(Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity
The present study assessed and compared the oxidative and reductive biotransformation of brominated flame retardants, including established polybrominated diphenyl ethers (PBDEs) and emerging decabromodiphenyl ethane (DBDPE) using an in vitro system based on liver microsomes from various arctic mari...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.816176 2024-09-15T17:51:10+00:00 (Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity McKinney, Melissa A Dietz, Rune Sonne, Christian De Guise, Sylvain Skirnisson, Karl Karlsson, Karl Steingrimsson, Egill Letcher, Robert J MEDIAN LATITUDE: 63.555667 * MEDIAN LONGITUDE: -59.660333 * SOUTH-BOUND LATITUDE: 60.980000 * WEST-BOUND LONGITUDE: -93.710000 * NORTH-BOUND LATITUDE: 65.187000 * EAST-BOUND LONGITUDE: -19.500000 * DATE/TIME START: 2001-07-01T00:00:00 * DATE/TIME END: 2008-06-01T00:00:00 2011 text/tab-separated-values, 55 data points https://doi.pangaea.de/10.1594/PANGAEA.816176 https://doi.org/10.1594/PANGAEA.816176 en eng PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.816176 https://doi.org/10.1594/PANGAEA.816176 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: McKinney, Melissa A; Dietz, Rune; Sonne, Christian; De Guise, Sylvain; Skirnisson, Karl; Karlsson, Karl; Steingrimsson, Egill; Letcher, Robert J (2011): Comparative hepatic microsomal biotransformation of selected PBDEs, including decabromodiphenyl ether, and decabromodiphenyl ethane flame retardants in Arctic marine-feeding mammals. Environmental Toxicology and Chemistry, 30(7), 1506-1514, https://doi.org/10.1002/etc.535 7-ethoxyresorufin-O-deethylase activity per protein mass Age comment Area/locality Biological sample BIOS Cumberland_Sound DATE/TIME Event label Fluorescence microplate assay Hudson Bay Iceland International Polar Year (2007-2008) IPY Labrador Sea MULT Multiple investigations Proteins total standard deviation Sample ID Sample type Sex Species common name W_Hudson_Bay dataset 2011 ftpangaea https://doi.org/10.1594/PANGAEA.81617610.1002/etc.535 2024-07-24T02:31:32Z The present study assessed and compared the oxidative and reductive biotransformation of brominated flame retardants, including established polybrominated diphenyl ethers (PBDEs) and emerging decabromodiphenyl ethane (DBDPE) using an in vitro system based on liver microsomes from various arctic marine-feeding mammals: polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas), and ringed seal (Pusa hispida), and in laboratory rat as a mammalian model species. Greater depletion of fully brominated BDE209 (14-25% of 30pmol) and DBDPE (44-74% of 90pmol) occurred in individuals from all species relative to depletion of lower brominated PBDEs (BDEs 99,100, and 154; 0-3% of 30pmol). No evidence of simply debrominated metabolites was observed. Investigation of phenolic metabolites in rat and polar bear revealed formation of two phenolic, likely multiply debrominated, DBDPE metabolites in polar bear and one phenolic BDE154 metabolite in polar bear and rat microsomes. For BDE209 and DBDPE, observed metabolite concentrations were low to nondetectable, despite substantial parent depletion. These findings suggested possible underestimation of the ecosystem burden of total-BDE209, as well as its transformation products, and a need for research to identify and characterize the persistence and toxicity of major BDE209 metabolites. Similar cause for concern may exist regarding DBDPE, given similarities of physicochemical and environmental behavior to BDE209, current evidence of biotransformation, and increasing use of DBDPE as a replacement for BDE209. Dataset Arctic Beluga Beluga whale Beluga* Delphinapterus leucas Hudson Bay Iceland International Polar Year IPY Labrador Sea Pusa hispida ringed seal Ursus maritimus PANGAEA - Data Publisher for Earth & Environmental Science ENVELOPE(-93.710000,-19.500000,65.187000,60.980000) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
7-ethoxyresorufin-O-deethylase activity per protein mass Age comment Area/locality Biological sample BIOS Cumberland_Sound DATE/TIME Event label Fluorescence microplate assay Hudson Bay Iceland International Polar Year (2007-2008) IPY Labrador Sea MULT Multiple investigations Proteins total standard deviation Sample ID Sample type Sex Species common name W_Hudson_Bay |
spellingShingle |
7-ethoxyresorufin-O-deethylase activity per protein mass Age comment Area/locality Biological sample BIOS Cumberland_Sound DATE/TIME Event label Fluorescence microplate assay Hudson Bay Iceland International Polar Year (2007-2008) IPY Labrador Sea MULT Multiple investigations Proteins total standard deviation Sample ID Sample type Sex Species common name W_Hudson_Bay McKinney, Melissa A Dietz, Rune Sonne, Christian De Guise, Sylvain Skirnisson, Karl Karlsson, Karl Steingrimsson, Egill Letcher, Robert J (Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity |
topic_facet |
7-ethoxyresorufin-O-deethylase activity per protein mass Age comment Area/locality Biological sample BIOS Cumberland_Sound DATE/TIME Event label Fluorescence microplate assay Hudson Bay Iceland International Polar Year (2007-2008) IPY Labrador Sea MULT Multiple investigations Proteins total standard deviation Sample ID Sample type Sex Species common name W_Hudson_Bay |
description |
The present study assessed and compared the oxidative and reductive biotransformation of brominated flame retardants, including established polybrominated diphenyl ethers (PBDEs) and emerging decabromodiphenyl ethane (DBDPE) using an in vitro system based on liver microsomes from various arctic marine-feeding mammals: polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas), and ringed seal (Pusa hispida), and in laboratory rat as a mammalian model species. Greater depletion of fully brominated BDE209 (14-25% of 30pmol) and DBDPE (44-74% of 90pmol) occurred in individuals from all species relative to depletion of lower brominated PBDEs (BDEs 99,100, and 154; 0-3% of 30pmol). No evidence of simply debrominated metabolites was observed. Investigation of phenolic metabolites in rat and polar bear revealed formation of two phenolic, likely multiply debrominated, DBDPE metabolites in polar bear and one phenolic BDE154 metabolite in polar bear and rat microsomes. For BDE209 and DBDPE, observed metabolite concentrations were low to nondetectable, despite substantial parent depletion. These findings suggested possible underestimation of the ecosystem burden of total-BDE209, as well as its transformation products, and a need for research to identify and characterize the persistence and toxicity of major BDE209 metabolites. Similar cause for concern may exist regarding DBDPE, given similarities of physicochemical and environmental behavior to BDE209, current evidence of biotransformation, and increasing use of DBDPE as a replacement for BDE209. |
format |
Dataset |
author |
McKinney, Melissa A Dietz, Rune Sonne, Christian De Guise, Sylvain Skirnisson, Karl Karlsson, Karl Steingrimsson, Egill Letcher, Robert J |
author_facet |
McKinney, Melissa A Dietz, Rune Sonne, Christian De Guise, Sylvain Skirnisson, Karl Karlsson, Karl Steingrimsson, Egill Letcher, Robert J |
author_sort |
McKinney, Melissa A |
title |
(Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity |
title_short |
(Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity |
title_full |
(Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity |
title_fullStr |
(Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity |
title_full_unstemmed |
(Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity |
title_sort |
(tables 1,2) polar bear (ursus maritimus), beluga whale (delphinapterus leucas) and ringed seal (pusa hispida) liver microsomal protein content and erod activity |
publisher |
PANGAEA |
publishDate |
2011 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.816176 https://doi.org/10.1594/PANGAEA.816176 |
op_coverage |
MEDIAN LATITUDE: 63.555667 * MEDIAN LONGITUDE: -59.660333 * SOUTH-BOUND LATITUDE: 60.980000 * WEST-BOUND LONGITUDE: -93.710000 * NORTH-BOUND LATITUDE: 65.187000 * EAST-BOUND LONGITUDE: -19.500000 * DATE/TIME START: 2001-07-01T00:00:00 * DATE/TIME END: 2008-06-01T00:00:00 |
long_lat |
ENVELOPE(-93.710000,-19.500000,65.187000,60.980000) |
genre |
Arctic Beluga Beluga whale Beluga* Delphinapterus leucas Hudson Bay Iceland International Polar Year IPY Labrador Sea Pusa hispida ringed seal Ursus maritimus |
genre_facet |
Arctic Beluga Beluga whale Beluga* Delphinapterus leucas Hudson Bay Iceland International Polar Year IPY Labrador Sea Pusa hispida ringed seal Ursus maritimus |
op_source |
Supplement to: McKinney, Melissa A; Dietz, Rune; Sonne, Christian; De Guise, Sylvain; Skirnisson, Karl; Karlsson, Karl; Steingrimsson, Egill; Letcher, Robert J (2011): Comparative hepatic microsomal biotransformation of selected PBDEs, including decabromodiphenyl ether, and decabromodiphenyl ethane flame retardants in Arctic marine-feeding mammals. Environmental Toxicology and Chemistry, 30(7), 1506-1514, https://doi.org/10.1002/etc.535 |
op_relation |
https://doi.pangaea.de/10.1594/PANGAEA.816176 https://doi.org/10.1594/PANGAEA.816176 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.81617610.1002/etc.535 |
_version_ |
1810293002950672384 |