qPCR counts of mRNA for hepatic reference gene selection in adult female Atlantic salmon from perturbation experiments under artificial temperature conditions

• Main Authors: Anderson, Kelli, Elizur, Abigail
• Format: Dataset
• Language: English
• Published: PANGAEA 2011
• Subjects: DATE/TIME; Event label; Exp_Tank_4m3; Experimental tank (4 cubic metre) under artificial conditions; Fluorescence-based quantitative real-time PCR (qPCR); Life Cycle: state; qPCR counts; mean; ratio; standard error of mean; SALTAS_Experimental_Tank(14degC)_April_2008; SALTAS_Experimental_Tank(14degC)_August_2007; SALTAS_Experimental_Tank(14degC)_February_2008; SALTAS_Experimental_Tank(14degC)_January_2008; SALTAS_Experimental_Tank(14degC)_March_2008; SALTAS_Experimental_Tank(14degC)_November_2007; SALTAS_Experimental_Tank(22degC)_April_2008; SALTAS_Experimental_Tank(22degC)_February_2008; SALTAS_Experimental_Tank(22degC)_March_2008; Treatment: temperature; Atlantic salmon
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.772212; https://doi.org/10.1594/PANGAEA.772212

The dataset contains raw data (quantification cycle) for a study which determined the most suitable hepatic reference genes for normalisation of qPCR data orginating from adult (entire reproductive season) Atlantic salmon (14 days) exposed to 14 and 22 degrees C. These results will be useful for anyone wanting to study the effects of climate change/elevated temperature on reproductive physiology of fish (and perhaphs other vertebrates). In addition, a target gene (vitellogenin) has normalised using an inappropriate and an 'ideal' reference gene to demonstrate the consequences of using an unstable reference gene for normalisation. For the adult experiment, maiden and repeat adult females were held at the Salmon Enterprises of Tasmania (SALTAS) Wayatinah Hatchery (Tasmania, Australia) at ambient temperature and photoperiod in either 200 (maidens) or 50 (repeats) m3 circular tanks at stocking densities of 12-18, and 24-36 kg m-3 for maidens and repeats, respectively, until transfered to the experimental tanks.