Analysis of Pacific oyster larval proteome and its response to high-CO2
Most calcifying organisms show depressed metabolic, growth and calcification rates as symptoms to high-CO(2) due to ocean acidification (OA) process. Analysis of the global expression pattern of proteins (proteome analysis) represents a powerful tool to examine these physiological symptoms at molecu...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.823757 2024-09-15T18:03:16+00:00 Analysis of Pacific oyster larval proteome and its response to high-CO2 Dineshram, R Wong, Kevin K W Shu, Xiao Yu, Ziniu Qian, Pei Yuan Thiyagarajan, Vengatesen 2012 text/tab-separated-values, 1861 data points https://doi.pangaea.de/10.1594/PANGAEA.823757 https://doi.org/10.1594/PANGAEA.823757 en eng PANGAEA Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4 [webpage]. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.823757 https://doi.org/10.1594/PANGAEA.823757 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Dineshram, R; Wong, Kevin K W; Shu, Xiao; Yu, Ziniu; Qian, Pei Yuan; Thiyagarajan, Vengatesen (2012): Analysis of Pacific oyster larval proteome and its response to high-CO2. Marine Pollution Bulletin, 64(10), 2160-2167, https://doi.org/10.1016/j.marpolbul.2012.07.043 Alkalinity total Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Crassostrea gigas Duration number of days Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Growth/Morphology Identification Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Protein name Replicate Salinity Shell length Single species Species Spot intensity relative Temperature water Treatment Tropical Zooplankton dataset 2012 ftpangaea https://doi.org/10.1594/PANGAEA.82375710.1016/j.marpolbul.2012.07.043 2024-07-24T02:31:32Z Most calcifying organisms show depressed metabolic, growth and calcification rates as symptoms to high-CO(2) due to ocean acidification (OA) process. Analysis of the global expression pattern of proteins (proteome analysis) represents a powerful tool to examine these physiological symptoms at molecular level, but its applications are inadequate. To address this knowledge gap, 2-DE coupled with mass spectrophotometer was used to compare the global protein expression pattern of oyster larvae exposed to ambient and to high-CO(2). Exposure to OA resulted in marked reduction of global protein expression with a decrease or loss of 71 proteins (18% of the expressed proteins in control), indicating a wide-spread depression of metabolic genes expression in larvae reared under OA. This is, to our knowledge, the first proteome analysis that provides insights into the link between physiological suppression and protein down-regulation under OA in oyster larvae. Dataset Crassostrea gigas Ocean acidification Pacific oyster PANGAEA - Data Publisher for Earth & Environmental Science |
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Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Crassostrea gigas Duration number of days Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Growth/Morphology Identification Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Protein name Replicate Salinity Shell length Single species Species Spot intensity relative Temperature water Treatment Tropical Zooplankton |
spellingShingle |
Alkalinity total Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Crassostrea gigas Duration number of days Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Growth/Morphology Identification Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Protein name Replicate Salinity Shell length Single species Species Spot intensity relative Temperature water Treatment Tropical Zooplankton Dineshram, R Wong, Kevin K W Shu, Xiao Yu, Ziniu Qian, Pei Yuan Thiyagarajan, Vengatesen Analysis of Pacific oyster larval proteome and its response to high-CO2 |
topic_facet |
Alkalinity total Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Crassostrea gigas Duration number of days Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Growth/Morphology Identification Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Protein name Replicate Salinity Shell length Single species Species Spot intensity relative Temperature water Treatment Tropical Zooplankton |
description |
Most calcifying organisms show depressed metabolic, growth and calcification rates as symptoms to high-CO(2) due to ocean acidification (OA) process. Analysis of the global expression pattern of proteins (proteome analysis) represents a powerful tool to examine these physiological symptoms at molecular level, but its applications are inadequate. To address this knowledge gap, 2-DE coupled with mass spectrophotometer was used to compare the global protein expression pattern of oyster larvae exposed to ambient and to high-CO(2). Exposure to OA resulted in marked reduction of global protein expression with a decrease or loss of 71 proteins (18% of the expressed proteins in control), indicating a wide-spread depression of metabolic genes expression in larvae reared under OA. This is, to our knowledge, the first proteome analysis that provides insights into the link between physiological suppression and protein down-regulation under OA in oyster larvae. |
format |
Dataset |
author |
Dineshram, R Wong, Kevin K W Shu, Xiao Yu, Ziniu Qian, Pei Yuan Thiyagarajan, Vengatesen |
author_facet |
Dineshram, R Wong, Kevin K W Shu, Xiao Yu, Ziniu Qian, Pei Yuan Thiyagarajan, Vengatesen |
author_sort |
Dineshram, R |
title |
Analysis of Pacific oyster larval proteome and its response to high-CO2 |
title_short |
Analysis of Pacific oyster larval proteome and its response to high-CO2 |
title_full |
Analysis of Pacific oyster larval proteome and its response to high-CO2 |
title_fullStr |
Analysis of Pacific oyster larval proteome and its response to high-CO2 |
title_full_unstemmed |
Analysis of Pacific oyster larval proteome and its response to high-CO2 |
title_sort |
analysis of pacific oyster larval proteome and its response to high-co2 |
publisher |
PANGAEA |
publishDate |
2012 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.823757 https://doi.org/10.1594/PANGAEA.823757 |
genre |
Crassostrea gigas Ocean acidification Pacific oyster |
genre_facet |
Crassostrea gigas Ocean acidification Pacific oyster |
op_source |
Supplement to: Dineshram, R; Wong, Kevin K W; Shu, Xiao; Yu, Ziniu; Qian, Pei Yuan; Thiyagarajan, Vengatesen (2012): Analysis of Pacific oyster larval proteome and its response to high-CO2. Marine Pollution Bulletin, 64(10), 2160-2167, https://doi.org/10.1016/j.marpolbul.2012.07.043 |
op_relation |
Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4 [webpage]. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.823757 https://doi.org/10.1594/PANGAEA.823757 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.82375710.1016/j.marpolbul.2012.07.043 |
_version_ |
1810440785699536896 |