Expression of biomineralization-related ion transport genes in Emiliania huxleyi
Biomineralization in the marine phytoplankton Emiliania huxleyi is a stringently controlled intracellular process. The molecular basis of coccolith production is still relatively unknown although its importance in global biogeochemical cycles and varying sensitivity to increased pCO2 levels has been...
Main Authors: | , , , , , |
---|---|
Format: | Dataset |
Language: | English |
Published: |
PANGAEA
2011
|
Subjects: | |
Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.832450 https://doi.org/10.1594/PANGAEA.832450 |
id |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.832450 |
---|---|
record_format |
openpolar |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.832450 2023-05-15T17:51:18+02:00 Expression of biomineralization-related ion transport genes in Emiliania huxleyi Mackinder, Luke C M Wheeler, Glen Schroeder, Declan C von Dassow, Peter Riebesell, Ulf Brownlee, Colin 2011-05-12 application/zip, 3 datasets https://doi.pangaea.de/10.1594/PANGAEA.832450 https://doi.org/10.1594/PANGAEA.832450 en eng PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.832450 https://doi.org/10.1594/PANGAEA.832450 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Mackinder, Luke C M; Wheeler, Glen; Schroeder, Declan C; von Dassow, Peter; Riebesell, Ulf; Brownlee, Colin (2011): Expression of biomineralization-related ion transport genes in Emiliania huxleyi. Environmental Microbiology, 13(12), 3250-3265, https://doi.org/10.1111/j.1462-2920.2011.02561.x BIOACID Biological Impacts of Ocean Acidification Dataset 2011 ftpangaea https://doi.org/10.1594/PANGAEA.832450 https://doi.org/10.1111/j.1462-2920.2011.02561.x 2023-01-20T07:33:17Z Biomineralization in the marine phytoplankton Emiliania huxleyi is a stringently controlled intracellular process. The molecular basis of coccolith production is still relatively unknown although its importance in global biogeochemical cycles and varying sensitivity to increased pCO2 levels has been well documented. This study looks into the role of several candidate Ca2+, H+ and inorganic carbon transport genes in E. huxleyi, using quantitative reverse transcriptase PCR. Differential gene expression analysis was investigated in two isogenic pairs of calcifying and non-calcifying strains of E. huxleyi and cultures grown at various Ca2+ concentrations to alter calcite production. We show that calcification correlated to the consistent upregulation of a putative HCO3- transporter belonging to the solute carrier 4 (SLC4) family, a Ca2+/H+ exchanger belonging to the CAX family of exchangers and a vacuolar H+-ATPase. We also show that the coccolith-associated protein, GPA is downregulated in calcifying cells. The data provide strong evidence that these genes play key roles in E. huxleyi biomineralization. Based on the gene expression data and the current literature a working model for biomineralization-related ion transport in coccolithophores is presented. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
BIOACID Biological Impacts of Ocean Acidification |
spellingShingle |
BIOACID Biological Impacts of Ocean Acidification Mackinder, Luke C M Wheeler, Glen Schroeder, Declan C von Dassow, Peter Riebesell, Ulf Brownlee, Colin Expression of biomineralization-related ion transport genes in Emiliania huxleyi |
topic_facet |
BIOACID Biological Impacts of Ocean Acidification |
description |
Biomineralization in the marine phytoplankton Emiliania huxleyi is a stringently controlled intracellular process. The molecular basis of coccolith production is still relatively unknown although its importance in global biogeochemical cycles and varying sensitivity to increased pCO2 levels has been well documented. This study looks into the role of several candidate Ca2+, H+ and inorganic carbon transport genes in E. huxleyi, using quantitative reverse transcriptase PCR. Differential gene expression analysis was investigated in two isogenic pairs of calcifying and non-calcifying strains of E. huxleyi and cultures grown at various Ca2+ concentrations to alter calcite production. We show that calcification correlated to the consistent upregulation of a putative HCO3- transporter belonging to the solute carrier 4 (SLC4) family, a Ca2+/H+ exchanger belonging to the CAX family of exchangers and a vacuolar H+-ATPase. We also show that the coccolith-associated protein, GPA is downregulated in calcifying cells. The data provide strong evidence that these genes play key roles in E. huxleyi biomineralization. Based on the gene expression data and the current literature a working model for biomineralization-related ion transport in coccolithophores is presented. |
format |
Dataset |
author |
Mackinder, Luke C M Wheeler, Glen Schroeder, Declan C von Dassow, Peter Riebesell, Ulf Brownlee, Colin |
author_facet |
Mackinder, Luke C M Wheeler, Glen Schroeder, Declan C von Dassow, Peter Riebesell, Ulf Brownlee, Colin |
author_sort |
Mackinder, Luke C M |
title |
Expression of biomineralization-related ion transport genes in Emiliania huxleyi |
title_short |
Expression of biomineralization-related ion transport genes in Emiliania huxleyi |
title_full |
Expression of biomineralization-related ion transport genes in Emiliania huxleyi |
title_fullStr |
Expression of biomineralization-related ion transport genes in Emiliania huxleyi |
title_full_unstemmed |
Expression of biomineralization-related ion transport genes in Emiliania huxleyi |
title_sort |
expression of biomineralization-related ion transport genes in emiliania huxleyi |
publisher |
PANGAEA |
publishDate |
2011 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.832450 https://doi.org/10.1594/PANGAEA.832450 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Supplement to: Mackinder, Luke C M; Wheeler, Glen; Schroeder, Declan C; von Dassow, Peter; Riebesell, Ulf; Brownlee, Colin (2011): Expression of biomineralization-related ion transport genes in Emiliania huxleyi. Environmental Microbiology, 13(12), 3250-3265, https://doi.org/10.1111/j.1462-2920.2011.02561.x |
op_relation |
https://doi.pangaea.de/10.1594/PANGAEA.832450 https://doi.org/10.1594/PANGAEA.832450 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.832450 https://doi.org/10.1111/j.1462-2920.2011.02561.x |
_version_ |
1766158405474451456 |