Rates of egg production, grazing and mortatlity of Calanus finmarchicus measured experimentally in the North Atlantic during the Maria S. Merian cruise MSM26, spring 2013

• Main Authors: Harmer, Rachel A, Lindeque, Pennie K
• Format: Dataset
• Language: English
• Published: PANGAEA 2013
• Subjects: Basin Scale Analysis; Synthesis and Integration; Calanus finmarchicus; egg production rate; standard deviation; egg production rate per female; fecal pellet production rate; fecal pellet production rate per female; female; EURO-BASIN; Maria S. Merian; MSM26; MSM26_127-9; North Atlantic Ocean; Time in hours; Treatment: duration; Treatment: temperature; WP2; WP-2 towed closing plankton net; North Atlantic
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.819657; https://doi.org/10.1594/PANGAEA.819657

An incubation experiment at five different temperatures was used to assess the potential for adaptation of Calanus finmarchicus to future warming of the ocean. During a short term (3 h) and long term (6 day) exposure of individual females to a gradient of temperature stress, egg production and fecal pellet production were monitored to indicate secondary production and grazing rates. A longer term (10 day) exposure to elevated temperatures followed by a return to ambient sea temperatures was used to assess the potential recovery of individuals exposed to temperature stress. Females were picked out from WP2 net samples and acclimatised in 2 L bottles of GFF filtered seawater with Thalassiosira weissflogii as prey for >48 h at ambient SST. Experimental bottles were filled with filtered seawater (GFF filtered from non-toxic seawater supply) and acclimated to experimental temperature overnight (0, 5, 10, 15 and 20 °C). Individual females were transferred into bottles using forceps and the bottles were inoculated with T. weissflogii to a final concentration of 5 µg chl L-1. Bottles were then placed into water baths and incubated for 3h or 6 d, and monitored for egg and fecal pellet production rates. A 10 day exposure experiment was used to test the potential for recovery from temperature stress, by returning females incubated at 5, 10, 15 and 20 °C back to 10 °C for 24 h and counting egg and fecal pellet production.