thoms, Silke Petrou, Katherina Kranz, Sven A Rost, Björn |
author_sort |
Trimborn, Scarlett |
title |
Photophysiological responses of Southern Ocean phytoplankton to changes in CO2 concentrations: Short-term versus acclimation effects |
title_short |
Photophysiological responses of Southern Ocean phytoplankton to changes in CO2 concentrations: Short-term versus acclimation effects |
title_full |
Photophysiological responses of Southern Ocean phytoplankton to changes in CO2 concentrations: Short-term versus acclimation effects |
title_fullStr |
Photophysiological responses of Southern Ocean phytoplankton to changes in CO2 concentrations: Short-term versus acclimation effects |
title_full_unstemmed |
Photophysiological responses of Southern Ocean phytoplankton to changes in CO2 concentrations: Short-term versus acclimation effects |
title_sort |
photophysiological responses of southern ocean phytoplankton to changes in co2 concentrations: short-term versus acclimation effects |
publisher |
PANGAEA |
publishDate |
2014 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.833713 https://doi.org/10.1594/PANGAEA.833713 |
genre |
Antarc* Antarctic Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica Southern Ocean |
op_source |
Supplement to: Trimborn, Scarlett; Thoms, Silke; Petrou, Katherina; Kranz, Sven A; Rost, Björn (2014): Photophysiological responses of Southern Ocean phytoplankton to changes in CO2 concentrations: Short-term versus acclimation effects. Journal of Experimental Marine Biology and Ecology, 451, 44-54, https://doi.org/10.1016/j.jembe.2013.11.001 |
op_relation |
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0 [webpage]. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.833713 https://doi.org/10.1594/PANGAEA.833713 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.83371310.1016/j.jembe.2013.11.001 |
_version_ |
1810497546619977728 |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.833713 2024-09-15T17:47:52+00:00 Photophysiological responses of Southern Ocean phytoplankton to changes in CO2 concentrations: Short-term versus acclimation effects Trimborn, Scarlett Thoms, Silke Petrou, Katherina Kranz, Sven A Rost, Björn 2014 text/tab-separated-values, 36257 data points https://doi.pangaea.de/10.1594/PANGAEA.833713 https://doi.org/10.1594/PANGAEA.833713 en eng PANGAEA Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0 [webpage]. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.833713 https://doi.org/10.1594/PANGAEA.833713 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Trimborn, Scarlett; Thoms, Silke; Petrou, Katherina; Kranz, Sven A; Rost, Björn (2014): Photophysiological responses of Southern Ocean phytoplankton to changes in CO2 concentrations: Short-term versus acclimation effects. Journal of Experimental Marine Biology and Ecology, 451, 44-54, https://doi.org/10.1016/j.jembe.2013.11.001 Alkalinity total standard deviation Antarctic Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chaetoceros debilis Chromista Connectivity between photosystem II Effective absorbance cross-section of photosystem II Effective quantum yield Electron transport rate relative Figure Fragilariopsis kerguelensis Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gross oxygen evolution per chlorophyll a Haptophyta Irradiance Laboratory experiment Laboratory strains Net oxygen evolution Non photochemical quenching dataset 2014 ftpangaea https://doi.org/10.1594/PANGAEA.83371310.1016/j.jembe.2013.11.001 2024-07-24T02:31:32Z The present study examines how different pCO2 acclimations affect the CO2- and light-dependence of photophysiological processes and O2 fluxes in four Southern Ocean (SO) key phytoplankton species. We grew Chaetoceros debilis (Cleve), Pseudo-nitzschia subcurvata (Hasle), Fragilariopsis kerguelensis (O'Meara) and Phaeocystis antarctica (Karsten) under low (160 µatm) and high (1000 ?atm) pCO2. The CO2- and light-dependence of fluorescence parameters of photosystem II (PSII) were determined by means of a fluorescence induction relaxation system (FIRe). In all tested species, nonphotochemical quenching (NPQ) is the primary photoprotection strategy in response to short-term exposure to high light or low CO2 concentrations. In C. debilis and P. subcurvata, PSII connectivity (p) and functional absorption cross-sections of PSII in ambient light (sigma PSII') also contributed to photoprotection while changes in re-oxidation times of Qa acceptor (tQa) were more significant in F. kerguelensis. The latter was also the only species being responsive to high acclimation pCO2, as these cells had enhanced relative electron transport rates (rETRs) and sigma PSII' while tQa and p were reduced under short-term exposure to high irradiance. Low CO2-acclimated cells of F. kerguelensis and all pCO2 acclimations of C. debilis and P. subcurvata showed dynamic photoinhibition with increasing irradiance. To test for the role and presence of the Mehler reaction in C. debilis and P. subcurvata, the light-dependence of O2 fluxes was estimated using membrane inlet mass spectrometry (MIMS). Our results show that the Mehler reaction is absent in both species under the tested conditions. We also observed that dark respiration was strongly reduced under high pCO2 in C. debilis while it remained unaltered in P. subcurvata. Our study revealed species-specific differences in the photophysiological responses to pCO2, both on the acclimation as well as the short-term level. Dataset Antarc* Antarctic Antarctica Southern Ocean PANGAEA - Data Publisher for Earth & Environmental Science |