Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (Table 1)
The taxonomy of Antarctic fishes has been predominantly based on morphological characteristics rather than on genetic criteria. A typical example is the Notothenia group, which includes N. coriiceps Richardson, 1844, N. neglecta Nybelin, 1951 and N. rossii Richardson, 1844. The Polymerase Chain Reac...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.778124 2023-05-15T13:55:15+02:00 Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (Table 1) Machado, Cintia Shimada, Marcia Kiyoe Fragoso, Stênio Perdigao Fanta, Edith Kawall, Helena G Rodrigues, Edson Donatti, Lucélia 2012-03-23 text/tab-separated-values, 20 data points https://doi.pangaea.de/10.1594/PANGAEA.778124 https://doi.org/10.1594/PANGAEA.778124 en eng PANGAEA Barrera-Oro, Esteban; Eastman, Joseph T; Moreira, Eugenia (2012): Phenotypic plasticity in the Antarctic nototheniid fish Trematomus newnesi: a guide to the identification of typical, large mouth and intermediate morphs. Polar Biology, 35(7), 1047-1056, https://doi.org/10.1007/s00300-011-1152-5 Oleinik, Alla G; Skurikhina, Lubov A; Brykov, Vladimir A (2007): Divergence of Salvelinus species from northeastern Asia based on mitochondrial DNA. Ecology of Freshwater Fish, 16(1), 87-98, https://doi.org/10.1111/j.1600-0633.2006.00187.x Oleinik, Alla G; Skurikhina, Lubov A; Frolov, Sergei V; Brykov, Vladimir A; Chereshnev, Igor A (2004): Differences Between two Subspecies of Dolly Varden, Salvelinus malma, Revealed by RFLP—PCR Analysis of Mitochondrial DNA. Environmental Biology of Fishes, 69(1-4), 449-459, https://doi.org/10.1023/B:EBFI.0000022897.26755.da https://doi.pangaea.de/10.1594/PANGAEA.778124 https://doi.org/10.1594/PANGAEA.778124 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Machado, Cintia; Shimada, Marcia Kiyoe; Fragoso, Stênio Perdigao; Fanta, Edith; Kawall, Helena G; Rodrigues, Edson; Donatti, Lucélia (in prep.): Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern. data submission http://issues.pangaea.de/browse/PDI-1590, hdl:10013/epic.38971.d002 Characteristic Notothenia coriiceps Notothenia neglecta Notothenia rossii Dataset 2012 ftpangaea https://doi.org/10.1594/PANGAEA.778124 https://doi.org/10.1007/s00300-011-1152-5 https://doi.org/10.1111/j.1600-0633.2006.00187.x https://doi.org/10.1023/B:EBFI.0000022897.26755.da 2023-01-20T08:53:39Z The taxonomy of Antarctic fishes has been predominantly based on morphological characteristics rather than on genetic criteria. A typical example is the Notothenia group, which includes N. coriiceps Richardson, 1844, N. neglecta Nybelin, 1951 and N. rossii Richardson, 1844. The Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP) technique was used to determine whether N. coriiceps Richardson, 1844 and N. neglecta Nybelin, 1951 are different or whether they are the same species with morphological, physiological and behavioural variability. N. rossii was used as control. Mitochondrial DNA (mtDNA) was isolated from muscle specimens of N. coriiceps Richardson, 1844, N. neglecta Nybelin, 1951 and N. rossii, which were collected in Admiralty Bay, King George Island. The DNA was used to amplify a fragment (690 base pairs) of the mitochondrial gene coding region of NADH dehydrogenase subunit 2. Further, the amplicon was digested with the following restriction enzymes: DdeI, HindIII and RsaI. The results showed a variation of the digestion pattern of the fragment amplified between N. rossii, and N. coriiceps Richardson, 1844 or N. neglecta Nybelin, 1951. However, no differences were found between N. coriiceps Richardson, 1844 and N. neglecta Nybelin, 1951, on the grounds of the same genetic pattern shown by the two fish. Dataset Antarc* Antarctic King George Island Notothenia rossii Polar Biology PANGAEA - Data Publisher for Earth & Environmental Science Admiralty Bay Antarctic King George Island |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Characteristic Notothenia coriiceps Notothenia neglecta Notothenia rossii |
spellingShingle |
Characteristic Notothenia coriiceps Notothenia neglecta Notothenia rossii Machado, Cintia Shimada, Marcia Kiyoe Fragoso, Stênio Perdigao Fanta, Edith Kawall, Helena G Rodrigues, Edson Donatti, Lucélia Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (Table 1) |
topic_facet |
Characteristic Notothenia coriiceps Notothenia neglecta Notothenia rossii |
description |
The taxonomy of Antarctic fishes has been predominantly based on morphological characteristics rather than on genetic criteria. A typical example is the Notothenia group, which includes N. coriiceps Richardson, 1844, N. neglecta Nybelin, 1951 and N. rossii Richardson, 1844. The Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP) technique was used to determine whether N. coriiceps Richardson, 1844 and N. neglecta Nybelin, 1951 are different or whether they are the same species with morphological, physiological and behavioural variability. N. rossii was used as control. Mitochondrial DNA (mtDNA) was isolated from muscle specimens of N. coriiceps Richardson, 1844, N. neglecta Nybelin, 1951 and N. rossii, which were collected in Admiralty Bay, King George Island. The DNA was used to amplify a fragment (690 base pairs) of the mitochondrial gene coding region of NADH dehydrogenase subunit 2. Further, the amplicon was digested with the following restriction enzymes: DdeI, HindIII and RsaI. The results showed a variation of the digestion pattern of the fragment amplified between N. rossii, and N. coriiceps Richardson, 1844 or N. neglecta Nybelin, 1951. However, no differences were found between N. coriiceps Richardson, 1844 and N. neglecta Nybelin, 1951, on the grounds of the same genetic pattern shown by the two fish. |
format |
Dataset |
author |
Machado, Cintia Shimada, Marcia Kiyoe Fragoso, Stênio Perdigao Fanta, Edith Kawall, Helena G Rodrigues, Edson Donatti, Lucélia |
author_facet |
Machado, Cintia Shimada, Marcia Kiyoe Fragoso, Stênio Perdigao Fanta, Edith Kawall, Helena G Rodrigues, Edson Donatti, Lucélia |
author_sort |
Machado, Cintia |
title |
Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (Table 1) |
title_short |
Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (Table 1) |
title_full |
Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (Table 1) |
title_fullStr |
Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (Table 1) |
title_full_unstemmed |
Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (Table 1) |
title_sort |
molecular differentiation by pcr-rflp technique shows that antarctic fishes of the genus notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (table 1) |
publisher |
PANGAEA |
publishDate |
2012 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.778124 https://doi.org/10.1594/PANGAEA.778124 |
geographic |
Admiralty Bay Antarctic King George Island |
geographic_facet |
Admiralty Bay Antarctic King George Island |
genre |
Antarc* Antarctic King George Island Notothenia rossii Polar Biology |
genre_facet |
Antarc* Antarctic King George Island Notothenia rossii Polar Biology |
op_source |
Supplement to: Machado, Cintia; Shimada, Marcia Kiyoe; Fragoso, Stênio Perdigao; Fanta, Edith; Kawall, Helena G; Rodrigues, Edson; Donatti, Lucélia (in prep.): Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern. data submission http://issues.pangaea.de/browse/PDI-1590, hdl:10013/epic.38971.d002 |
op_relation |
Barrera-Oro, Esteban; Eastman, Joseph T; Moreira, Eugenia (2012): Phenotypic plasticity in the Antarctic nototheniid fish Trematomus newnesi: a guide to the identification of typical, large mouth and intermediate morphs. Polar Biology, 35(7), 1047-1056, https://doi.org/10.1007/s00300-011-1152-5 Oleinik, Alla G; Skurikhina, Lubov A; Brykov, Vladimir A (2007): Divergence of Salvelinus species from northeastern Asia based on mitochondrial DNA. Ecology of Freshwater Fish, 16(1), 87-98, https://doi.org/10.1111/j.1600-0633.2006.00187.x Oleinik, Alla G; Skurikhina, Lubov A; Frolov, Sergei V; Brykov, Vladimir A; Chereshnev, Igor A (2004): Differences Between two Subspecies of Dolly Varden, Salvelinus malma, Revealed by RFLP—PCR Analysis of Mitochondrial DNA. Environmental Biology of Fishes, 69(1-4), 449-459, https://doi.org/10.1023/B:EBFI.0000022897.26755.da https://doi.pangaea.de/10.1594/PANGAEA.778124 https://doi.org/10.1594/PANGAEA.778124 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.778124 https://doi.org/10.1007/s00300-011-1152-5 https://doi.org/10.1111/j.1600-0633.2006.00187.x https://doi.org/10.1023/B:EBFI.0000022897.26755.da |
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