Gene expression profiling in gills of the great spider crab Hyas araneus in respond to ocean acidification and warming, supplementary data
Hypercapnia and elevated temperatures resulting from climate change may have adverse consequences for many marine organisms. While diverse physiological and ecological effects have been identified, changes in those molecular mechanisms, which shape the physiological phenotype of a species and limit...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.833705 2024-09-15T18:27:43+00:00 Gene expression profiling in gills of the great spider crab Hyas araneus in respond to ocean acidification and warming, supplementary data Harms, Lars Frickenhaus, Stephan Schiffer, Melanie Mark, Felix Christopher Storch, Daniela Held, Christoph Lucassen, Magnus 2014 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet, 115.3 kBytes https://doi.pangaea.de/10.1594/PANGAEA.833705 https://doi.org/10.1594/PANGAEA.833705 en eng PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.833705 https://doi.org/10.1594/PANGAEA.833705 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Harms, Lars; Frickenhaus, Stephan; Schiffer, Melanie; Mark, Felix Christopher; Storch, Daniela; Held, Christoph; Pörtner, Hans-Otto; Lucassen, Magnus (2014): Gene expression profiling in gills of the great spider crab Hyas araneus in response to ocean acidification and warming. BMC Genomics, BMC Genomics, 15(1), 789, https://doi.org/10.1186/1471-2164-15-789 BIOACID Biological Impacts of Ocean Acidification dataset 2014 ftpangaea https://doi.org/10.1594/PANGAEA.83370510.1186/1471-2164-15-789 2024-07-24T02:31:32Z Hypercapnia and elevated temperatures resulting from climate change may have adverse consequences for many marine organisms. While diverse physiological and ecological effects have been identified, changes in those molecular mechanisms, which shape the physiological phenotype of a species and limit its capacity to compensate, remain poorly understood. Here, we use global gene expression profiling through RNA-Sequencing to study the transcriptional responses to ocean acidification and warming in gills of the boreal spider crab Hyas araneus exposed medium-term (10 weeks) to intermediate (1,120 µatm) and high (1,960 µatm) PCO2 at different temperatures (5°C and 10°C). The analyses reveal shifts in steady state gene expression from control to intermediate and from intermediate to high CO2 exposures. At 5°C acid-base, energy metabolism and stress response related genes were upregulated at intermediate PCO2, whereas high PCO2 induced a relative reduction in expression to levels closer to controls. A similar pattern was found at elevated temperature (10°C). There was a strong coordination between acid-base, metabolic and stress-related processes. Hemolymph parameters at intermediate PCO2 indicate enhanced capacity in acid-base compensation potentially supported by upregulation of a V-ATPase. The likely enhanced energy demand might be met by the upregulation of the electron transport system (ETS), but may lead to increased oxidative stress reflected in upregulated antioxidant defense transcripts. These mechanisms were attenuated by high PCO2, possibly as a result of limited acid-base compensation and metabolic down-regulation. Our findings indicate a PCO2 dependent threshold beyond which compensation by acclimation fails progressively. They also indicate a limited ability of this stenoecious crustacean to compensate for the effects of ocean acidification with and without concomitant warming. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
BIOACID Biological Impacts of Ocean Acidification |
spellingShingle |
BIOACID Biological Impacts of Ocean Acidification Harms, Lars Frickenhaus, Stephan Schiffer, Melanie Mark, Felix Christopher Storch, Daniela Held, Christoph Lucassen, Magnus Gene expression profiling in gills of the great spider crab Hyas araneus in respond to ocean acidification and warming, supplementary data |
topic_facet |
BIOACID Biological Impacts of Ocean Acidification |
description |
Hypercapnia and elevated temperatures resulting from climate change may have adverse consequences for many marine organisms. While diverse physiological and ecological effects have been identified, changes in those molecular mechanisms, which shape the physiological phenotype of a species and limit its capacity to compensate, remain poorly understood. Here, we use global gene expression profiling through RNA-Sequencing to study the transcriptional responses to ocean acidification and warming in gills of the boreal spider crab Hyas araneus exposed medium-term (10 weeks) to intermediate (1,120 µatm) and high (1,960 µatm) PCO2 at different temperatures (5°C and 10°C). The analyses reveal shifts in steady state gene expression from control to intermediate and from intermediate to high CO2 exposures. At 5°C acid-base, energy metabolism and stress response related genes were upregulated at intermediate PCO2, whereas high PCO2 induced a relative reduction in expression to levels closer to controls. A similar pattern was found at elevated temperature (10°C). There was a strong coordination between acid-base, metabolic and stress-related processes. Hemolymph parameters at intermediate PCO2 indicate enhanced capacity in acid-base compensation potentially supported by upregulation of a V-ATPase. The likely enhanced energy demand might be met by the upregulation of the electron transport system (ETS), but may lead to increased oxidative stress reflected in upregulated antioxidant defense transcripts. These mechanisms were attenuated by high PCO2, possibly as a result of limited acid-base compensation and metabolic down-regulation. Our findings indicate a PCO2 dependent threshold beyond which compensation by acclimation fails progressively. They also indicate a limited ability of this stenoecious crustacean to compensate for the effects of ocean acidification with and without concomitant warming. |
format |
Dataset |
author |
Harms, Lars Frickenhaus, Stephan Schiffer, Melanie Mark, Felix Christopher Storch, Daniela Held, Christoph Lucassen, Magnus |
author_facet |
Harms, Lars Frickenhaus, Stephan Schiffer, Melanie Mark, Felix Christopher Storch, Daniela Held, Christoph Lucassen, Magnus |
author_sort |
Harms, Lars |
title |
Gene expression profiling in gills of the great spider crab Hyas araneus in respond to ocean acidification and warming, supplementary data |
title_short |
Gene expression profiling in gills of the great spider crab Hyas araneus in respond to ocean acidification and warming, supplementary data |
title_full |
Gene expression profiling in gills of the great spider crab Hyas araneus in respond to ocean acidification and warming, supplementary data |
title_fullStr |
Gene expression profiling in gills of the great spider crab Hyas araneus in respond to ocean acidification and warming, supplementary data |
title_full_unstemmed |
Gene expression profiling in gills of the great spider crab Hyas araneus in respond to ocean acidification and warming, supplementary data |
title_sort |
gene expression profiling in gills of the great spider crab hyas araneus in respond to ocean acidification and warming, supplementary data |
publisher |
PANGAEA |
publishDate |
2014 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.833705 https://doi.org/10.1594/PANGAEA.833705 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Supplement to: Harms, Lars; Frickenhaus, Stephan; Schiffer, Melanie; Mark, Felix Christopher; Storch, Daniela; Held, Christoph; Pörtner, Hans-Otto; Lucassen, Magnus (2014): Gene expression profiling in gills of the great spider crab Hyas araneus in response to ocean acidification and warming. BMC Genomics, BMC Genomics, 15(1), 789, https://doi.org/10.1186/1471-2164-15-789 |
op_relation |
https://doi.pangaea.de/10.1594/PANGAEA.833705 https://doi.org/10.1594/PANGAEA.833705 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.83370510.1186/1471-2164-15-789 |
_version_ |
1810468969151201280 |