Seawater carbonate chemistry and expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera during experiments, 2012
Ocean acidification is an ongoing threat for marine organisms due to the increasing atmospheric CO2 concentration. Seawater acidification has a serious impact on physiologic processes in marine organisms at all life stages. On the other hand, potential tolerance to external pH changes has been repor...
Main Authors: | , , , |
---|---|
Format: | Dataset |
Language: | English |
Published: |
PANGAEA
2012
|
Subjects: | |
Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.779704 https://doi.org/10.1594/PANGAEA.779704 |
id |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.779704 |
---|---|
record_format |
openpolar |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.779704 2024-09-15T18:28:00+00:00 Seawater carbonate chemistry and expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera during experiments, 2012 Nakamura, Masoko Morita, Masaya Kurihara, Haruko Mitarai, Satoshi 2012 text/tab-separated-values, 256 data points https://doi.pangaea.de/10.1594/PANGAEA.779704 https://doi.org/10.1594/PANGAEA.779704 en eng PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.779704 https://doi.org/10.1594/PANGAEA.779704 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Nakamura, Masoko; Morita, Masaya; Kurihara, Haruko; Mitarai, Satoshi (2012): Expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera under prospective acidified conditions over the next several decades. Biology Open, 1(2), 75-81, https://doi.org/10.1242/bio.2011036 Acropora digitifera heat shock factor 1 expression standard error heat shock protein 70 expression heat shock protein 90 expression Alkalinity total Animalia Aragonite saturation state standard deviation Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cnidaria Coast and continental shelf EPOCA EUR-OCEANS European network of excellence for Ocean Ecosystems Analysis European Project on Ocean Acidification Experimental treatment dataset 2012 ftpangaea https://doi.org/10.1594/PANGAEA.77970410.1242/bio.2011036 2024-07-24T02:31:31Z Ocean acidification is an ongoing threat for marine organisms due to the increasing atmospheric CO2 concentration. Seawater acidification has a serious impact on physiologic processes in marine organisms at all life stages. On the other hand, potential tolerance to external pH changes has been reported in coral larvae. Information about the possible mechanisms underlying such tolerance responses, however, is scarce. In the present study, we examined the effects of acidified seawater on the larvae of Acropora digitifera at the molecular level. We targeted two heat shock proteins, Hsp70 and Hsp90, and a heat shock transcription factor, Hsf1, because of their importance in stress responses and in early life developmental stages. Coral larvae were maintained under the ambient and elevated CO2 conditions that are expected to occur within next 100 years, and then we evaluated the expression of hsps and hsf1 by quantitative real-time polymerase chain reaction (PCR). Expression levels of these molecules significantly differed among target genes, but they did not change significantly between CO2conditions. These findings indicate that the expression of hsps is not changed due to external pH changes, and suggest that tolerance to acidified seawater in coral larvae may not be related to hsp expression. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Acropora digitifera heat shock factor 1 expression standard error heat shock protein 70 expression heat shock protein 90 expression Alkalinity total Animalia Aragonite saturation state standard deviation Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cnidaria Coast and continental shelf EPOCA EUR-OCEANS European network of excellence for Ocean Ecosystems Analysis European Project on Ocean Acidification Experimental treatment |
spellingShingle |
Acropora digitifera heat shock factor 1 expression standard error heat shock protein 70 expression heat shock protein 90 expression Alkalinity total Animalia Aragonite saturation state standard deviation Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cnidaria Coast and continental shelf EPOCA EUR-OCEANS European network of excellence for Ocean Ecosystems Analysis European Project on Ocean Acidification Experimental treatment Nakamura, Masoko Morita, Masaya Kurihara, Haruko Mitarai, Satoshi Seawater carbonate chemistry and expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera during experiments, 2012 |
topic_facet |
Acropora digitifera heat shock factor 1 expression standard error heat shock protein 70 expression heat shock protein 90 expression Alkalinity total Animalia Aragonite saturation state standard deviation Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cnidaria Coast and continental shelf EPOCA EUR-OCEANS European network of excellence for Ocean Ecosystems Analysis European Project on Ocean Acidification Experimental treatment |
description |
Ocean acidification is an ongoing threat for marine organisms due to the increasing atmospheric CO2 concentration. Seawater acidification has a serious impact on physiologic processes in marine organisms at all life stages. On the other hand, potential tolerance to external pH changes has been reported in coral larvae. Information about the possible mechanisms underlying such tolerance responses, however, is scarce. In the present study, we examined the effects of acidified seawater on the larvae of Acropora digitifera at the molecular level. We targeted two heat shock proteins, Hsp70 and Hsp90, and a heat shock transcription factor, Hsf1, because of their importance in stress responses and in early life developmental stages. Coral larvae were maintained under the ambient and elevated CO2 conditions that are expected to occur within next 100 years, and then we evaluated the expression of hsps and hsf1 by quantitative real-time polymerase chain reaction (PCR). Expression levels of these molecules significantly differed among target genes, but they did not change significantly between CO2conditions. These findings indicate that the expression of hsps is not changed due to external pH changes, and suggest that tolerance to acidified seawater in coral larvae may not be related to hsp expression. |
format |
Dataset |
author |
Nakamura, Masoko Morita, Masaya Kurihara, Haruko Mitarai, Satoshi |
author_facet |
Nakamura, Masoko Morita, Masaya Kurihara, Haruko Mitarai, Satoshi |
author_sort |
Nakamura, Masoko |
title |
Seawater carbonate chemistry and expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera during experiments, 2012 |
title_short |
Seawater carbonate chemistry and expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera during experiments, 2012 |
title_full |
Seawater carbonate chemistry and expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera during experiments, 2012 |
title_fullStr |
Seawater carbonate chemistry and expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera during experiments, 2012 |
title_full_unstemmed |
Seawater carbonate chemistry and expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera during experiments, 2012 |
title_sort |
seawater carbonate chemistry and expression of hsp70, hsp90 and hsf1 in the reef coral acropora digitifera during experiments, 2012 |
publisher |
PANGAEA |
publishDate |
2012 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.779704 https://doi.org/10.1594/PANGAEA.779704 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Supplement to: Nakamura, Masoko; Morita, Masaya; Kurihara, Haruko; Mitarai, Satoshi (2012): Expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera under prospective acidified conditions over the next several decades. Biology Open, 1(2), 75-81, https://doi.org/10.1242/bio.2011036 |
op_relation |
https://doi.pangaea.de/10.1594/PANGAEA.779704 https://doi.org/10.1594/PANGAEA.779704 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.77970410.1242/bio.2011036 |
_version_ |
1810469308837396480 |