qPCR counts of mRNA for hepatic reference gene selection in juvenile and adult female Atlantic salmon from perturbation experiments under artificial temperature conditions
The use of quantitative real-time polymerase chain reaction (qPCR) has become widespread due to its specificity, sensitivity and apparent ease of use. However, experimental error can be introduced at many stages during sample processing and analysis, and for this reason qPCR data are often normalise...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.810131 2023-05-15T15:31:18+02:00 qPCR counts of mRNA for hepatic reference gene selection in juvenile and adult female Atlantic salmon from perturbation experiments under artificial temperature conditions Anderson, Kelli Elizur, Abigail DATE/TIME START: 2007-01-08T00:00:00 * DATE/TIME END: 2009-01-02T00:00:00 2013-04-11 application/zip, 2 datasets https://doi.pangaea.de/10.1594/PANGAEA.810131 https://doi.org/10.1594/PANGAEA.810131 en eng PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.810131 https://doi.org/10.1594/PANGAEA.810131 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Anderson, Kelli; Elizur, Abigail (2012): Hepatic reference gene selection in adult and juvenile female Atlantic salmon at normal and elevated temperatures. BMC Research Notes, 5(21), 1-9, https://doi.org/10.1186/1756-0500-5-21 Dataset 2013 ftpangaea https://doi.org/10.1594/PANGAEA.810131 https://doi.org/10.1186/1756-0500-5-21 2023-01-20T07:32:57Z The use of quantitative real-time polymerase chain reaction (qPCR) has become widespread due to its specificity, sensitivity and apparent ease of use. However, experimental error can be introduced at many stages during sample processing and analysis, and for this reason qPCR data are often normalised to an internal reference gene. The present study used three freely available algorithms (GeNorm, NormFinder and BestKeeper) to assess the stability of hepatically expressed candidate reference genes (Hprt1, Tbp, Ef1a and b-tubulin) in two experiments. In the first, female Atlantic salmon (Salmo salar) broodstock of different ages were reared at either 14 or 22°C for an entire reproductive season, therefore a reference gene that does not respond to thermal challenge or reproductive condition was sought. In the second, estrogen treated juvenile salmon were maintained at the same temperatures for 14 days and a reference gene that does not respond to temperature or estrogen was required. Additionally, we performed independent statistic analysis to validate the outputs obtained from the program based analysis. Dataset Atlantic salmon Salmo salar PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
description |
The use of quantitative real-time polymerase chain reaction (qPCR) has become widespread due to its specificity, sensitivity and apparent ease of use. However, experimental error can be introduced at many stages during sample processing and analysis, and for this reason qPCR data are often normalised to an internal reference gene. The present study used three freely available algorithms (GeNorm, NormFinder and BestKeeper) to assess the stability of hepatically expressed candidate reference genes (Hprt1, Tbp, Ef1a and b-tubulin) in two experiments. In the first, female Atlantic salmon (Salmo salar) broodstock of different ages were reared at either 14 or 22°C for an entire reproductive season, therefore a reference gene that does not respond to thermal challenge or reproductive condition was sought. In the second, estrogen treated juvenile salmon were maintained at the same temperatures for 14 days and a reference gene that does not respond to temperature or estrogen was required. Additionally, we performed independent statistic analysis to validate the outputs obtained from the program based analysis. |
format |
Dataset |
author |
Anderson, Kelli Elizur, Abigail |
spellingShingle |
Anderson, Kelli Elizur, Abigail qPCR counts of mRNA for hepatic reference gene selection in juvenile and adult female Atlantic salmon from perturbation experiments under artificial temperature conditions |
author_facet |
Anderson, Kelli Elizur, Abigail |
author_sort |
Anderson, Kelli |
title |
qPCR counts of mRNA for hepatic reference gene selection in juvenile and adult female Atlantic salmon from perturbation experiments under artificial temperature conditions |
title_short |
qPCR counts of mRNA for hepatic reference gene selection in juvenile and adult female Atlantic salmon from perturbation experiments under artificial temperature conditions |
title_full |
qPCR counts of mRNA for hepatic reference gene selection in juvenile and adult female Atlantic salmon from perturbation experiments under artificial temperature conditions |
title_fullStr |
qPCR counts of mRNA for hepatic reference gene selection in juvenile and adult female Atlantic salmon from perturbation experiments under artificial temperature conditions |
title_full_unstemmed |
qPCR counts of mRNA for hepatic reference gene selection in juvenile and adult female Atlantic salmon from perturbation experiments under artificial temperature conditions |
title_sort |
qpcr counts of mrna for hepatic reference gene selection in juvenile and adult female atlantic salmon from perturbation experiments under artificial temperature conditions |
publisher |
PANGAEA |
publishDate |
2013 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.810131 https://doi.org/10.1594/PANGAEA.810131 |
op_coverage |
DATE/TIME START: 2007-01-08T00:00:00 * DATE/TIME END: 2009-01-02T00:00:00 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Supplement to: Anderson, Kelli; Elizur, Abigail (2012): Hepatic reference gene selection in adult and juvenile female Atlantic salmon at normal and elevated temperatures. BMC Research Notes, 5(21), 1-9, https://doi.org/10.1186/1756-0500-5-21 |
op_relation |
https://doi.pangaea.de/10.1594/PANGAEA.810131 https://doi.org/10.1594/PANGAEA.810131 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.810131 https://doi.org/10.1186/1756-0500-5-21 |
_version_ |
1766361791448743936 |